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Fluorescent nanosphere as well as preparation method and application thereof

A fluorescent nanometer and reaction temperature technology, applied in the direction of fluorescence/phosphorescence, chemical instruments and methods, luminescent materials, etc., can solve the problems of no fluorescence, narrow application range, etc., and achieve good fluorescence, simple structure, and easy-to-obtain raw materials Effect

Inactive Publication Date: 2014-12-10
南京博炫生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0013] However, although the research on nanomaterials has been booming, there are also its shortcomings, just like C balls, although it is a breakthrough to arrange the products into an orderly spherical shape, its application range is narrow and it has no fluorescence. Also not good for bioassays

Method used

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  • Fluorescent nanosphere as well as preparation method and application thereof
  • Fluorescent nanosphere as well as preparation method and application thereof
  • Fluorescent nanosphere as well as preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0054] When compound I is When n=50~100, compound II is C 50-100 In the case of COOH, the fluorescent nanospheres contain a mixture of compounds of the following general formula IV:

[0055]

[0056] 1) React fluorescent compound I and 1,3-propanediamine to prepare compound intermediate III; the reaction temperature is 10°C, the reaction time is 48 hours, and the reaction solvent is selected from: dichloromethane, chloroform, compounds I and 1, The molar ratio of 3-propanediamine is 1:3;

[0057] 2) Compound IV is obtained by condensing intermediate III and compound II; the reaction temperature is 10-200° C., and the reaction time is 1-48 hours to obtain fluorescent nanospheres. The reaction solvent is selected from: dichloromethane, chloroform, intermediate III and The molar ratio of compound II is 1:3.

Embodiment 2

[0059] When compound I is When n=100~200, compound II is C 100-200 In the case of COOH, the fluorescent nanospheres contain a mixture of compounds of the following general formula IV:

[0060]

[0061] 1) React fluorescent compound I and 1,3-propanediamine to prepare compound intermediate III; the reaction temperature is 100°C, the reaction time is 24 hours, and the reaction solvent is selected from: ethanol, acetonitrile, compound I and 1,3- The molar ratio of propylenediamine is 1:1;

[0062] 2) Compound IV is obtained by condensing intermediate III with compound II; the reaction temperature is 100° C., and the reaction time is 24 hours. The reaction solvent is selected from: dichloromethane, chloroform, ethanol, acetonitrile, ethyl acetate, toluene, di The molar ratio of toluene, intermediate III and compound II is 1:1.

Embodiment 3

[0064] When compound I is When n=100~200, compound II is C 100-200 In the case of COOH, the fluorescent nanospheres contain a mixture of compounds of the following general formula IV:

[0065]

[0066] 1) React fluorescent compound I and 1,3-propylenediamine to prepare compound intermediate III; the reaction temperature is 200°C, the reaction time is 1 hour, and the reaction solvent is selected from: ethyl acetate, toluene, xylene, compound I The molar ratio with 1,3-propanediamine is 1:5;

[0067] 2) Condensing intermediate III with compound II to obtain compound IV; the reaction temperature is 200° C., the reaction time is 1 hour, the reaction solvent is selected from: ethyl acetate, toluene, xylene, and the molar ratio of intermediate III to compound II is 1:5.

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Abstract

The invention discloses a fluorescent nanosphere which contains a compound with the structural formula shown in the general formula IV (in the Specification), R in the formula IV adopts a structural formula shown in the Specification or another structural formula shown in the Specification, R' is Cn, and the n is 20-200. The invention also discloses a method for preparing the fluorescent nanosphere. The invention further discloses application of the fluorescent nanosphere. According to the invention, a compound II (C sphere) is introduced into a fluorescent functional group in a groundbreaking manner, so that the fluorescent nanosphere can be better applied to biological detection; aiming at the defects in the prior art, through improvement on this basis, the invention provides the preparation method for the combination of the C sphere and the fluorescent compound; the fluorescent nanosphere disclosed by the invention has good fluorescence, the structure is relatively simple, raw materials are easy to obtain, the toxicity is low, a target molecule can be synthesized by 2-3 steps of reaction generally, and the industrialization is easy.

Description

technical field [0001] The invention belongs to the technical field of fine chemicals, and in particular relates to a preparation method and application of fluorescent nanospheres. It is mainly the preparation method of introducing 5(6) carboxyfluorescein or cyanine fluorescent compound into the C ball and its application in biological dyeing. Background technique [0002] 1. Advances in chemical synthesis of nanostructures [0003] Discovery of carbon nanotubes [1] The research and application of low-dimensional nanostructures has opened up a new direction. With the deepening of research, various novel one-dimensional nanostructures, such as non-carbon nanotubes [2] ,Nano stave [3] ,Nanowires [4] nano coaxial cable [5] Following its discovery, it aroused widespread international concern. [0004] According to the spatial dimension, the basic units of nanomaterials can be divided into three categories: zero-dimensional (0D), one-dimensional (1D) and two-dimensional (2...

Claims

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Application Information

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IPC IPC(8): C09K11/06C07D493/10G01N21/64
Inventor 顾玉龙孔向阳李宋敖马飞飞
Owner 南京博炫生物科技有限公司
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