Human serum CXCL16 enzyme-linked immunosorbent assay kit as well as preparation and use methods thereof

Abstract

The invention provides a human serum CXCL16 enzyme-linked immunosorbent assay kit as well as preparation and use methods thereof. The human serum CXCL16 enzyme-linked immunosorbent assay kit comprises an enzyme-labeled reaction plate, a CXCL16 standard product, biotin-labeled antihuman CXCL16 antibodies and streptavidin-labeled horse radish peroxidase, wherein the enzyme-labeled reaction plate is coated with goat-anti-human CXCL16 monoclonal antibodies. The preparation method of the human serum CXCL16 enzyme-linked immunosorbent assay kit comprises the steps of preparing the enzyme-labeled reaction plate, the CXCL16 standard product, the biotin-labeled antihuman CXCL16 antibodies and streptavidin-labeled horse radish peroxidase, wherein the enzyme-labeled reaction plate is coated with the goat-anti-human CXCL16 monoclonal antibodies. The use method of the human serum CXCL16 enzyme-linked immunosorbent assay kit comprises the steps of adding samples, biotinylation antibodies and horse radish peroxidase, and carrying out color developing and terminating.

Description

technical field [0001] The invention relates to the field of immunological detection, in particular to an enzyme-linked immunoassay kit for human serum CXCL16 and a preparation and use method thereof. Background technique [0002] Chemokines (CK) are small molecular proteins with various biological functions and are very important members of the cytokine family. There are four conserved cysteines (C) in all CK molecules. According to the positions of the first two cysteine ​​amino acids at the amino terminal (N-terminal), chemokines are divided into four categories: C, CC, CXC and CX3C class, the corresponding receptors are called CR, CCR, CXCR and CX3CR. At present, the chemokines discovered mainly belong to the CXC class and the CC class. As control targets, chemokines and their receptor molecules can be used for the control and treatment of related diseases. [0003] CXCL16 is a unique CXC chemokine that exists in both transmodel and soluble forms. The full-length CXC...

AI Technical Summary

Problems solved by technology

At present, there are ELISA kits for the determination of CXCL16 in foreign countries, but most of them are only for scientific research applications, and are not suitable for clinical use due to factors such as time-consuming operation, such as the Immunoassay DCX160 series of R&D Systems

However, domestic ELISA kits for the determination of CXCL16 are moderately priced, but the quality of the kits is not stable, and the sensitivity and accuracy cannot meet the requirements of clinical applications.

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