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Method for producing carotenoids by using food organic wastewater

A technology of organic wastewater and carotene, applied in the direction of microorganism-based methods, biochemical equipment and methods, microorganisms, etc., can solve the problem of limited degradation ability, less substrates for carotenoid synthesis, and low yield of carotenoids in bacteria, etc. problem, to achieve the effect of increasing yield, improving utilization rate and improving COD removal rate

Active Publication Date: 2014-12-24
HARBIN INST OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0004] The purpose of the present invention is to solve the problem that PSB has limited degradation ability for organic matter in the existing PSB method for treating food organic wastewater to synthesize carotenoids, and there are fewer substrates for carotenoid synthesis, and the productivity of thallus carotenoids in wastewater treatment low, which hinders the resource recovery process of wastewater treatment, and provides a method for producing carotenoids from food organic wastewater

Method used

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  • Method for producing carotenoids by using food organic wastewater
  • Method for producing carotenoids by using food organic wastewater
  • Method for producing carotenoids by using food organic wastewater

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specific Embodiment approach 1

[0020] Specific embodiment one: this embodiment utilizes food organic waste water to produce carotenoids according to the following steps:

[0021] 1. Adjust the pH value of food organic wastewater to 6.5-8.0, and then add Rhodobacter sphaericus and growth-promoting bacteria in the logarithmic growth phase;

[0022] 2. The food organic wastewater added with Rhodobacter sphaericus and growth-promoting bacteria is treated for 72-96 hours under micro-aerobic conditions with a light intensity of 500-3000 lux and a treatment temperature of 25-30°C;

[0023] 3. Recovery and extraction to obtain carotenoids;

[0024] Wherein, in step 1, 360 mg of Rhodobacter sphaeroides bacterium liquid was added to every liter of food organic wastewater, and the concentration of Rhodobacter spheroides bacterium liquid was 6.2×10 8 ~7.0×10 8 cfu / mL;

[0025] In step 1, 20-80 μL of growth-promoting bacteria solution was added to every 100 ml of food organic wastewater. The growth-promoting bacteria...

specific Embodiment approach 2

[0030] Embodiment 2: The difference between this embodiment and Embodiment 1 is: the COD value of food organic wastewater is 8000-15000 mg / L, the TN value is 500-2000 mg / L, and the TP value is 20-50 mg / L. Other steps and parameters are the same as those in Embodiment 1.

specific Embodiment approach 3

[0031] Embodiment 3: The difference between this embodiment and Embodiment 1 or 2 is that in Step 1, 30-70 μL of growth-promoting bacteria solution is added to every 100 ml of food organic wastewater. Other steps and parameters are the same as those in Embodiment 1 or 2.

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Abstract

The invention discloses a method for producing carotenoids by using food organic wastewater, relating to a method for producing carotenoids by using organic wastewater. By adopting the method, the problems that in an existing method for synthesizing carotenoids by using PSB (photosynthetic bacteria) to treat food organic wastewater, the capacity of PSB for degrading organic matters is limited, relatively less substrates for synthesizing carotenoids are provided, the yield of bacterial carotenoids in wastewater treatment is low, and the recycling process of wastewater treatment is blocked can be solved. The method comprises the following steps: firstly, adjusting the pH value of the food organic wastewater, and then adding rhodobacter sphaeroides and growth-promoting bacteria; secondly, performing treatment for 72-96h under micro-aerobic conditions; and thirdly, recycling and extracting to obtain carotenoids. Compared with an existing conventional technique for treating the food organic wastewater by independently using PSB, the bacterium yield and carotenoid yield can be increased by more than 25% and 100% respectively by adopting the method disclosed by the invention, and the removal rate of COD in the food organic wastewater also can be increased by more than 78%.

Description

technical field [0001] The invention relates to a method for producing carotenoids by utilizing organic waste water. Background technique [0002] Food organic wastewater is a type of wastewater with a large discharge volume, because it is non-toxic and rich in macromolecular nutrients such as sugar, protein and cellulose. Food organic wastewater is a kind of wastewater rich in carbon and nitrogen sources. It has high hydraulic load and organic load, and it pollutes the environment very strongly, especially causing eutrophication of water bodies and destroying the self-purification ability of water bodies. At present, biological methods are mainly used to treat food organic wastewater, including activated sludge method and biofilm method; however, the process flow of activated sludge method and biofilm method is complicated, and a large amount of surplus sludge will be generated, the disposal cost is high, and the degree of resource utilization is high. low, it is also easy...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P39/00C12P23/00C12R1/085C12R1/01
Inventor 刘淑丽张光明李相昆张杰
Owner HARBIN INST OF TECH
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