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Heterozygous genome processing method

A processing method and genome technology, applied in the fields of genetic engineering and genome bioinformatics analysis, can solve the problems of high cost, long cycle, restricting species genome interpretation, etc., and achieve the effect of reducing cost and shortening cycle.

Active Publication Date: 2017-09-19
BGI TECH SOLUTIONS
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Problems solved by technology

[0004] The technical problem to be solved by the present invention is to provide a hybrid genome processing method in view of the defects of the traditional hybrid genome map drawing scheme in the prior art that the cost is high and the cycle is long, which seriously restricts the interpretation of the species genome

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Embodiment Construction

[0057] The objects, features and characteristics of the present invention, as well as the operation method of the relevant elements of the structure and the function and combination of parts will become more apparent when reading the following description and the above claims with reference to the accompanying drawings. It is to be expressly understood that the drawings are for purposes of illustration and description only and are not intended as limitations of the invention.

[0058] In the heterozygous genome processing method of the present invention, without any reference sequence, based on the next-generation high-throughput sequencing technology, the target species is sequenced at an ultra-high depth (generally more than 200 times), and then the new version of SOAPdenovo2 assembly software is used , combined with heterozygous recognition processing, to obtain the whole genome sequence map of the target species. Embodiments of the present invention may include whole genome ...

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Abstract

The invention discloses a heterozygous genome processing method, which includes performing at least 200-fold ultra-high-depth sequencing on the target species by using the whole genome shotgun method to obtain effective read length and short sequence Reads; performing assembly and processing on the effective Reads Construct the scaffold sequence Scaffold to obtain a genome map with redundant sequences; perform heterozygous identification processing on the genome map with redundant sequences, thereby removing redundant Scaffold in the heterozygous region and retaining the heterozygous region information for Obtain a genome-wide map. Through the method of the invention, a complete and high-quality heterozygous genome map can be quickly assembled, and the drawing cycle and cost are shortened.

Description

technical field [0001] The invention relates to the technical field of genetic engineering and the field of genome bioinformatics analysis. In particular, the invention relates to heterozygous genome processing to obtain a whole genome sequence map of a species. Background technique [0002] Whole-genome de novo sequencing is also called de novo sequencing. A species can be sequenced without any reference sequence, and the whole genome sequence map of the species can be obtained by splicing and assembling using bioinformatics analysis methods. Obtaining the full genome sequence of a species is an important shortcut to speeding up our understanding of that species. [0003] With the launch of the Human Genome Project, the world has taken an important step in genome sequencing; various models of animal and plant nematodes, Arabidopsis, mice, etc. have been sequenced genomes; due to technical limitations, genome sequencing is still in its infancy. After the completion of the ...

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12Q1/68G16B30/20G16B20/20
CPCC12Q1/6869G16B30/20G16B20/20C12Q2537/165G16B20/00G16B30/00
Inventor 詹东亮
Owner BGI TECH SOLUTIONS
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