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A technology of hybrid breeding and croissant melon, which is applied in the fields of botany equipment and methods, seed and rhizome treatment, plant gene improvement, etc., and can solve the problems of hybridization of crocodile melon artificially planted, etc.
Inactive Publication Date: 2015-01-07
黄惠民
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[0008] The hybrid breeding and cultivation of oxhorn melon (including white-flowered oxhorn melon and large oxhorn melon) is currently in a ...
Method used
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Embodiment 1
[0042] In Dongchuan District, Kunming City, Yunnan Province, the crossbreeding work was carried out, and the female parent (hermaphrodite variety) growing in Yuanyang, Honghe, Yunnan and the male parent of the growing Longhorn melon seed in Dongchuan District, Kunming City were collected for cross breeding, and the numbers were recorded first. Combination, single plant, system, phylogenetic group, etc. serial numbers and character records.
[0043] In the process of hybridization, trait complementation, trait inheritance, geographical origin varieties, different effects of reciprocal crossing, pollen collection, female parent preparation, hybridization, bagging after pollination, seed collection, hybrid seed stratification, sowing, seedling cultivation, observation Screen the F1 homozygous varieties of the first generation of hybrids (offspring generation). The fiber length of the offspring is generally 31-33mm.
Embodiment 2
[0045] In Wuzhou City, Guangxi, the crossbreeding work was carried out, and the female parent of Panzhihua, Sichuan and the first generation of hybrids grown in Dongchuan District, Kunming, Kunming City were collected as the male parent, and the hybrid combination, single plant, system, System group numbers and trait records.
[0046] In the process of hybridization, trait complementation, trait inheritance, geographical origin varieties, different effects of reciprocal crossing, pollen collection, female parent preparation, hybridization, bagging after pollination, seed collection, hybrid seed stratification, sowing, seedling cultivation, observation Screen the second-generation F2 homozygous varieties of hybrids. The fiber length of its hybrid second-generation F2 reaches 33-34mm.
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Abstract
The invention relates to a method for crossbreeding Calotropis R. Br. The method comprises the following steps: (1) selecting a female parent, wherein the length of a fiber main body is over 30mm, the outer diameter of fruits is not smaller than 8cm, and the number of fruits on a single plant is not less than 10; (2) selecting a male parent, wherein the length of a fiber main body is not shorter than 29mm, the outer diameter of fruits is more than 10cm, and the number of fruits on a single plant is not less than 10; (3) collecting pollen; (4) hybridizing; (5) stratifying hybridized seeds; (6) sowing; (7) screening to obtain a hybrid generation F1, and analyzing an exogenous gene homozygous plant by using amplification results, and verifying in F2. The process for selecting advantageous female and male parent materials is carried out multiple times to obtain hybridized or backcrossed hybrid seedling homozygous plants with excellent properties generation to generation from F1-F8 for planting. The method fills in the blank in crossbreeding cultivation techniques of Calotropis R. Br. The first purpose of the method is to prepare Calotropis R. Br seed fibers (the length of Calotropis R. Br fibers is more than 30mm) serving as a novel Calotropis R. Br fiber textile raw material and the second purpose of the method is to obtain stem leaves of Calotropis R. Br as novel Calotropis R. Br biomedicine and biological energy raw materials.
Description
technical field [0001] The invention relates to a method for hybrid breeding of plants, in particular to a method for hybrid breeding of horned melon. Background technique [0002] 1. Chinese name: horned melon; Latin name: ( Apocynaceae; )nickname:( Calotropis R. Br ). [0003] 2. Chinese name: white flower horn melon. [0004] 3. Chinese name: big horned melon; [0005] Erect shrub, perennial up to 3m-4m, flowering and fruiting for about 11 months throughout the year. [0006] The oxhorn melon, the white-flowered oxhorn melon, and the big oxhorn melon are wild plants, and the large-scale cultivation of oxhorn melon in the agriculture and forestry planting industry is blank. In China, horned melon lives wild in a small amount of barren mountains and wastelands in arid and semi-arid arid and hot valleys in Yunnan, Sichuan, Qiong, Guangxi, Guangdong and other provinces, or grows in a small amount in the Jinsha River Basin or the Red River Basin, or grows in a small amou...
Claims
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Application Information
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