A method for automated continuous chromatographic separation of single-walled carbon nanotubes
A technology of single-walled carbon nanotubes and continuous chromatography, which is applied in the field of automatic continuous chromatography to separate single-walled carbon nanotubes, and can solve problems such as clogging of chromatographic columns and failure of chromatographic columns
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Embodiment 1
[0034] A: Configure 1wt% DOC solution as mobile phase.
[0035] B: Weigh 0.0021g of single-walled carbon nanotubes and add them to 10mL of 1wt% DOC aqueous solution for ultrasonic dispersion for 10 hours and high-speed centrifugation for 15 minutes. The supernatant liquid is taken to obtain single-walled carbon nanotube mother liquor.
[0036] C: Gel Chromatographic Separation
[0037] The height of the chromatographic column is 9 cm, the thickness of the soft filter is 2 cm, and the pore size is 800 μm. The agarose gel is used as the chromatographic column filler, and the pore size of the filler is 60 μm.
[0038] Draw 1mL of the single-walled carbon nanotube solution obtained in B, inject it into the packed chromatographic column, use 1wt% DOC as the mobile phase, and pump the flow rate of 0.2mL / min, collect samples every 2min, collect for 1 hour, and obtain the separation single-walled carbon nanotubes.
Embodiment 2
[0040] A: Configure 1.5wt% SDS solution as mobile phase.
[0041] B: Weigh 0.0040g of single-walled carbon nanotubes and add them to 10mL of 1.5wt% SDS aqueous solution for ultrasonic dispersion for 10 hours and high-speed centrifugation for 15 minutes, and take the upper layer of centrifuged liquid to obtain single-walled carbon nanotube mother liquor.
[0042] C: Gel Chromatographic Separation
[0043] The height of the chromatographic column is 15cm, the thickness of the soft filter is 3cm, and the pore size is 1000μm. Sephadex is used as the chromatographic column filler, and the pore size of the filler is 120μm.
[0044] Draw 2mL of the single-walled carbon nanotube solution obtained in B, inject it into the packed chromatographic column, use 1.5wt% SDS as the mobile phase, pump the flow rate of 0.2mL / min, collect samples every 2min, and collect for 1 hour to obtain Isolated single-walled carbon nanotubes.
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