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Coding microcarrier material and application thereof

A microcarrier and encoding technology, applied in the field of biomedical applications, can solve the problems of fluorescent dyes that are easy to be photobleached, limit the diversity of encoding, and resonant energy transfer, etc., and achieve stable and excellent encoding ability, saving detection costs, and stable luminescence Effect

Pending Publication Date: 2021-11-05
FUDAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, fluorescent dyes are prone to photobleaching and there are few types. Under the same excitation light, it is necessary to avoid spectral overlap between multiple dyes, and to avoid resonance energy transfer and mutual quenching, which limit the diversity of coding.
Quantum dot coding is also limited by the mutual overlap of fluorescence intensity or self-toxicity, which is not conducive to obtaining highly sensitive and safe optical coding

Method used

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  • Coding microcarrier material and application thereof
  • Coding microcarrier material and application thereof
  • Coding microcarrier material and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0048] Immunological detection experiments of microcarriers encoded with upconversion luminescent materials.

[0049] In order to adapt the encoded microcarriers to multiple immunoassays, three single-nuclear upconversion luminescent materials encoding microcarriers that emit red, green, and blue visible light under the excitation of 980nm near-infrared light were mixed with rabbit, human and goat immunoglobulin G (IgG ) protein molecule binding. Because the surface of the encoded microcarrier contains carboxyl groups provided by polyacrylic acid, IgG can be well immobilized on the surface of the microcarrier. After IgG was immobilized, the encoded microcarriers were treated with bovine serum albumin (BSA) to seal any vacancies, thus preventing unwanted adsorption. In the three different encoded microcarrier mixtures treated above, anti-human IgG labeled with fluorescein isothiocyanate (FITC) was introduced, and in the three types of microcarriers, only visible light emitted ...

Embodiment 2

[0051] The upconversion luminescent material encoding microcarrier is used for the detection of various DNA molecules.

[0052] In order to adapt the codable microcarriers to the detection of DNA molecules, the microcarriers encoded by two kinds of single-nuclear upconversion luminescent materials that emit green-blue visible light under the excitation of 980nm near-infrared light were connected to the probe DNA molecule Probe1 by chemical coupling method. and the probe DNA molecule Probe2, while the coded microcarrier that emits red light is used as a control group without any DNA molecule. Afterwards, use the buffer solution to prepare a solution containing 7-amino-4-methylcoumarin (AMC)-labeled target DNA molecule Target1 and target DNA molecule Target2 solution as the sample to be tested, and the emitted light of the fixed probe DNA molecule The blue and green coded microcarriers and the red coded microcarriers of the control group are put into the detection sample togethe...

Embodiment 3

[0054] Up-conversion luminescent material-encoded microcarriers were used for specific capture and isolation experiments of various circulating tumor cells.

[0055] In order to realize the specific capture and separation of various circulating tumor cells by the encoded microcarriers, three single-nuclear up-conversion luminescent materials encoding microcarriers that emit red, green, and blue visible light under the excitation of 980nm near-infrared light were combined with "TD05", "Sgc8", "Sgd5" three DNA aptamer fragments are coupled, and the coupling process is implemented by reacting the carboxyl provided by the polyacrylic acid on the surface of the coding microcarrier with the amino group of the nucleic acid aptamer. Among them, TD05 can specifically bind to the T lymphoma cell line Ramos cells, and Sgc8 can specifically bind to the B lymphoma cell line CCRF-CEM cells. These encoded microcarriers coupled with nucleic acid aptamers were added to the culture medium that ...

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Abstract

The invention relates to a coding microcarrier material and application thereof. The coding microcarrier material is a microcapsule and is of a core-shell structure; the shell layer of the core-shell structure is a hydrogel material with biocompatibility, the core of the core-shell structure contains up-conversion nanoparticles; and the coding microcarrier material can emit a visible light signal under the irradiation of near-infrared exciting light. Compared with the prior art, the coding microcarrier has multiple near-infrared responsiveness anti-Stokes luminescence performance, so that the coding microcarrier has good coding capability; and compared with a traditional coding material, the coding microcarrier has the advantages of good optical and chemical stability, low background signal interference and the like; and a substrate material of the coding microcarrier is the hydrogel with a specific biomolecule recognition function, so that multi-element molecule and cell coding can be realized, and application such as high-throughput biological analysis and detection is facilitated.

Description

technical field [0001] The invention relates to a biomedical application field, in particular to a coded microcarrier material and its application. Background technique [0002] Multivariate analysis technology has very important application value in the fields of biological detection and drug evaluation. Traditional multivariate analysis is mainly based on planar arrays. Probe molecules are fixed at specific positions on the array, and target molecules react specifically with corresponding probe molecules. By reading signals at all sites, the target analyte can be obtained. Related Information. Although this technique has the advantages of high-throughput analysis, however, in the detection reaction, the target molecules need to diffuse to a specific area to react with the probe molecules. [0003] Therefore, overall, the detection efficiency is low. In contrast to this is the liquid chip technology, the core of which is the coded microcarrier. Coded microcarriers reali...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N21/359G01N21/64G01N21/01C12Q1/68G01N33/543G01N33/569G01N33/68
CPCG01N21/359G01N21/6486G01N21/01G01N33/54313C12Q1/68G01N33/68G01N33/56966C12Q2563/149
Inventor 商珞然
Owner FUDAN UNIV
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