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Quality control marker and its use in herbs authentication

A technology of markers and Chinese herbal medicines, applied in the field of quality control markers, can solve problems that are not suitable for fast, efficient, low-cost applications, complex methods, and difficult to promote to commercial scale applications.

Active Publication Date: 2015-01-14
HONG KONG BAPTIST UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, this method is complicated and cumbersome, and is not suitable for fast, efficient and low-cost applications
Therefore, the invention disclosed above is difficult to be promoted to the application of commercial scale

Method used

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  • Quality control marker and its use in herbs authentication
  • Quality control marker and its use in herbs authentication
  • Quality control marker and its use in herbs authentication

Examples

Experimental program
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Effect test

Embodiment

[0041] Quantitative analysis of HPGPC

[0042] Acetonitrile and ammonium acetate for high performance liquid chromatography (HPLC) analysis were purchased from Merck (Darmstadt, Germany). Deionized water was prepared with a Millipore Milli Q-Plus system (Millipore, Bedford, MA, USA). Trifluoroacetic acid (TFA) used for acid hydrolysis of carbohydrates was obtained from Riedel-de Haön (Honeywell International Inc., Germany). 1-Phenyl-3-methyl-5-pyrazolone (PMP) for monosaccharide derivatization was purchased from Sigma (St. Louis, MO, USA). Reference substances D-galacturonic acid monohydrate (GalA), D-glucuronic acid (GlcA), L-arabinose (Ara), D-mannose (Man), D-galactose (Gal), D -Glucose (Glc), L-rhamnose monohydrate (Rha), D-fucose (Fuc), D-ribose (Rib) and a series of glucans with different molecular weights (1kDa, 5kDa, 12kDa, 25kDa, 50kDa, 80kDa, 150kDa, 270kDa, 410kDa and 670kDa) were purchased from Sigma (St. Louis, MO, USA).

[0043] Water Extraction: at 100 o C,...

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Abstract

The present invention relates to a quality control marker and method of using such marker in qualitative and quantitative authentication of Dendrobium officinale Kimura et Migo, which is known as a Chinese medicine under the name of Tiepi Shihu ().

Description

[0001] This application claims the benefit of priority to US Patent Application No. 13 / 940944, filed July 12, 2013, the contents of which are incorporated herein by reference. field of invention [0002] The present invention relates to quality control markers, and methods for qualitatively and quantitatively identifying Chinese herbal medicinal materials (especially suitable for, but not limited to, Dendrobium species) using such markers. More specifically, the present invention relates to chemical markers, and their rapid, efficient, Low-cost identification of Dendrobium officinale ( Dendrobium officinale Kimura et Migo ), the application in the well-known and precious traditional Chinese medicine Dendrobium candidum. technical background [0003] Due to the complex structure and large molecular weight of carbohydrates (especially polysaccharides and oligosaccharides) contained in Chinese herbal medicines, the quality control of these carbohydrates is still full of challen...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N30/02
CPCY10T436/143333G01N33/0098Y10T436/25375
Inventor 韩全斌陈虎彪许军
Owner HONG KONG BAPTIST UNIV
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