Coding microcarrier material and application thereof

Abstract

The invention relates to a coding microcarrier material and application thereof. The coding microcarrier material is a microcapsule and is of a core-shell structure; the shell layer of the core-shell structure is a hydrogel material with biocompatibility, the core of the core-shell structure contains up-conversion nanoparticles; and the coding microcarrier material can emit a visible light signal under the irradiation of near-infrared exciting light. Compared with the prior art, the coding microcarrier has multiple near-infrared responsiveness anti-Stokes luminescence performance, so that the coding microcarrier has good coding capability; and compared with a traditional coding material, the coding microcarrier has the advantages of good optical and chemical stability, low background signal interference and the like; and a substrate material of the coding microcarrier is the hydrogel with a specific biomolecule recognition function, so that multi-element molecule and cell coding can be realized, and application such as high-throughput biological analysis and detection is facilitated.

Description

technical field [0001] The invention relates to a biomedical application field, in particular to a coded microcarrier material and its application. Background technique [0002] Multivariate analysis technology has very important application value in the fields of biological detection and drug evaluation. Traditional multivariate analysis is mainly based on planar arrays. Probe molecules are fixed at specific positions on the array, and target molecules react specifically with corresponding probe molecules. By reading signals at all sites, the target analyte can be obtained. Related Information. Although this technique has the advantages of high-throughput analysis, however, in the detection reaction, the target molecules need to diffuse to a specific area to react with the probe molecules. [0003] Therefore, overall, the detection efficiency is low. In contrast to this is the liquid chip technology, the core of which is the coded microcarrier. Coded microcarriers reali...

AI Technical Summary

Problems solved by technology

However, fluorescent dyes are prone to photobleaching and there are few types. Under the same excitation light, it is necessary to avoid spectral overlap between multiple dyes, and to avoid resonance energy transfer and mutual quenching, which limit the diversity of coding.

Quantum dot coding is also limited by the mutual overlap of fluorescence intensity or self-toxicity, which is not conducive to obtaining highly sensitive and safe optical coding

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