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A kind of bacillus cereus wettable powder and its preparation method and application

A technology of Bacillus cereus and wettable powder, which is applied in the field of microorganisms, can solve the problems of high storage cost, potential safety hazards, and easy corruption, and achieve the effects of reducing pollution, reducing storage costs, and low cost

Active Publication Date: 2017-04-26
WUHAN POLYTECHNIC UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, chestnuts are prone to spoilage during storage, and are often stored in low-temperature storage after treatment with chemical antibacterial agents; the storage cost is high, and the use of chemical reagents has potential safety hazards to human health and the environment

Method used

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  • A kind of bacillus cereus wettable powder and its preparation method and application
  • A kind of bacillus cereus wettable powder and its preparation method and application
  • A kind of bacillus cereus wettable powder and its preparation method and application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0022] Screening of Bacillus cereus

[0023] Bacillus cereus CE-D10.3.2 was obtained from the nuts of the storable chestnut variety "Rose Red" in Luotian County, Hubei Province through the steps of isolation, purification and screening.

[0024] The morphological characteristics, physiological and biochemical experiments were used to confirm that the strain was Bacillus cereus, and the 16SrDNA homologous sequence alignment by molecular biological identification method showed that the homology was 100%, and the strain was finally determined to be Bacillus cereus. ).

Embodiment 2

[0026] Preparation of freeze-dried powder of Bacillus cereus

[0027] (1) Bacillus cereus CE-D10.3.2 was inoculated into sterilized LB liquid medium, shaken and cultured at 37°C, 100-120r / min until logarithmic growth phase, to obtain seed liquid, LB liquid medium formula For: tryptone 10g / L, yeast extract 5g / L, sodium chloride 10g / L, pH=7.4~7.6;

[0028] (2) The seed liquid is inoculated in the seed tank according to the inoculum amount of 1~5% (V / V), and the medium in the seed tank is the LB liquid medium of the pass step (1), carried out under the following conditions Cultivation: aeration ratio 1:0.9-1.1, stirring speed 150-200r / min, temperature 35-37°C, tank pressure 0.015-0.020MPa, culture time 46-48h, the content of live bacteria is 1-3×10 9 cfu / mL bacterial suspension;

[0029] (3) After adding 5% glycerin cryoprotectant to the bacterial suspension, place it in a -75~-85℃ ultra-low temperature refrigerator for 2.5-3.5h; , lyophilized for 20-30 hours; control the low ...

Embodiment 3

[0031] Preparation and optimization of Bacillus cereus wettable powder

[0032] 1. Compatibility testing of carriers, wetting agents and dispersants with Bacillus cereus CE-D10.3.2

[0033] The carrier (white carbon black, light calcium carbonate, diatomaceous earth, bentonite, kaolin, corn flour, talcum powder, fineness all passed through a 325 mesh sieve), wetting agent (sodium lauryl sulfate, alkyl naphthalene sulfonate) salt, tea saponin, sodium dodecylbenzenesulfonate), and dispersant (sodium lignosulfonate, polyvinyl alcohol, sodium salt of alkylnaphthalenesulfonic acid condensation polymer, sodium hexametaphosphate, carboxymethyl cellulose Su Na) were added into 100mL LB liquid medium (tryptone 10g / L, yeast extract 5g / L, sodium chloride 10g / L, pH=7.4~7.6) respectively according to the ratio of 5% and mixed evenly, after sterilization Insert 1mL of Bacillus cereus CE-D10.3.2 seed solution, and shake at 37°C for 24h at 100-120r / min. Taking the culture solution without l...

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Abstract

The invention relates to the field of microbes, in particular to a wettable powder of Bacillus cereus and a preparation method and application thereof. A Bacillus cereus wettable powder, comprising the following components: by weight percentage, 50-70% of Bacillus cereus freeze-dried powder, 8-12% of auxiliary agents, 0.5-1.5% of stabilizers, and 0.05% of ultraviolet protective agents ‑0.15%, the balance being the carrier. It is prepared by the following method: uniformly mixing and pulverizing each component to obtain a powder with a mesh number not greater than 150, and keeping the temperature at 0-45°C during the whole preparation process. The Bacillus cereus wettable powder provided by the present invention has a certain effect on common fungal diseases in the storage process of chestnuts and other fruits, such as Chestnut spp. It has good inhibitory effect, is non-toxic to humans and animals, and has low cost; it can well solve the problem of storing chestnuts at room temperature and reduce storage costs.

Description

technical field [0001] The invention relates to the field of microbes, in particular to a wettable powder of Bacillus cereus and a preparation method and application thereof. Background technique [0002] Chestnut, commonly known as "hardcore crops", is one of the important woody food tree species in my country. However, chestnuts are prone to spoilage during storage, and they are often stored in low-temperature storage after treatment with chemical antibacterial agents; the storage cost is high, and the use of chemical reagents has potential safety hazards to human health and the environment. Contents of the invention [0003] The object of the present invention is to provide a Bacillus cereus wettable powder and its preparation method and application, so as to solve the above problems. [0004] The Bacillus cereus provided in the examples of the present invention was preserved in the China Center for Type Culture Collection on April 1, 2014, the preservation number is C...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20A01N63/00A01N25/14A01P3/00C12R1/085
Inventor 程水源李琳玲王燕程华华娟袁红慧张雪花黄小花
Owner WUHAN POLYTECHNIC UNIVERSITY
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