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Method for Fermenting Soybean Meal with Composite Bacteria Liquid

A technology for fermenting soybean meal and compound bacterial liquid, which is applied in the fields of fermentation and food science, and can solve the problems of low nutritional value and incomplete fermentation of soybean meal.

Active Publication Date: 2016-07-06
WEIHAI GOLD FEED
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The purpose of the present invention is to solve the technical problems of incomplete fermentation of soybean meal and low nutritional value in the existing soybean meal fermentation method, and to provide a method for fermenting soybean meal with a compound bacterial liquid that has complete fermentation and high nutritional value

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0023] First prepare the Bacillus natto liquid, the Bacillus coagulans liquid and the Lactobacillus liquid, and the specific preparation process is as follows:

[0024] (1) Preparation of Bacillus natto liquid

[0025] Inoculate the Bacillus natto strain into the nutrient agar medium, inoculate it on the nutrient agar slant after 12 hours of activation culture, inoculate it in the shake flask after 20 hours of slant culture 8 For the seed liquid of CFU / ml, the seed liquid was inoculated into a 100L seed tank with a 5% inoculation amount, transplanted into a 1T fermenter after 12 hours of cultivation, and transplanted into a 10T fermenter after 14 hours of cultivation, and cultivated for 24 hours. The number of live bacteria obtained in hours is 2.0×10 8 CFU / ml of Bacillus natto liquid.

[0026] The seed liquid is inoculated into the 100L seed tank with 5% inoculation amount, which means that the liquid filling amount of the fermenter is 70% of the volume of the fermenter, an...

Embodiment 2

[0035] First prepare the Bacillus natto liquid, the Bacillus coagulans liquid and the Lactobacillus liquid, and the specific preparation process is as follows:

[0036] (1) Preparation of Bacillus natto liquid

[0037] Inoculate the Bacillus natto strain into the nutrient agar medium, inoculate it on the nutrient agar slant after 16 hours of activation culture, inoculate it in the shake flask after 25 hours of slant culture 8CFU / ml seed liquid, inoculate the seed liquid into a 100L seed tank with 8% inoculum, transplant it into a 1T fermenter after 14 hours of cultivation, and transfer it to a 10T fermenter after 16 hours of cultivation, and cultivate it for 30 hours The number of live bacteria obtained was 3.0×10 8 CFU / ml of Bacillus natto liquid.

[0038] The seed liquid is inoculated into the 100L seed tank with 8% inoculum amount, which means that the liquid filling amount of the fermenter is 70% of the fermenter volume, and the inoculum amount refers to 8% of this 70%, ...

Embodiment 3

[0047] First prepare the Bacillus natto liquid, the Bacillus coagulans liquid and the Lactobacillus liquid, and the specific preparation process is as follows:

[0048] (1) Preparation of Bacillus natto liquid

[0049] Inoculate Bacillus natto strains into nutrient agar medium, inoculate on nutrient agar slant after 20 hours of activation culture, inoculate in shake flask after 30 hours of slant culture 9 CFU / ml seed liquid, inoculate the seed liquid into a 100L seed tank with 10% inoculum, transplant it into a 1T fermenter after 16 hours of cultivation, transplant it into a 10T fermenter after 18 hours of cultivation, and cultivate it for 36 hours to obtain The number of live bacteria is 2.0×10 9 CFU / ml Bacillus natto liquid.

[0050] The seed liquid is inoculated into the 100L seed tank with 10% inoculation amount, which means that the liquid filling amount of the fermenter is 70% of the volume of the fermenter, and the inoculation amount refers to 10% of this 70%, the sam...

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Abstract

The invention relates to a method for fermenting soybean meal with a compound bacterial liquid, which solves the technical problems of incomplete fermentation of soybean meal and low nutritional value in the existing soybean meal fermentation method, and the bacterial liquid obtained by mixing Bacillus natto and Bacillus coagulans It is connected to the expanded soybean meal, mixed with aerobic fermentation; then, the lactobacillus is inserted into the fermented product, and after mixing, it is anaerobically fermented to obtain the fermented soybean meal. The present invention can be widely used in the field of soybean meal fermentation.

Description

technical field [0001] The invention relates to a method for fermenting soybean dregs, in particular to a method for fermenting soybean dregs with a compound bacterial solution. Background technique [0002] Soybean meal is one of the most important vegetable protein sources for livestock and poultry, and its crude protein content is as high as 43-48%. As a high-quality bio-fermented protein raw material, fermented soybean meal efficiently utilizes plant-derived protein to solve the problems of digestion and absorption of anti-nutritional factors and palatability. It can partially or completely replace animal protein raw materials in animal feed, and has rich sources and stable quality. , easy to preserve and other characteristics, it has been widely used in animal feed production. [0003] However, in the process of solid-state fermentation of soybean meal with complex bacteria, the method of simultaneous inoculation of multiple bacteria cannot be well grasped and controll...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12P1/02
Inventor 李贵杰王燕张群峰耿建
Owner WEIHAI GOLD FEED
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