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Kit and method for rapidly extracting plant dna using dna adsorption column

An adsorption column and kit technology, applied in the field of kits for rapid extraction of plant DNA, can solve the problems of loss, easy degradation, poor integrity of DNA samples, etc., and achieve the effect of good integrity and high purity

Inactive Publication Date: 2017-06-30
FUJIAN NORMAL UNIV
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The traditional plant tissue DNA extraction process is time-consuming and prone to DNA loss and degradation.
[0004] Therefore, it is necessary to develop a convenient, fast and practical method suitable for plant genomic DNA extraction, so as to solve the shortcomings of DNA samples obtained by conventional methods such as poor integrity and easy degradation.

Method used

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  • Kit and method for rapidly extracting plant dna using dna adsorption column
  • Kit and method for rapidly extracting plant dna using dna adsorption column

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Experimental program
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Effect test

Embodiment Construction

[0025] A kit and method for rapidly extracting plant DNA using a DNA adsorption column, the specific steps are as follows:

[0026] (1) Raw materials: Weigh 1 g of each of the five plant samples of rice, sugarcane, corn, peanut, and wheat.

[0027] (2) Pretreatment of plant tissue samples: Grind the weighed rice, sugarcane, corn, peanut, and wheat tissues into powder with liquid nitrogen.

[0028] (3) Extraction of free DNA and impurity proteins: put the sample powder into a 2ml centrifuge tube, add 800μl lysate, shake the oscillator for 30 seconds, incubate in a 65°C water bath for 30 minutes, every 5 minutes Shake once. After incubation, 800 μl of extract was added, shaken for 30 seconds, and then centrifuged at 12000 rpm / min for 10 minutes. Transfer the supernatant to a new centrifuge tube.

[0029] (4) Adsorption of DNA by DNA adsorption column and removal of impurities: add an equal volume of precipitant to the centrifuge tube containing the supernatant, mix well and a...

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Abstract

The invention relates to a kit and a method for quickly extracting plant DNA by using a DNA adsorption column, belonging to the field of biotechnology. The composition of the kit includes a lysate, an extraction solution, a precipitation agent, a protein-removing solution, a washing solution, an eluent and a DNA adsorption column. The method includes the pretreatment of plant tissue samples, the extraction of free DNA and impurity proteins, the adsorption of DNA by a DNA adsorption column and the removal of impurities, and the elution of DNA. The plant genomic DNA can be rapidly extracted by using the kit, and the obtained genomic DNA has high purity.

Description

technical field [0001] The invention relates to a kit and a method for quickly extracting plant DNA by using a DNA adsorption column, belonging to the field of biotechnology. Background technique [0002] DNA extraction is the basic technology of plant molecular biology research. The continuous development of DNA extraction technology has created favorable conditions for many applications such as whole genome sequencing. With the development of modern molecular biology, DNA isolation techniques emerge in endlessly. DNA can already be isolated from extreme materials such as fossils, mummies, etc., and can also be obtained from trace materials. At present, a variety of methods have been developed to successfully extract DNA from plant leaves, callus, tissue culture seedlings, fruits, phloem and other tissues and organs. [0003] Traditional DNA extraction and purification, such as CTAB method and SDS method, are based on lysed cells, repeated extractions with organic solven...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N15/10
Inventor 王清水余彦
Owner FUJIAN NORMAL UNIV