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A Stable Troponin Detection Kit

A technology for detecting kits and troponin, which is applied in the field of troponin detection kits, can solve problems such as waste, influence on reagent stability, unfavorable reagent long-term storage, etc., and achieve the effect of enhancing stability

Active Publication Date: 2016-11-02
BIOBASE BIODUSTRY (SHANDONG) CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, due to the presence of a large number of latex particles in the troponin detection kit (latex-enhanced immunoturbidimetric method), the stability of the reagent will be affected, which is not conducive to the long-term storage of the reagent, resulting in adverse consequences of waste

Method used

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  • A Stable Troponin Detection Kit
  • A Stable Troponin Detection Kit
  • A Stable Troponin Detection Kit

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0023] Embodiment 1 (comparative example)

[0024] Component 1 (R1):

[0025] Component 1 (R1):

[0026] Glycine buffer pH 7.6

[0027] Sodium chloride 150mmol / L

[0028] Surfactant 3g / L

[0029] Component 2 (R2):

[0030] Glycine buffer pH 7.6

[0031] Goat anti-human TnI antibody coated latex particles 3g / L.

[0032] When using, use a fully automatic biochemical analyzer with dual reagent functions, such as Toshiba 40 fully automatic analyzer, etc., and use the dual reagent endpoint method for determination. Place R1 and R2 on the corresponding reagent positions according to the ratio of 3:1, and place distilled water, standards and samples on the corresponding positions of the sample plate. The operation is shown in Table 1:

[0033] Table 1 Detection method of troponin I detection reagent

[0034]

[0035] Calculation: troponin I concentration = (∆A determination ÷ ∆A standard) × C standard

Embodiment 2

[0037] Glycine buffer pH 7.6

[0038] Sodium chloride 150mmol / L

[0039] Alkyl glucoside 3g / L;

[0040] Component 2 (R2):

[0041] MOPOS buffer 25mmol / L

[0042] Goat anti-human TnI antibody coated latex particles 3g / L.

[0043] The method of use is the same as above.

Embodiment 3

[0045] Glycine buffer pH 7.6

[0046] Sodium chloride 250mmol / L

[0047] Alkyl glucoside 3g / L;

[0048] Component 2 (R2):

[0049] MOPOS buffer 50mmol / L

[0050] Goat anti-human TnI antibody coated latex particles 3g / L.

[0051] The method of use is the same as above.

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Abstract

The invention relates to a troponin detection kit with strong stability. The troponin detection kit includes a component 1 and a component 2. The components include the following raw materials and contents: the component 1 comprising a glycine buffer solution with pH of 7.6, 150-250 mmol / L of sodium chloride and 3 g / L of alkyl polyglycoside; and the component 2 comprising 25-50 mmol / L of an MOPOS buffer solution and 3 g / L of goat anti-human TnI antibody coated latex particles. The stability of the reagents provided by the invention is obviously higher than that of conventional similar reagents.

Description

technical field [0001] The invention relates to a highly stable troponin detection kit, which belongs to the technical field of clinical in vitro detection reagents. Background technique [0002] Troponin, composed of three subunits T, C, and I, together with tropomyosin regulates the interaction between actin and myosin by regulating the activity of calcium ions on striated actin ATPase. Cardiac troponin I (cTnI) has a molecular weight of 22.5 KD and together with troponin T and troponin C form a structural complex. Plays an important role in the translation of actin-myosin interactions in intercellular calcium signaling in the heart. Human cTnI differs structurally from the skeletal muscle form by the addition of an amino acid residue at the amino terminus. Make cTnI a specific marker. After myocardial infarction (AMI), cTnI is rapidly released into the blood circulation. The pattern of release was the same as for CK-MB (4-6 hours after AMI). However, CK-MB levels ret...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N33/68
CPCG01N33/54393G01N2333/4712G01N2800/324
Inventor 甘宜梧董雯谭柏清王绮李静
Owner BIOBASE BIODUSTRY (SHANDONG) CO LTD