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Preparation method of vitacoenzyme
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A technology of vitamin enzymes and inoculum volume, which is applied to medical preparations containing active ingredients, pharmaceutical formulas, plant raw materials, etc., can solve the problems of long cycle and low efficiency of solid-state fermentation
Inactive Publication Date: 2014-12-24
中恩(天津)医药科技有限公司
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Problems solved by technology
[0003] The existing production of vizyme mostly uses soybeans or bean dregs as raw materials and adopts solid-state fermentation method for production. The efficiency of solid-state fermentation is low and the cycle is long
Method used
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Embodiment 1
[0020] A preparation method of vizyme, the concrete steps are as follows:
[0021] (1) Bean dregs and water are beaten with water at a weight ratio of 1:10, and then ground through a colloid mill;
[0022] (2) Continue to add glucose, the addition amount is 25g / L, adjust pH to 6.5, pack and sterilize;
[0023] (3) After cooling, inoculate the Pseudocystis arseiii seed liquid according to 10% inoculation amount, and ferment in shake flasks at 28°C and 180r / min;
[0024] (4) Start low-frequency ultrasonic stimulation after 72 hours of fermentation, the ultrasonic frequency is 25kHZ, stimulate once every 6h, each time for 30min, stimulate for 30s, and the interval is 30s;
[0025] (5) Drying, crushing, and the obtained product, after testing, the yield of riboflavin is 0.421mg / L.
Embodiment 2
[0027] A preparation method of vizyme, the concrete steps are as follows:
[0028] (1) Bean dregs and water are beaten with water at a weight ratio of 1:5, and then ground through a colloid mill;
[0029] (2) Continue to add glucose, the addition amount is 10g / L, adjust pH to 7, pack and sterilize;
[0030] (3) After cooling, inoculate the Pseudocystis arseiii seed liquid according to 10% inoculation amount, and ferment in shake flasks at 28°C and 180r / min;
[0031] (4) Start low-frequency ultrasonic stimulation after 72 hours of fermentation, the ultrasonic frequency is 30kHZ, stimulate once every 12h, each time for 30min, stimulate for 30s, and the interval is 30s;
[0032] (5) Drying, crushing, and the obtained product, after testing, the riboflavin yield is 0.410mg / L.
Embodiment 3
[0034] A preparation method of vizyme, the concrete steps are as follows:
[0035] (1) Bean dregs and water are beaten with water at a weight ratio of 1:20, and then ground through a colloid mill;
[0036] (2) Continue to add glucose, the addition amount is 30g / L, adjust pH to 6.5, pack and sterilize;
[0037] (3) After cooling, inoculate the Pseudocystis arseiii seed liquid according to 10% inoculation amount, and ferment in shake flasks at 28°C and 180r / min;
[0038] (4) Start low-frequency ultrasonic stimulation after 72 hours of fermentation, the ultrasonic frequency is 18kHZ, stimulate once every 4 hours, each time for 30 minutes, stimulate for 30s, and the interval is 30s;
[0039] (5) drying, pulverizing, to obtain, after testing, riboflavin yield is 0.486mg / L.
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Abstract
The invention provides a preparation method of vitacoenzyme. The preparation method comprises the following steps: (1) adding bean dregs and water according to the weight ratio of 1:(5-20), pulping and then grinding on a colloid grinder; (2) continuously adding 10-30 g / L of glucose, regulating the pH to be 6.5-7, sub-packaging and sterilizing; (3) inoculating ecemothecium seed liquid according to 10 percent of inoculation quantity after cooling, and shaking a flask and fermenting at the temperature of 28 DEG C at the speed of 180 r / min; (4) fermenting for 72 hours and starting to carry out low-frequency ultrasound stimulation, wherein the ultrasound frequency is 18-30 kHz, the stimulation is performed for 30 minutes once every 4-12 hours, and the time interval is 30 seconds after stimulation for 30 seconds (5) drying and crushing to obtain the vitacoenzyme. The preparation method has the benefits that the yield and the fermentation efficiency of the vitacoenzyme can be obviously improved, the yield of riboflavin as a main component in the vitacoenzyme is improved by about 2 times, and the fermentation period is shortened to 36 hours.
Description
technical field [0001] The invention relates to a preparation method of medicine, in particular to a preparation method of vizyme. Background technique [0002] Vizyme is a drug for treating atrophic gastritis and esophageal epithelial hyperplasia. It is widely used because of its good curative effect and no side effects. Its main components are riboflavin and riboflavin derivatives. Among them, there are 12 kinds of vitamins, B2 and VE contain the most, 18 kinds of amino acids, 23 kinds of trace elements, and others include sugars and crude fibers. [0003] The existing production of vizyme mostly uses soybeans or bean dregs as raw materials and adopts solid-state fermentation method for production. The solid-state fermentation efficiency is low and the cycle is long. Contents of the invention [0004] The technical problem to be solved by the present invention is to provide a preparation method of vizyme. [0005] In order to solve the problems of the technologies des...
Claims
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Application Information
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