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Preparation method of fermenting agent for direct vat set fermented milk

A technology of lactic acid bacteria fermentation and production method, which is applied in the production field of direct-throwing lactic acid bacteria fermented milk starter, can solve the problems of easy product quality problems, unstable passage, low activity of bacterial powder, etc. Probability of microbial contamination, easy process, and the effect of less usage

Inactive Publication Date: 2014-11-19
HANGZHOU WAHAHA TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] After testing, it was found that the bacterial powder of the direct-input fermented milk starter used in production is relatively low, and some are less than 10 10 CFU / g, the dosage that needs to be added in production is too large, and the passage is unstable at the same time, which is prone to product quality problems

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0050] A method for making a starter for direct-throwing lactic acid bacteria fermented milk, comprising the steps of:

[0051] 1. Preparation of Streptococcus thermophilus bacteria powder

[0052] (1) Prepare Streptococcus thermophilus culture medium. The formulation of Streptococcus thermophilus culture medium consists of the following components in weight percentage: lactose 1%, skim milk hydrolyzate 10%, concentrated tomato juice 1%, water-soluble wheat Protein 1%, yeast extract 2%, sterile water 85%. Inoculate Streptococcus thermophilus in Streptococcus thermophilus culture medium for high-density fermentation culture, the inoculation amount is 0.1wt%, during the fermentation process of Streptococcus thermophilus: pH is maintained between 6.6-7.0, and the culture temperature is set at 36.5°C, the fermentation time was set to 5 hours, and ammonia water with a mass concentration of 5% was used to control the fermentation pH during the fermentation process.

[0053] (2) Af...

Embodiment 2

[0068] A method for making a starter for direct-throwing lactic acid bacteria fermented milk, comprising the steps of:

[0069] 1. Preparation of Streptococcus thermophilus bacteria powder

[0070] (1) Prepare the Streptococcus thermophilus culture medium. The formula of the Streptococcus thermophilus culture medium consists of the following components by weight percentage: lactose 9%, skim milk hydrolyzate 1%, concentrated tomato juice 5%, water-soluble wheat Protein 3%, yeast extract 0.1%, sterile water 81.9%. Inoculate Streptococcus thermophilus in Streptococcus thermophilus culture medium for high-density fermentation culture, the inoculation amount is 0.3wt%, during the fermentation process of Streptococcus thermophilus: pH is maintained between 6.6-7.0, and the culture temperature is set at 44.5°C, the fermentation time was set to 4 hours, and 10% ammonia water was used to control the fermentation pH during the fermentation process.

[0071] (2) After the fermentation ...

Embodiment 3

[0087] A method for making a starter for direct-throwing lactic acid bacteria fermented milk, comprising the steps of:

[0088] 1. Preparation of Streptococcus thermophilus bacteria powder

[0089] (1) Prepare Streptococcus thermophilus culture medium. The formula of Streptococcus thermophilus culture medium consists of the following components in weight percentage: lactose 3%, skim milk hydrolyzate 5%, concentrated tomato juice 3%, water-soluble wheat Protein 2%, yeast extract 1%, sterile water 86%. Inoculate Streptococcus thermophilus in Streptococcus thermophilus medium for high-density fermentation culture, the inoculum size is 0.2wt%, during the fermentation process of Streptococcus thermophilus: pH is maintained between 6.6-7.0, and the culture temperature is set at 40°C, the fermentation time was set to 4.5 hours, and 8% ammonia water was used to control the fermentation pH during the fermentation process.

[0090] (2) After the fermentation culture is over, the obtai...

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Abstract

The invention discloses a preparation method of a fermenting agent for direct vat set fermented milk. The preparation method comprises the following steps: preparing streptococcus thermophilus powder; preparing lactobacillus delbrueckii lactobacillus bulgaricus powder; uniformly mixing the streptococcus thermophilus powder and the lactobacillus delbrueckii lactobacillus bulgaricus powder according to the weight ratio of (1.5-2):1; and introducing nitrogen, and packaging to obtain a product. The process disclosed by the invention is simple, easy to operate and suitable for industrialized production; the obtained fermenting agent has the advantages of high active lactobacillus quantity, long quality guarantee period, excellent fermented milk property, easiness for use, small usage amount and the like.

Description

technical field [0001] The invention relates to a preparation method of a leavening agent, in particular to a preparation method of a direct throw-in lactic acid bacteria fermented milk starter. Background technique [0002] In recent years, direct-input fermented milk starter has been widely used in the dairy industry. As a field of biological research, direct-input fermented milk starter has great significance for human nutrition and health. At present, direct-spray fermented milk starter is mainly used in fermented milk products in food, and it is also gradually used in other functional foods, such as juice, ice cream, candy, etc. [0003] In 2012, Euromonitor International, a market research firm, showed that the global sales of fermented foods and supplements similar to direct-injected fermented milk starters amounted to US$28 billion in 2011. One hundred million U.S. dollars. In the global market share of probiotics in 2012, the largest source of income was lactic ac...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C12R1/46C12R1/225
Inventor 侯保朝李言郡李宝磊赵建云葛红娟邢良英马广辉范秋领陈琳陈苏徐显睿陈丽娥
Owner HANGZHOU WAHAHA TECH
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