Bacillus subtilis B51 and application of bacillus subtilis B51
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A technology of Bacillus subtilis and bacterial agents, applied in the field of Bacillus subtilis B51 and its application in the prevention and treatment of Dendrobium candidum root rot, can solve serious problems, economic losses, and death in pieces
Active Publication Date: 2015-01-07
JIANGSU POLYTECHNIC COLLEGE OF AGRI & FORESTRY
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[0008] However, for more than ten years, with the continuous expansion of the artificial cultivation area of Dendrobium candidum, the occurrence of diseases and insect pests is common and serious, especially the occurrence o
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Embodiment 1
[0039]Embodiment 1: the preparation method of Bacillus subtilis (Bacillus subtilis) B51 inoculum: inoculate Bacillus subtilis (B.subtilis) B51 in the fermentation medium, pH6.5, liquid volume in 500mL conical flask is 300mL, 28 °C-35 °C, shaker speed 120 rpm, 48 hours, the obtained fermentation liquid is the original bacterial liquid for preventing and treating Dendrobium officinale root rot.
[0040] Wherein the formula of fermentation medium is: 1000mL distilled water, beef extract 2g, peptone 5g, sodium chloride 5g, pH6.5.
Embodiment 2
[0041] Example 2: The preparation method of Bacillus subtilis (B.subtilis) B51 inoculum: Inoculate Bacillus subtilis B51 in the fermentation medium, pH 7.5, liquid volume in 500mL Erlenmeyer flask is 300mL, 28°C-35°C , shaking table speed 160 rev / min, 72 hours, the fermented liquid that obtains is the original bacterial liquid of preventing and treating Dendrobium officinale root rot.
[0042] Wherein the formula of fermentation medium is: 1000mL distilled water, beef extract 5g, peptone 10g, sodium chloride 5g, pH7.5.
Embodiment 3
[0043] Example 3: The preparation method of Bacillus subtilis (B.subtilis) B51 inoculum: Inoculate Bacillus subtilis B51 in the fermentation medium, pH 7, liquid volume in 500mL Erlenmeyer flask is 300mL, 28°C-35°C, Shaking table rotating speed 150 revs / min, 55 hours, the fermented liquid that obtains is the original bacterium liquid of preventing and treating Dendrobium officinale root rot.
[0044] Wherein the formula of fermentation medium is: 1000mL distilled water, beef extract 3g, peptone 8g, sodium chloride 5g, pH 7.
[0045] The application of Bacillus subtilis B51 of the present invention will be described in detail below in conjunction with experimental examples.
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Abstract
The invention discloses bacillus subtilis B51. The preservation serial number of the bacillus subtilis B51 is CGMCCNo.7541; the preservation date of the bacillus subtilis B51 is April 28, 2013; the bacillus subtilis B51 is named as Bacillus subtilis and has been preserved in the general microbiology culture preservation management center of China. The bacillus subtilis B51 plays a good role in inhibitingvarious pathogenic funguses such as the dendrobium officinale ceitocybe bescens, the wheat scab and the grey mould fruit rot of strawberries, and antagonistic bacteria play a good role in preventing and treating other plant diseases; the bacillus subtilis B51 has the advantages of being high in breeding speed, easy to operate, convenient to produce and apply, high in resilience capacity, capable of being artificially cultivated and the like; in particular, the bacillus subtilis B51 is of important significance in preventing and treating the dendrobium officinale ceitocybe bescens caused by the funguses; culture conditions are simple, preservation is easily carried out, industrial production is easily achieved, and the bacillus subtilis B51 has good development and application prospects.
Description
technical field [0001] The invention belongs to the field of plant protection, in particular to a Bacillus subtilis B51 and its application in preventing and treating root rot of dendrobium officinale. Background technique [0002] In recent years, with the general concern of the public on the safety of chemical pesticides in food and the environment, and the prohibition of some dangerous chemical pesticides, people are seeking new ways to control plant diseases safely and effectively, and the use of beneficial microorganisms to control plant diseases has attracted increasing attention. It introduces exogenous antagonistic bacteria beneficial to plants into the broken plant natural ecosystem, or promotes the growth of natural beneficial microbial populations by regulating the environment, and exhibits biological control activity, and achieves disease prevention through the interaction between microorganisms Purpose. At present, the biocontrol of plant diseases has become th...
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IPC IPC(8): C12N1/20A01N63/00A01P3/00C12R1/125
CPCA01N63/00C12N1/205C12R2001/125
Inventor 赵桂华席刚俊赵楠
Owner JIANGSU POLYTECHNIC COLLEGE OF AGRI & FORESTRY
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