Tissue cultivation method for elegant-purple clematis

A tissue culture and clematis technology, applied in the field of reproduction, can solve the problems affecting the industrialization development of fine clematis varieties, low seed reproduction rate of clematis, low rooting rate of cutting propagation, etc., and achieves easy rooting and reproduction cycle. Short, new and slightly growing good effect

Active Publication Date: 2014-12-17
JIANGSU POLYTECHNIC COLLEGE OF AGRI & FORESTRY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Clematis seed reproduction rate is very low, cutting propagation rooting rate is not high, and using tissue culture and somatic embryogenesis is a very promising approach. Many plants (such as cyclamen) use tissue culture to obtain lar

Method used

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  • Tissue cultivation method for elegant-purple clematis
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  • Tissue cultivation method for elegant-purple clematis

Examples

Experimental program
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Effect test

Embodiment 1

[0035] A clematis tissue culture method, is characterized in that, comprises the following steps:

[0036] (1) Obtain sterile vaccine materials:

[0037] Cut the newly germinated stems with buds of clematis in spring, wash them under tap water for 30 minutes, put them on the ultra-clean workbench, soak them in ethanol with a volume concentration of 70% for 15 seconds, and then soak them in 1% (mass concentration) mercury chloride solution After 8 minutes, rinse 5 times with sterile water, and dry the surface moisture of the stem section with buds;

[0038] (2) Primary culture:

[0039] Get the stem section with buds after the above-mentioned aseptic treatment, cut into 1cm long sections, each section has at least one well-developed, healthy bud without damage from diseases and insect pests, and inoculate it on the induction medium with a pH value of 5.8, The stem segment with buds begins to produce clustered buds 7 days after the induction culture in the induction medium, an...

Embodiment 2

[0044] Embodiment 2: a kind of Clematis tissue culture method is characterized in that, comprises the following steps:

[0045] (1) Obtain sterile vaccine materials:

[0046] Cut the newly germinated stems with buds of clematis in spring, wash them under tap water for 60 minutes, soak them in 75% ethanol (volume concentration) for 20 seconds on the ultra-clean workbench, and then use 1% (mass concentration) mercury chloride After soaking in the solution for 15 minutes, rinse with sterile water for 8 times, and dry the surface moisture of the stem section with buds;

[0047] (2) Primary culture:

[0048] Get the stem section with buds after the above-mentioned aseptic treatment, cut into 1cm long sections, each section has at least one well-developed, healthy bud without damage from diseases and insect pests, and inoculate it on the induction medium with a pH value of 5.6, The stem segment with buds begins to produce clustered buds 7 days after the induction culture in the in...

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Abstract

The invention discloses a tissue cultivation method for elegant-purple clematis. The method comprises the steps of: (1) obtaining an aseptic seeding material; (2) performing primary culture; (3) performing enrichment culture; and (4) performing rooting and seedling strengthening culture; when the root length reaches 2-4cm, acclimating and transplanting can be performed. The tissue cultivation method for elegant-purple clematis provided by the invention has the advantages that, propagation cycle is short, propagation coefficient is high, roots are easy to grow, root systems of plants are thick and strong, growth vigor of new shoots is good, stalks are thick and strong, and acclimated seedling is easy to survive.

Description

technical field [0001] The invention relates to a method for assembling garden plants, in particular to a method for tissue culture of clematis, which belongs to the technical field of reproduction. Background technique [0002] Clematis belongs to the genus Clematis L. of the family Ranunculaceae (clematis fusca var. violacea), and is a class of vines with high ornamental value and a variety of stress resistance. Clematis are perennial woody or herbaceous vines, and a few are erect shrubs. The leaves are opposite and have stalks. Leaves simple, triple or pinnately compound, entire, serrated or divided. Inflorescences cymes, panicles or solitary. Clematis plants have novel and unique flower shapes, large changes, harmonious flower colors, and unique style; they have a long flowering period, strong adaptability, and strong cold resistance. They occupy an important position in international ornamental horticulture. Today, in the pursuit of new, strange and special flower v...

Claims

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Application Information

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IPC IPC(8): A01H4/00
Inventor 宋微王磊张虎潘静霞
Owner JIANGSU POLYTECHNIC COLLEGE OF AGRI & FORESTRY
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