Salt resistance gene msvp2 of energy crop hag and its plant expression vector and construction method

A technology for plant expression vector and salt tolerance gene, applied in the field of molecular biology, can solve the problems of limited salt tolerance, low efficiency, affecting cultivation and application, etc., and achieve the effect of improving salt tolerance and variety improvement

Active Publication Date: 2017-01-11
INST OF BOTANY JIANGSU PROVINCE & CHINESE ACADEMY OF SCI
View PDF1 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Grass (Miscanthus sacchariflora) is a plant of the genus Miscanthus Anderss. in the Gramineae family. As an important energy plant native to my country, it has high development and utilization value. ) and other materials have relatively limited salt tolerance, which greatly affects their cultivation and application in saline-alkali land
Given that salt tolerance is a quantitative trait controlled by multiple genes, conventional breeding methods such as cross-breeding are inefficient

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Salt resistance gene msvp2 of energy crop hag and its plant expression vector and construction method
  • Salt resistance gene msvp2 of energy crop hag and its plant expression vector and construction method
  • Salt resistance gene msvp2 of energy crop hag and its plant expression vector and construction method

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0034] Example 1 Construction and Transformation of Plant Expression Vector pEarleyGate103-MsVP2

[0035] 1. Cloning of MsVP2:

[0036] Grass (Miscanthus sacchariflora) was selected as the material, and healthy cuttings were selected, and the seedlings (20 days) that survived the cuttings were continuously stressed with salt (200mM NaCl) for 3 days, and 0.05g of young leaves were taken, and were extracted with reference to the Trizol RNA extraction kit (TaKaRa ) method described in the instructions, extracting total RNA from leaves, taking 1 μg of total RNA to reverse transcribe into cDNA according to the M-MLV Reverse Transcription Kit (TaKaRa), digesting the cDNA product with RNase, referring to the Arabidopsis VP2 sequence information (NCBI accession number: AT1G16780 ), analyzed and designed primers to amplify MsVP2 by Oligo6.0 software;

[0037] Upstream primer MsVP2-F: 5′-ATGATGGAAGAAGACGTGGA-3′

[0038] Downstream primer MsVP2-R: 5′-CAAGAATATTGGTGCCATGACC-3′

[0039]...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

No PUM Login to view more

Abstract

The invention belongs to the field of molecular biology, and relates to a silvergrass salt-tolerant gene MsVP2 as well as an expression vector and a construction method thereof. The sequence of the MsVP2 gene is SEQ ID NO.1; and the constructed expression vector pEarleyGate103-MsVP2 is obtained by inserting the MsVP2 gene into a cloning vector pMD10-T simple vector, performing double digestion on the MsVP2 gene by use of Sal I and Not I and then connecting the MsVP2 gene to Sal I and Not I sites of a gateway entry vector pENTR1A, linearizing a pENTR1A-MsVP2 plasmid and then performing LR recombination reaction on the linearized pENTR1A-MsVP2 plasmid and a pEarleyGate103 vector plasmid. The silvergrass MsVP2 provided by the invention is a new salt-tolerant gene, can be used for enhancing the salt resistance of plants and can be applied to new salt-tolerant idioplasm creation and plant breed improvement.

Description

technical field [0001] The invention belongs to the field of molecular biology, and relates to a grass salt-tolerant gene MsVP2, a plant expression vector and a construction method thereof. Background technique [0002] Salt stress is an important stress factor affecting plant growth and development, and a key factor limiting the efficient use of saline-alkali land in my country. Therefore, the study of salt tolerance has become a hot field. Grass (Miscanthus sacchariflora) is a plant of the Gramineae genus Miscanthus Anderss. As an important energy plant native to my country, it has high development and utilization value. ) and other materials have relatively limited salt tolerance, which greatly affects their cultivation and application in saline-alkali land. Given that salt tolerance is a quantitative trait controlled by multiple genes, conventional breeding methods such as cross-breeding are inefficient. Existing studies have shown that the molecular breeding of high-e...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
Patent Type & Authority Patents(China)
IPC IPC(8): C12N15/55C12N9/14C12N15/66C12N15/82A01H5/00
Inventor 宗俊勤刘建秀陈煜陈静波李兰兰郭爱桂
Owner INST OF BOTANY JIANGSU PROVINCE & CHINESE ACADEMY OF SCI
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap