Tissue cultivation method for elegant-purple clematis
A tissue culture and clematis technology, applied in the field of reproduction, can solve the problems affecting the industrialization development of fine clematis varieties, low seed reproduction rate of clematis, low rooting rate of cutting propagation, etc., and achieves easy rooting and reproduction cycle. Short, new and slightly growing good effect
- Summary
- Abstract
- Description
- Claims
- Application Information
AI Technical Summary
Problems solved by technology
Method used
Image
Examples
Embodiment 1
[0035] A clematis tissue culture method, is characterized in that, comprises the following steps:
[0036] (1) Obtain sterile vaccine materials:
[0037] Cut the newly germinated stems with buds of clematis in spring, wash them under tap water for 30 minutes, put them on the ultra-clean workbench, soak them in ethanol with a volume concentration of 70% for 15 seconds, and then soak them in 1% (mass concentration) mercury chloride solution After 8 minutes, rinse 5 times with sterile water, and dry the surface moisture of the stem section with buds;
[0038] (2) Primary culture:
[0039] Get the stem section with buds after the above-mentioned aseptic treatment, cut into 1cm long sections, each section has at least one well-developed, healthy bud without damage from diseases and insect pests, and inoculate it on the induction medium with a pH value of 5.8, The stem segment with buds begins to produce clustered buds 7 days after the induction culture in the induction medium, an...
Embodiment 2
[0044] Embodiment 2: a kind of Clematis tissue culture method is characterized in that, comprises the following steps:
[0045] (1) Obtain sterile vaccine materials:
[0046] Cut the newly germinated stems with buds of clematis in spring, wash them under tap water for 60 minutes, soak them in 75% ethanol (volume concentration) for 20 seconds on the ultra-clean workbench, and then use 1% (mass concentration) mercury chloride After soaking in the solution for 15 minutes, rinse with sterile water for 8 times, and dry the surface moisture of the stem section with buds;
[0047] (2) Primary culture:
[0048] Get the stem section with buds after the above-mentioned aseptic treatment, cut into 1cm long sections, each section has at least one well-developed, healthy bud without damage from diseases and insect pests, and inoculate it on the induction medium with a pH value of 5.6, The stem segment with buds begins to produce clustered buds 7 days after the induction culture in the in...
PUM
Abstract
Description
Claims
Application Information
- R&D Engineer
- R&D Manager
- IP Professional
- Industry Leading Data Capabilities
- Powerful AI technology
- Patent DNA Extraction
Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.
© 2023 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap