Citrus greening disease rapid detection method

Abstract

The invention discloses a citrus greening disease rapid detection method. The method comprises the following steps: step a, polishing citrus leaves which have been subjected to a dark treatment to get rid of the wax layer on the leaf surfaces, soaking the leaves in a chlorophyll decoloring liquid until the leaves turn white; step b, color development detection: dropwise adding a starch color developing solution on the discolored leaves, and observing the color change of the leaves, if the leaves turn blue, the citrus leaves must be from a citrus tree affected by citrus greening disease, and if the leaves do not change color, the leaves are healthy. The method has the advantages of more convenient operation, shorter time, and more precise result, and can be operated by farmers themselves.

Description

Technical field: [0001] The invention belongs to the field of citrus disease prevention and control, and in particular relates to a rapid detection method for citrus huanglongbing. Background technique: [0002] Citrus huanglongbing disease (HLB) is a destructive disease of citrus that can occur throughout the year, and symptoms can appear in spring, summer and autumn shoots. Generally, there are three types of yellowing of leaves: uniform yellowing, mottled yellowing and deficiency yellowing. In the diseased plants, some new leaves may start to chlorosis and yellowing from the base of the leaves, near the veins or edges, and gradually expand into yellow and green mottled yellowing, and this mottled yellowing may also turn into uniform yellowing; some New shoots are issued on the yellowed branches, some yellowed branches are cut and new shoots are pulled out, the branches are short and the leaves are small and hard, and they also show symptoms of zinc deficiency and mangane...

AI Technical Summary

Problems solved by technology

[0008] (1) Refrigeration is required, although every household has a refrigerator now, some farmers are unwilling to use household refrigerators for freezing citrus leaves;

[0009] (2) The time of freezing and decolorization is long, resulting in too long detection cycle;

[0010] (3) The leaves need to be cut into thin strips of about 1 mm during sample processing, which is difficult for users to grasp during operation

When the thin strip is too wide, the color will be decolorized and the dyeing time will be prolonged, and it will often cause only the cut incision to develop color, which will affect the judgment;

[0011] (4) The test results cannot be released in time, and some farmers hope to see the results in the field without taking them home for experiments;

[0012] (5) The time for freezing and decolorization is too long, it takes 6-10 hours, and when the temperature is low, the time is longer

Images