Tissue culture and rapid propagation method of plantain lily

A technology of tissue culture and hosta, which is applied in the field of tissue culture and rapid propagation of Phnom Penh hosta, can solve the problems of slow propagation speed of ramets, large variation of seed reproduction of Phnom Penh hosta, and inability to meet the needs of industrialization, etc. The effect of seedling reproduction coefficient

Inactive Publication Date: 2015-01-28
HEILONGJIANG ACAD OF SCI INST OF NATURAL RESOURCES
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Problems solved by technology

[0003] The present invention aims at optimizing the regeneration system and takes maintaining genetic stability as the core to provide a method for establishing a high-efficiency regeneration system of Phno

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  • Tissue culture and rapid propagation method of plantain lily
  • Tissue culture and rapid propagation method of plantain lily
  • Tissue culture and rapid propagation method of plantain lily

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[0017] Conditions for the implementation of the present invention: sterile inoculation room, sterile culture room, transitional culture room, greenhouse.

[0018] The essence of the present invention is that the new plant maintains the genetic characteristics of a healthy female parent, the regenerated plant has good quality and a large reproduction coefficient.

[0019] A method for establishing a plant regeneration system of Phnom Penh hosta plant. Adventitious buds are differentiated from the side buds of the plant as explants, and good small plants are cultivated through the methods of proliferation, strong seedlings, rooting, transplanting and domestication, and the specific steps are as follows:

[0020] 1) Selection of explants: In spring, select the buds from healthy plants and cut them off with a sharp blade;

[0021] 2) Explant sterilization: cut off 2 / 3 of the leaves, first rinse with tap water, rinse with appropriate amount of detergent and tap water for 30-40 minutes, blot...

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Abstract

The invention relates to a tissue culture and rapid propagation method of plantain lily. The production problems that the division propagation speed of the variety is low and the propagation coefficient is very low are solved. The method comprises the following steps: 1, explant selection; 2, explant sterilization; 3, explant primary culture; 4, proliferation culture; 5, strong seedling culture; 6, rooting culture; 7, transplanting domestication. Plant base differentiated buds are used as explants, an MS is used as a basic culture medium, and a method of a plantain lily regeneration system is established through the regulation of NAA (Naphthalene Acetic Acid), 6-benzylaminopurine (6BA) and indolybutyric acid (IBA). One proliferation subculture period is four to six weeks, the proliferation coefficient is controlled at four to six times and is not affected by seasons, the seedling reproduction coefficient can be greatly improved, a scientific and efficient propagation method is provided for the development and utilization of the variety, and industrial production is carried out to meet production requirements.

Description

technical field [0001] The invention relates to a method for tissue culture and rapid propagation of Phnom Penh hosta. technical background [0002] Phnom Penh hosta is a perennial herbaceous perennial flower of Liliaceae, Hosta genus, with white or purple flowers, cold-resistant and shade-tolerant, and grows luxuriantly in shaded and ventilated places. Phnom Penh Hosta, which is introduced from the United States, is a new variety of landscaping in recent years. It is loved by people. Its leaves are thick and inlaid with yellow edges. The white flowers are fragrant. It is not only an excellent garden landscape flower, but also a very Development prospects of medicinal plants. Because the traits of the offspring of Phnom Penh Hosta seeds are prone to change, and it takes three years to bloom; this variety grows slowly, the propagation speed of ramets is slow, and the multiplication coefficient is extremely low. It can only multiply 2-4 times per year, which greatly affects P...

Claims

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Application Information

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IPC IPC(8): A01H4/00
Inventor 曲彦婷唐焕伟张冬青张兴李黎陈菲韩辉刘淑艳张鹏远熊燕梁鸣
Owner HEILONGJIANG ACAD OF SCI INST OF NATURAL RESOURCES
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