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Method for extracting oil from oil production microbes

A technology for oil-producing microorganisms and oil extraction, applied in the direction of fat production, fat oil/fat production, etc., can solve the problems of high energy consumption and low oil extraction rate, and achieve low energy consumption, high oil extraction rate, and good separation effects

Inactive Publication Date: 2015-01-21
CHINA PETROLEUM & CHEM CORP +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] The purpose of the present invention is to overcome the defects of low oil extraction rate and high energy consumption in the prior art of extracting oil from oleaginous microorganisms, and provide an efficient and economical method for extracting oil from oleaginous microorganisms

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0042] This example is used to illustrate the method for extracting oil from oleaginous microorganisms of the present invention.

[0043] (1) Weigh 45 g of 11% by weight water slurry containing Schizochytrium sp., and adjust the pH value of the water slurry containing Schizochytrium sp. to 8 with NaOH;

[0044] (2) Put the water slurry containing Schizochytrium algae in a mechanically stirred reactor, and under the hydrothermal condition at a temperature of 130°C, the stirring power is 0.6kW per cubic meter of the material to be stirred (using Shandong Provincial Juan Chengyongxing Instrument Factory YXMD analog booster electric stirrer for stirring, the same below), and stirring for 20 minutes to obtain the schizophyte algal dissolving slurry;

[0045] (3) Use ferric chloride to adjust the schizopotent algal dissolving slurry to a pH value of 7.0, at 90°C, with a stirring power of 0.6kW per cubic meter of the material being stirred, stir for 20 minutes, and then stand for str...

Embodiment 2

[0047] This example is used to illustrate the method for extracting oil from oleaginous microorganisms of the present invention.

[0048] (1) Weigh 35 g of 14% by weight water slurry containing Schizochytrium sp., and adjust the pH value of the water slurry containing Schizochytrium sp. to 10 with NaOH;

[0049] (2) Put the water slurry containing Schizochytrium algae in a mechanically stirred reactor, under hydrothermal conditions at a temperature of 120°C, with a stirring power of 0.2kW per cubic meter of material to be stirred, and stir for 30 minutes, Obtain schizochytrium algae lysate;

[0050] (3) Use aluminum sulfate to adjust the schizopotent algal dissolving slurry to a pH value of 6.8. At 80°C, with a stirring power of 0.2kW per cubic meter of the material being stirred, stir for 30 minutes, then stand to separate and collect The upper oil phase obtained 2.26g of oil, and the calculated oil extraction rate was 46.1%.

Embodiment 3

[0052] This example is used to illustrate the method for extracting oil from oleaginous microorganisms of the present invention.

[0053] (1) Weigh 55 g of 9% by weight water slurry containing Schizochytrium sp., and adjust the pH value of the water slurry containing Schizochytrium sp. to 12 with NaOH;

[0054] (2) Put the water slurry containing Schizochytrium algae in a mechanically stirred reactor, under hydrothermal conditions at a temperature of 150°C, with a stirring power of 0.3kW per cubic meter of material to be stirred, and stir for 15 minutes, Obtain the lysate of Schizochytrium algae;

[0055] (3) Use ammonium chloride to adjust the schizopotter algal dissolving slurry to a pH value of 7.2. At 70°C, with a stirring power of 0.3kW per cubic meter of the material being stirred, stir for 10 minutes, and then stand for stratification. The upper oil phase was collected to obtain 1.95 g of oil, and the calculated oil extraction rate was 39.4%.

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Abstract

The invention discloses a method for extracting oil from oil production microbes. The method is characterized in that the method comprises the following steps: 1, adjusting the pH value of a water slurry containing the oil production microbes to 7.5-14; 2, stirring under hydrothermal conditions to dissolve out oil in the cells of the oil production microbes in order to obtain an oil production microbes dissolved slurry containing oil; and 3, adjusting the pH value of the oil production microbes dissolved slurry to 6.8-7.2 by using an acidic flocculating agent under stirring, allowing the obtained slurry to stand for layering, and separating the obtained upper layer oil, wherein the acidic flocculating agent is a flocculating agent which is acidic after dissolved in water. The method for extracting oil from oil production microbes allows the pH value of the oil production microbes dissolved slurry to 6.8-7.2 by using the acidic flocculating agent to successfully realize the demulsification of the oil production microbes dissolved slurry in order to thoroughly flocculate microbe residues, so an oil phase and a water phase can be well separate, the oil extraction rate is high, and the energy consumption is low.

Description

technical field [0001] The invention relates to the technical field of oleaginous microorganism engineering, in particular to a method for extracting oil from oleaginous microorganisms. Background technique [0002] Due to the rapid proliferation of microbial cells, short production cycle, wide sources of raw materials required for microbial growth, low price, and microbial growth is not limited by seasons and climate changes, continuous large-scale production is possible, production costs are low, and the oil content of oil-producing microorganisms is high. , therefore, the use of oleaginous microorganisms to produce oil has become an important means to alleviate the global energy crisis. [0003] Schizochytrium and microalgae can be used as important sources of lipid raw materials for biological liquid fuels due to their high intracellular lipid content. Especially with the increasing shortage of world energy sources such as oil, the production of biofuels from animal and...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C11B1/00C11B1/14C11B1/10
CPCC11B1/00C11B1/14
Inventor 于鹏荣峻峰林伟国周旭华
Owner CHINA PETROLEUM & CHEM CORP
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