Industrial production method of cephalotaxus alkaloid

A production method and alkaloid technology, which are applied in the field of plant endophyte separation and microbial fermentation engineering, can solve the problems of low yield of Torreya alkaloids, difficulty in meeting living needs, slow growth of tree species, etc., and shorten the time required for screening. , The screening results are efficient and the metabolism is vigorous.

Inactive Publication Date: 2015-01-21
LUOYANG INST OF SCI & TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

At present, crude torreya resources are scarce, and the tree species grows slowly, and the yield of crude torreya alkaloids is low, which is difficult to meet people's living needs. At the same time, it also causes the pri

Method used

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  • Industrial production method of cephalotaxus alkaloid
  • Industrial production method of cephalotaxus alkaloid

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Effect test

Embodiment 1

[0023] A kind of industrial production method of crude torreya alkaloid, comprises the following steps:

[0024] Step 1. Selection and processing of explants

[0025] Choose the root, stem and leaf of perennial thick torreya as explant, clean the explant with running water, then cut the root into 5-6cm long pieces, remove the old bark and branches and leaves on the stem, and set aside;

[0026] Step 2, sterilization of explants

[0027] Put the treated explants into the ultra-clean workbench, first sterilize them with alcohol with a volume concentration of 75% for 5 minutes, then sterilize them with mercuric chloride with a mass concentration of 0.1% for 12 minutes, wash them with sterile water for 4 times, and put The processed explants were put into sterile water and set aside;

[0028] Step 3, purification and isolation of crude Torreya strain

[0029] Insert the sterilized explants into the culture bottles equipped with PDA medium respectively, and place the culture bot...

Embodiment 2

[0055] A kind of industrial production method of crude torreya alkaloid, comprises the following steps:

[0056] Step 1. Selection and processing of explants

[0057] Choose the root, stem and leaf of perennial thick torreya as explant, clean the explant with running water, then cut the root into 5-6cm long pieces, remove the old bark and branches and leaves on the stem, and set aside;

[0058] Step 2, sterilization of explants

[0059] Put the treated explants into the ultra-clean workbench, first sterilize them with alcohol with a volume concentration of 75% for 5 minutes, then sterilize them with mercuric chloride with a mass concentration of 0.1% for 12 minutes, wash them with sterile water for 5 times, and put The processed explants were put into sterile water and set aside;

[0060] Step 3, purification and isolation of crude Torreya strain

[0061] Insert the sterilized explants into the culture bottles equipped with PDA medium respectively, and place the culture bot...

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Abstract

The invention belongs to the field of plant endophyte separation and microorganism fermentation engineering, and in particular relates to an industrial production method of cephalotaxus alkaloid. The industrial production method comprises the following steps: by adopting roots, stems and leaves of perennial cephalotaxus as explants, cutting into strips, sterilizing, then culturing in a culture medium to grow cephalotaxus symbiotic bacteria, then separating the cephalotaxus symbiotic bacteria according to different strains and respectively performing culture and fermentation, concentrating a fermentation liquid and screening to obtain the strains capable of metabolizing cephalotaxus alkaloid, performing fermentation and culture, separating the fermentation liquid from a culture product, and performing alcohol degradation, suction filtration and drying to prepare cephalotaxus alkaloid. According to the industrial production method disclosed by the invention, activated strains are firstly inoculated into a shake flask filled with a fermentation culture medium for culturing, and then are subjected to amplification culture by virtue of a four-stage seeding tank to ensure that the strains are stable in physiological state and strong in activity, and can rapidly grow after transplanting; and a great number of high-quality strains can be obtained in a short time, the fermentation time can be shortened, and the production efficiency can be improved.

Description

technical field [0001] The invention belongs to the field of plant endophytic bacteria isolation and microbial fermentation engineering, and in particular relates to an industrial production method of crude torreya alkaloids. Background technique [0002] Torreya is a unique plant in my country, which has high nutritional value and economic value. Torreya alkaloid is one of the important active ingredients of Torreya, which has anti-cancer effects and is often used in medicine. At present, crude torreya resources are scarce, and the tree species grows slowly, and the yield of crude torreya alkaloids is low, which is difficult to meet people's living needs. At the same time, it also causes the price of crude torreya alkaloid drugs to be expensive. Therefore, how to quickly increase the crude torreya alkaloids through industrial production The production of alkaloids has become one of the important issues for people. Contents of the invention [0003] The object of the pres...

Claims

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Application Information

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IPC IPC(8): C12P17/18
Inventor 王祖华王安亭李永程陈华军黄向东王佳伟
Owner LUOYANG INST OF SCI & TECH
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