Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Multifunctional vector capable of carrying out instant positioning of protein

A protein and multifunctional technology, applied in the direction of material inspection products, measuring devices, instruments, etc., can solve the problems of not being able to study the specific functions of natural proteins, not being able to autonomously control the instant positioning of proteins, and fuzzy understanding

Inactive Publication Date: 2014-11-05
常州碳宇纳米科技有限公司
View PDF5 Cites 3 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In current research methods, researchers can only see the subcellular distribution of proteins and cannot autonomously control the instantaneous localization of proteins inside cells
However, the intracellular distribution of proteins is closely related to their functions, and the real-time localization of proteins cannot be effectively controlled, so that we cannot study the specific functions of natural proteins in the original environment. research, even lead to wrong conclusions

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Multifunctional vector capable of carrying out instant positioning of protein
  • Multifunctional vector capable of carrying out instant positioning of protein
  • Multifunctional vector capable of carrying out instant positioning of protein

Examples

Experimental program
Comparison scheme
Effect test

Embodiment Construction

[0045] The present invention will be described in further detail below in conjunction with the examples, and the following examples are explanations of the present invention, but the present invention is not limited to the examples.

[0046] 1. At first use flake graphite (purity 99.95%), prepare graphene according to improved Hummer's method. Then ultrasonically disperse in MilliQ water, filter with a centrifugal filter membrane (100Kd, Millipore), and wash with MilliQ water to obtain ultra-small-sized graphene (size less than 100nm).

[0047] 2. Disperse the above ultra-small size graphene in MilliQ water, add hyperbranched PEI (molecular weight: 25KD) and 6-arm polyethylene glycol (molecular weight per arm: 2000KD), add N-(3-dimethylaminopropyl) -3-Ethylcarbodiimide hydrochloride was stirred at room temperature, filtered with a centrifugal filter membrane (100Kd, Millipore), washed with MilliQ water, and dispersed in MilliQ water to complete the functionalization of PEI and...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

PropertyMeasurementUnit
Sizeaaaaaaaaaa
Sizeaaaaaaaaaa
Login to View More

Abstract

The invention provides a multifunctional vector constructed on the basis of a carbon nanomaterial, which belongs to the field of nano-biotechnology. The vector can enter cells, capture protein and carry out fluorescent tracking and positioning of protein in cells and greatly improves efficiency and accuracy of research on the action mechanism of protein. Application of the multifunctional vector in biotechnology will powerfully promote research on functions and interaction of protein, has an academic theoretical value and can produce immense economic benefits and great social influence. The multifunctional vector is mainly used for research on the functions and action mechanism of protein and is applicable to fields like research on pathogenesis of diseases, drug design and gene therapy.

Description

Technical field: [0001] The invention relates to a multifunctional carrier based on carbon nanomaterials, which can enter cells, capture proteins, perform fluorescence tracking, and perform intracellular protein positioning, thereby improving the efficiency and accuracy of protein action mechanism research. The invention belongs to the field of nano-biotechnology. Background technique: [0002] Nano-biotechnology is a frontier and hot issue in the field of international biotechnology. It has a wide range of applications and clear industrialization prospects in the field of medicine and health. [0003] A protein molecule must be localized to a specific location within a cell to carry out its desired function, so the subcellular distribution of a protein regulates and controls its activity. For example, after a variety of proteins are synthesized, they must be localized to other places other than the cytoplasm to perform their functions, such as the nucleus, cell membrane, a...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
IPC IPC(8): G01N33/68
CPCG01N33/54346G01N33/582G01N33/6845
Inventor 贾凤美刘遵峰刘倩
Owner 常州碳宇纳米科技有限公司
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com