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A kind of natural biological bone material and preparation method thereof

A technology of bone material and product, applied in the field of natural biological bone material and its preparation, can solve the problems of losing the function of filling and protection, inhibiting the normal growth of repaired tissue, unable to synchronize the speed of tissue repair, and achieving permeability that is beneficial to Effect

Active Publication Date: 2016-02-17
YANTAI ZHENGHAI BIO TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Natural materials such as collagen sponge degrade too fast in the body and cannot synchronize with the repair speed of the tissue. Often the damaged site has not been fully repaired but the collagen sponge has been completely degraded, thus losing the role of filling protection, and the degradation products of polylactic acid It will cause local strong acidity and inhibit the normal growth of the repaired tissue

Method used

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  • A kind of natural biological bone material and preparation method thereof
  • A kind of natural biological bone material and preparation method thereof
  • A kind of natural biological bone material and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0027] Embodiment 1, the preparation of natural biological bone material

[0028] 1. Preparation of spongy bone

[0029] (1) Collect leg bones of cows that have just been slaughtered from slaughterhouses under standardized management, avoid contact with pollutants as much as possible, and freeze them immediately after collection.

[0030] (2) The leg bone obtained in step (1) was thawed and fully cleaned, the cancellous bone was cut out, and then the cancellous bone was cut into an easy-to-handle shape, and the surface attachments were washed away.

[0031] 2. Preparation of natural biological bone materials

[0032] (1) The first surfactant treatment

[0033] Treat spongy bone with a surfactant solution soak.

[0034] The purpose of this step is to destroy the cell membrane structure and break up and dissolve the cells.

[0035] Specific steps: soak in 0.5g / 100ml TritonX-100 aqueous solution at 8±2°C for 12 hours, and then wash with water.

[0036] (2) The first alkali t...

Embodiment 2

[0055] Embodiment 2, the preparation of natural biological bone material

[0056] 1. Preparation of spongy bone

[0057] (1) Collect leg bones of freshly slaughtered pigs from slaughterhouses under standardized management, avoid contact with pollutants as much as possible, and store them in frozen storage immediately after collection.

[0058] (2) The leg bone obtained in step (1) was thawed and fully cleaned, the cancellous bone was cut out, and then the cancellous bone was cut into an easy-to-handle shape, and the surface attachments were washed away.

[0059] 2. Preparation of natural biological bone materials

[0060] (1) The first surfactant treatment

[0061] Treat spongy bone with a surfactant solution soak.

[0062] Specific steps: Soak with 1g / 100ml Tween-80 aqueous solution at 23±2°C for 5 hours, then wash with water.

[0063] (2) The first alkali treatment

[0064] The product of step (1) is soaked in an alkaline solution.

[0065] Specific steps: use 0.5M KOH...

Embodiment 3

[0078] Embodiment 3, the preparation of natural biological bone material

[0079] 1. Preparation of spongy bone

[0080] (1) Collect leg bones of cows that have just been slaughtered from slaughterhouses under standardized management, avoid contact with pollutants as much as possible, and freeze them immediately after collection.

[0081] (2) The leg bone obtained in step (1) was thawed and fully cleaned, the cancellous bone was cut out, and then the cancellous bone was cut into an easy-to-handle shape, and the surface attachments were washed away.

[0082] 2. Preparation of natural biological bone materials

[0083] (1) The first surfactant treatment

[0084] Treat spongy bone with a surfactant solution soak.

[0085] Specific steps: Soak in 3g / 100ml Tween-40 aqueous solution at 2±2°C for 168 hours, then wash with water.

[0086] (2) The first alkali treatment

[0087] The product of step (1) is soaked in an alkaline solution.

[0088] Specific steps: use 0.02MCa(OH) 2...

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Abstract

The invention discloses a natural biological bone material and its preparation method. The method for preparing the natural biological bone material comprises the following steps: (1) a surfactant solution is used to immerse and process isolated cancellous bone; (2) aqueous alkali is used to immerse and process a product obtained from the step (1); (3) a surfactant solution is used to immerse and process a product obtained from the step (2); (4) aqueous alkali is used to immerse and process a product obtained from the step (3); (5) a PBS buffer solution is used to immerse and process a product obtained from the step (4); and (6) a product obtained from the step (5) undergoes freeze drying and irradiation sterilization successively so as to obtain the natural bone material. The natural bone material provided by the invention can completely be degraded in the body, and degradation can be synchronous with tissue repair.

Description

technical field [0001] The invention belongs to the field of medical instruments, and in particular relates to a natural biological bone material used for repairing bone tissue or organs in surgery and a preparation method thereof. Background technique [0002] Modern surgical operations often need to repair or repair some defective bone tissue or bone organs, such as repair of skull defects, dental implants, etc. [0003] After people enter middle age, the bone repair ability is limited, and the self-repair of bone tissue or bone organs takes a long time. During the repair process, the damaged part is easily invaded by the surrounding muscle tissue and connective tissue, which eventually leads to incomplete bone repair and affects the normal function of the human body. Therefore, it is necessary to fill some materials in the damaged part during the bone repair process to prevent the invasion of peripheral tissues. [0004] In order to meet the needs of surgical operations...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61L27/36A61L27/12A61L27/24A61L27/54A61L27/58
Inventor 孙先昌林萍郭松董佳桓蒋绍霞
Owner YANTAI ZHENGHAI BIO TECH