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Anti-fatigue purposes and extraction method of antioxidant active ingredients of Chinese red pine needles

A technology of antioxidant activity and pine needles of masson pine, applied in the direction of medical preparations containing active ingredients, functions of food ingredients, food ingredients, etc.

Active Publication Date: 2015-01-28
GUANGZHOU MEDICAL UNIV
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Problems solved by technology

[0003] At present, the research on the antioxidant activity of pine needles of masson pine mainly focuses on the extraction, separation and functional evaluation of components that may have some antioxidant activity. There is no report on the research of screening out active ingredients with anti-oxidation

Method used

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  • Anti-fatigue purposes and extraction method of antioxidant active ingredients of Chinese red pine needles
  • Anti-fatigue purposes and extraction method of antioxidant active ingredients of Chinese red pine needles
  • Anti-fatigue purposes and extraction method of antioxidant active ingredients of Chinese red pine needles

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Embodiment Construction

[0031] 1. Verification of the active components of the antioxidant activity of Pinus massoniana pine needles extracted by water extraction method:

[0032] The invention uses a water extraction method to extract the anti-oxidation active ingredients of the pinus massoniana pine needles. In the single factor test, in addition to investigating the effect of solid-liquid ratio, time and temperature on the extraction effect, and based on the single factor results, three levels with better factors were selected for orthogonal test to optimize the optimal extraction process conditions.

[0033] Determination of the total antioxidant capacity of Pine massoniana pine needle extract: The body's defense system has a close relationship with its antioxidant capacity and health. The defense system has two systems, enzymatic and non-enzymatic. Many enzymes are based on trace elements Active centers, such as: superoxide dismutase (SOD), glutathione peroxidase (GSH-PX), catalase (CAT), glutat...

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Abstract

The invention discloses anti-fatigue purposes of antioxidant active ingredients of Chinese red pine needles and a method for extracting the antioxidant active ingredients of Chinese red pine needles. The method for extracting the antioxidant active ingredients of Chinese red pine needles comprises the following steps: step 1, removing dead leaves and leaf stalks of fresh pine needles, flushing under running water, and airing in shade; step 2, shearing into small sections of 1cm by using ceramic scissors, grinding for 30 seconds by using an ultrafine grinder to obtain pine needle residues, and shading for later use; step 3, taking 10g of pine needle residues, and adding ultrapure water according to a material water ratio of 1 to (10-25) (g to ml); step 4, adding the mixture into a round-bottom flask, arranging the flask in Soxhlet extraction equipment, and leaching at the temperature of 70-100 DEG C for 60-150 minutes; and step 5, performing vacuum filtration on the leaching solution, thereby obtaining the filtrate containing the antioxidant active ingredients. The method has the beneficial effects that a novel natural antioxidant product is provided.

Description

technical field [0001] The invention belongs to the technical field of antioxidant compounds, and relates to an anti-fatigue application and an extraction method of an antioxidant active component of pine needles of masson pine. Background technique [0002] Pinus massoniana pine needles are needle-shaped leaves of Pinaceae plants. In recent years, studies have found that they are rich in natural antioxidant active substances such as flavonoids, lignans, vitamins, polysaccharides and trace elements, and are non-toxic and have no side effects. They can be used as Good source of natural antioxidants and antioxidant functional foods. [0003] At present, the research on the antioxidant activity of pine needles of masson pine mainly focuses on the extraction, separation and functional evaluation of components that may have some antioxidant activity. There is no report on the research of screening out active ingredients with antioxidant activity. Therefore, this study intends t...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K36/15A23L2/38A61P39/00
CPCA23L2/38A61K36/15A23V2002/00A61K2236/51A61K2236/39A61K2236/331A61K2236/333A61K2236/35A61K2236/15A23V2250/21A23V2200/302
Inventor 雷毅雄陈骁熠黄佳銮杨丽华林楚慧张晋蔚王响
Owner GUANGZHOU MEDICAL UNIV
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