Method for carrying out virus removal on prunus salicina during tissue culture and rapid propagation

A technology of hollow plum and hollow plum, which is applied in the field of rapid propagation and detoxification of hollow plum tissue culture, can solve the problems of difficult virus removal, difficulty in obtaining success, and inability to completely inhibit virus inactivation, and achieve bright color, good appearance, and enhanced disease resistance. Effect

Inactive Publication Date: 2014-10-29
GUIZHOU YANHE WUJIANG BIOTECH DEV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The above-mentioned agents can inhibit the synthesis of viruses in plants or leaves in vitro to a certain extent, but they cannot completely inhibit the virus and make it inactivated
[0007] The above methods have disadvantages when used alone. Some viruses are difficult to remove, and it is difficult to succeed by using one of them alone. It is a simple, quick and efficient method to deal with poison

Method used

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  • Method for carrying out virus removal on prunus salicina during tissue culture and rapid propagation
  • Method for carrying out virus removal on prunus salicina during tissue culture and rapid propagation
  • Method for carrying out virus removal on prunus salicina during tissue culture and rapid propagation

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Embodiment 1

[0031] A method for rapid propagation and detoxification of hollow plum tissue culture, comprising the steps of:

[0032] 1) Selection and pretreatment of implants: Cut off the strong branches on the sandy hollow plum plants, treat them with rooting agents, place the cuttings on the treated artificial substrate, and transfer them to the cultivation room after they survive and grow new shoots. Cultivate for 16 hours at 38°C and 2500 Lux light conditions, then culture for 8 hours at room temperature and dark conditions, and co-cultivate for 30 days;

[0033] 2) stripping of the stem tip: get the sturdy stem buds of the hollow plum after the pretreatment in step 1), the length is 15mm, strip off the leaves, rinse and carry out routine disinfection, then place the stem buds under a 40-fold dissecting microscope, use Peel off the leaves and leaf primordium with a non-toxic dissecting needle, separate the meristem at the top of the stem bud, the length is 1.0mm, and then quickly ino...

Embodiment 2

[0041] A method for rapid propagation and detoxification of hollow plum tissue culture, comprising the steps of:

[0042] 1) Selection and pretreatment of implants: Cut off the strong branches on the sandy hollow plum plants, treat them with rooting agents, place the cuttings on the treated artificial substrate, and transfer them to the cultivation room after they survive and grow new shoots. Cultivate at 40°C for 14 hours under 3000 Lux light conditions, then at room temperature and dark conditions for 10 hours, and co-cultivate for 28 days;

[0043] 2) stripping of the stem tip: get the sturdy stem buds of the hollow plum after step 1) pretreatment, the length is 10mm, strip off the leaves, rinse and carry out routine disinfection, then place the stem buds under a 40-fold dissecting microscope, use Peel off the leaves and leaf primordium with a non-toxic dissecting needle, separate the meristem at the top of the stem bud, the length is 0.5mm, and then quickly inoculate it in...

Embodiment 3

[0050] Embodiment 3 soluble agent

[0051] A method for rapid propagation and detoxification of hollow plum tissue culture, comprising the steps of:

[0052] 1) Selection and pretreatment of implants: Cut off the strong branches on the sandy hollow plum plants, treat them with rooting agents, place the cuttings on the treated artificial substrate, and transfer them to the cultivation room after they survive and grow new shoots. Cultivate for 18 hours at 35°C and 2000Lux light conditions, then culture for 6 hours at room temperature and dark conditions, and co-cultivate for 35 days;

[0053] 2) stripping of the stem tip: get the thick stem buds of the hollow plum after the pretreatment in step 1), the length is 20mm, the leaves are stripped off, rinsed and then routinely sterilized, then the stem buds are placed under a 40-fold dissecting microscope, and the stem buds are used The leaves and leaf primordia are peeled off with a non-toxic dissecting needle, and the meristem at ...

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Abstract

The invention discloses a method for carrying out virus removal on prunus salicina during tissue culture and rapid propagation. The method for carrying out virus removal on prunus salicina during tissue culture and rapid propagation comprises the following steps: 1) selecting an implant and carrying out pre-treatment; 2) peeling stem tips; 3) carrying out induction culture on the stem tips; 4) carrying out multiplication culture; 5) carrying out rooting culture; 6) culturing virus-free seedlings; and 7) detecting viruses and bacteria. The method for carrying out virus removal on prunus salicina during tissue culture and rapid propagation has the advantages that a stem tip virus removal method, a high-temperature treatment virus removal method and a chemical reagent virus removal method are combined, a good virus removal effect in tissue culture and rapid propagation of prunus salicina seedling culture is realized, the obtained virus-free seedlings are subjected to virus detection, and results show that viruses of all the seedlings are successfully removed, and no prune dwarf virus (PDV) or prunus necrotic ringspot virus (PNRSV) infection is found. By utilizing the method disclosed by the invention for carrying out virus removal on prunus salicina, new virus-free prunus salicina seedlings are cultured through tissue culture, and then domestication and transplantation are carried out, so that the aims of increasing the yield and improve the fruit flavour are realized.

Description

technical field [0001] The invention relates to a detoxification method for tissue culture and rapid propagation of hollow plums, and belongs to the technical field of plant tissue culture and rapid propagation and the technical field of plant cell engineering. technical background [0002] Plant virus disease is the main disease in agricultural production, which seriously harms the yield and quality of crops. Once the plant virus disease shows symptoms, it is very difficult to treat. Sand hollow plum is a characteristic fruit in Shazi Town, Yanhe County, Guizhou Province. It has been planted for a long time and is vulnerable to pests and diseases during the cultivation process. Viruses, due to the harm of viral diseases, seriously affect the yield and quality of the fruit, resulting in abnormal fruit shape, reduced taste, reduced quality, and reduced yield. We detected the winter buds collected from the hollow plum seedlings in Shazi Town, Yanhe County, and detected plum d...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00
Inventor 王高平许东东孟彪冯新建
Owner GUIZHOU YANHE WUJIANG BIOTECH DEV
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