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Isolation and Extraction of Anti-inflammatory Components of Tongzuan and Test of Anti-inflammatory Activity

A volume fraction, anti-tumor drug technology, applied to medical preparations containing active ingredients, anti-inflammatory agents, plant/algae/fungus/moss components, etc.

Inactive Publication Date: 2017-11-10
GUANGXI NORMAL UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

So far, there is no report on the systematic study of the anti-inflammatory active ingredients of Yaoyao Tongzuan and its related anti-inflammatory products

Method used

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  • Isolation and Extraction of Anti-inflammatory Components of Tongzuan and Test of Anti-inflammatory Activity
  • Isolation and Extraction of Anti-inflammatory Components of Tongzuan and Test of Anti-inflammatory Activity
  • Isolation and Extraction of Anti-inflammatory Components of Tongzuan and Test of Anti-inflammatory Activity

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Embodiment Construction

[0016] refer to figure 1 , figure 2 , the extraction method of anti-inflammation component of copper drill of the present invention is that 20 kg of dried stems of copper drill are cut into small sections, soaked in 95% ethanol with volume fraction for 3 h, heated and refluxed for 3 times, the first time for 3 h , the second time and the third time each 2h, the extract was concentrated under reduced pressure to obtain 1.2 kg of extract. The extract was dissolved in 5% tartaric acid, and filtered to obtain 460 g of acid-water insoluble matter. 460g of the water-insoluble acid was dissolved in water and extracted with ethyl acetate. The ethyl acetate extraction fraction (110 g) was separated by silica gel column chromatography, and gradient eluted with dichloromethane and methanol solvent system (volume ratio of 100:0 to 0:100) to obtain Fr.E1 (3.2 g), Fr. E2 (1.4 g), Fr. E3 (25.0 g), Fr. E4 (40.0 g), Fr. E5 (21.1 g), Fr. E6 (10.5 g) and Fr. E7 (1.3 g) a total of 7 parts. ...

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Abstract

The invention discloses an extraction method for the anti-inflammatory ingredients of erycibe obtusifolia benth. The method comprises the main steps of: 1, soaking in ethyl alcohol, 2, extracting and concentrating to obtain an extract, 3, dissolving the extract in 5% tartaric acid, and filtering to obtain acid water insolubles, 4, extracting the acid water insolubles, and 5, obtaining seven parts of anti-inflammatory ingredients, namely Frs.E1 to E7, wherein through a test, the active ingredients are capable of remarkably inhibiting the release of the NO (nitric oxide) of LPS (lipopolysaccharide)-challenge N9 microglial cells, and free from influence on the survival rate of the microglial cells in a concentration range exerting the inhibition effect. A foundation is laid for preparation for novel medicines.

Description

technical field [0001] The invention relates to the extraction of medicines, in particular to the separation and extraction of anti-inflammatory components of Yao medicine copper diamond and the test of anti-inflammatory activity. Background technique [0002] Copper diamond, a medicinal material commonly used in Guangxi Yao medicine, is a heart vine for tea cornaceae plants Mappianthus iodoides Dried cane of Hand.-Mazz. It is called "dongh nzunx" (copper diamond) in Yao language, also known as yellow diamond, jute bone wind, sweet fruit vine. Function cures mainly expelling wind and dampness, regulating menstruation and promoting blood circulation, relieving pain. For rheumatoid arthritis, rheumatoid arthritis, jaundice, bruises, irregular menstruation, dysmenorrhea, amenorrhea; external use to treat traumatic bleeding, snake bites, one of the "eighteen diamonds" commonly used medicinal materials in Yao medicine. According to literature search, there are only a few domes...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K36/185A61P29/00
Inventor 梁东张贵杰胡峰潘英明
Owner GUANGXI NORMAL UNIV