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Serratia marcescens nlm280 and its application as an insecticide

A technology of Serratia marcescens and bacterial suspension, applied in the direction of insecticide, application, biocide, etc., can solve the problems of easy drug resistance, poor stability, narrow insecticidal spectrum, etc., and achieve potential application value Effect

Inactive Publication Date: 2016-08-24
CHINA NAT RICE RES INST
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

These insecticides have limitations in varying degrees in production practice, such as narrow insecticidal spectrum, poor stability, and easy to produce drug resistance, etc.
So far, there are no reports on the isolation of Serratia marcescens from rice pests and the isolation and pathogenic mechanism of pathogenic bacteria resources that have pathogenic effects on rice pests Nilaparvata lugens and Chilo suppressalis

Method used

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  • Serratia marcescens nlm280 and its application as an insecticide
  • Serratia marcescens nlm280 and its application as an insecticide
  • Serratia marcescens nlm280 and its application as an insecticide

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0029] Screening, identification and preservation of embodiment 1, bacterial strain N1M280

[0030] (1) Screening of strains

[0031] The brown planthopper natural pathogenic body (body color is all red) of the brown planthopper population Mudgo raised in the Fuyang laboratory of China Rice Research Institute was surface-sterilized with 75% ethanol aqueous solution for 5 minutes, and the body was ground into powder. Bacterial water was serially diluted (the specific dilution gradient is 10 times, 100 times, and 1000 times), and the 1000-fold dilution was taken and spread on the LB solid medium, cultured at 28°C for 12 hours, and a single colony was obtained, and the red single The colony is designated as strain N1M280.

[0032] (2) Identification of strains

[0033] The physiological and biochemical characteristics of the strain are:

[0034] Spread the strain N1M280 on the LB solid medium, activate it at 28°C for 24 hours, see the plate growth of the strain N1M280 figure ...

Embodiment 2

[0040] Embodiment 2, the preparation of bacterial suspension

[0041] (1) Incline cultivation

[0042] Serratia marcescens N1M280 was inoculated onto LB solid medium and kept at 28°C for 24 hours to obtain slants.

[0043] (2) Seed culture: pick a single colony from the slant and inoculate it in 3 mL of LB liquid medium, keep the temperature at 28° C., shake at 200 rpm for 24 hours, and obtain the seed liquid.

[0044] (3) Fermentation culture: Inoculate 500 μL of seed liquid into 50 mL of LB liquid medium for expanded cultivation, keep the temperature at 28°C, shake and cultivate at 200 rpm for 8 hours, and when the OD600 value of the bacteria reaches 1.0, obtain the fermentation culture medium, and use LB Adjust the cell density of the culture medium to l×l0 8 CFU / mL, made into bacterial suspension.

Embodiment 3

[0045] Embodiment 3: the pathogenicity of the bacterial suspension of Serratia marcescens N1M280 to brown planthopper

[0046] (1), the pathogenicity of the strain detected by injection method:

[0047] Select 3-4 instar N. lugens nymphs, and use an eppendorf microinjector (model TransferMan NK2, manufacturer Shanghai Eppende Co., Ltd.), to inject 0.1 μL of the bacterial suspension prepared by the method in Example 2 into each N. lugens planthopper. Where the base is soft, see Figure 4 , with the injection of LB liquid medium as a control, the brown planthopper was inoculated on the rice variety (Taichung Native1) TN1 at the tillering stage after the injection, and each rice plant was covered with a cage, and each cage received 50 brown planthoppers after the injection. Repeat three times. Place the cage in a large basin with water, and control the indoor temperature at 25±1°C and humidity around 80%. The dead insects caused by the injection operation were eliminated withi...

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Abstract

The invention discloses a Serratia marcescens N1M280 and its application as an insecticide. The bacterial suspension obtained from the fermented culture of Serratia marcescens N1M280 or the supernatant after centrifugation of the bacterial suspension can be applied to brown planthoppers or The prevention and control of the rice borer; the results of injecting and feeding the bacterial suspension or fermentation supernatant to brown planthopper showed that the lethality rate of the bacterial suspension injection to brown planthopper could reach 100% in 2 days, and the bacterial suspension was fed for 3 days. The one-day lethality rate was 79.8%; the treatment of the supernatant of the fermentation broth of the strain below 55°C did not affect its pathogenic effect, and the lethality rate of the injection of the fermentation supernatant could reach 97.2% after 2 days, and the effect of feeding the fermentation supernatant lagged behind , the lethality rate reached 84.2% in 6 days. After spraying the rice with the bacterial suspension and the fermentation supernatant, feeding the rice stem borer, the lethality rate of the stem borer can reach more than 80.0% in 4 days.

Description

(1) Technical field [0001] The invention relates to a new bacterial strain-Serratia marcescens, which has pathogenic effect on rice pests (BPH and Chilo suppressalis), and the application of the bacteria in the prevention and control of Brown planthopper and Chilo suppressalis. (2) Background technology [0002] Rice is one of the main food crops in my country, and it is the staple food of more than half of the world's population. Therefore, it occupies an extremely important position in food safety production. However, rice is also one of the food crops most severely attacked by insect pests. Pests and diseases in rice fields across the country occur on an area of ​​more than 100 million hectares per year, and can cause about 600,000 tons of rice loss every year. Therefore, effective control of rice pests is of great significance to increase rice production. [0003] Brown planthopper (Nilaparvata lμgens ) (Brown planthopper) belongs to the family Planthopper of the or...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20A01N63/02A01N63/00A01P7/04C12R1/43
Inventor 王渭霞李凯龙陈洋赖凤香傅强
Owner CHINA NAT RICE RES INST