Ultra-low temperature preservation method of Chinese red pine pollen

A technology of cryopreservation and masson pine, which is applied in the fields of plant preservation, botanical equipment and methods, plant gene improvement, etc. It can solve the problems of inconvenient work in germplasm research and hybrid breeding, achieve good pollen survival rate, and reduce heterogeneous ice crystals The formation of pollen, the effect of improving the survival rate of pollen

Pending Publication Date: 2018-11-02
GUANGXI FORESTRY RES INST
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Studies have shown that in the natural state, the lifespan of masson pine pollen is only one month, which brings great inconvenience to germplasm research and cross-breeding work, so medium and long-term preservation of pollen is particularly important

Method used

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  • Ultra-low temperature preservation method of Chinese red pine pollen
  • Ultra-low temperature preservation method of Chinese red pine pollen
  • Ultra-low temperature preservation method of Chinese red pine pollen

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0030] A method for cryopreservation of masson pine pollen, comprising the steps of:

[0031] (1) Collection of flower spikes: From March 1-5, 2018, pollen of Pinus massoniana in Nanning, Guangxi was collected. When the microspore leaf balls of the male cones of Pinus massoniana turned slightly yellow to loose powder, the flower spikes were collected in the morning on a sunny day. , use scissors to cut the spikes, wrap them in kraft paper, and bring them back to the laboratory;

[0032] (2) Pollen collection: Put the flower spike sticks in a dried 250ml Erlenmeyer flask, place them in a constant temperature shaking incubator, and shake at a constant speed of 120rpm for 48h, during which time keep a constant temperature of 27°C and a light of 2000lux; after the pollen disperses , tilt the Erlenmeyer flask 45° downward, pat the bottom of the Erlenmeyer flask with the palm of your hand, collect the pollen on the sulfuric acid paper, and collect once every 12 hours;

[0033] (3) ...

Embodiment 2

[0044] A method for cryopreservation of masson pine pollen, comprising the steps of:

[0045] (1) Collection of flower spikes: From March 1 to 5, 2018, the pollen of Pinus massoniana in Nanning, Guangxi was collected. When the microspore leaf balls of the male cones of Masson pine were slightly yellowed to loose powder, the flower spikes were collected in the morning on a sunny day. , use scissors to cut the spikes, wrap them in kraft paper, and bring them back to the laboratory;

[0046] (2) Pollen collection: Put the flower spike sticks into a dried 250ml Erlenmeyer flask, place them in a constant temperature shaking incubator, and shake at a constant speed of 120rpm for 36h, during which time keep a constant temperature of 27°C and a light of 2000lux; after the pollen disperses , tilt the Erlenmeyer flask 60° downward, pat the bottom of the Erlenmeyer flask with the palm of your hand, collect the pollen on the sulfuric acid paper, and collect once every 12 hours;

[0047] ...

Embodiment 3

[0058] A method for cryopreservation of masson pine pollen, comprising the steps of:

[0059] (1) Collection of flower spikes: From March 1 to 5, 2018, the pollen of Pinus massoniana in Nanning, Guangxi was collected. When the microspore leaf balls of the male cones of Masson pine were slightly yellowed to loose powder, the flower spikes were collected in the morning on a sunny day. , use scissors to cut the spikes, wrap them in kraft paper, and bring them back to the laboratory;

[0060] (2) Pollen collection: Put the flower spike sticks into a dried 250ml Erlenmeyer flask, place them in a constant temperature shaking incubator, and shake at a constant speed of 120rpm for 36h, during which time keep a constant temperature of 27°C and a light of 2000lux; after the pollen disperses , tilt the Erlenmeyer flask 60° downward, pat the bottom of the Erlenmeyer flask with the palm of your hand, collect the pollen on the sulfuric acid paper, and collect once every 12 hours;

[0061] ...

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Abstract

The invention discloses an ultra-low temperature preservation method of Chinese red pine pollen. The method comprises the steps of flower spike collection, pollen collection, pollen drying, pollen freezing preservation, pollen unfreezing, in vitro pollen germination and the like. The ultra-low temperature pollen preservation method is optimized aiming at the characteristics of the Chinese red pinepollen, and the method is simple and convenient, is simple in operation, prolongs the preservation time and the preservation life of the Chinese red pine pollen, improves the utilization ratio of theChinese red pine pollen and solves the problems of hybrid seed production such as blooming of flowers at different times of the Chinese red pine.

Description

technical field [0001] The invention relates to a method for ultra-low temperature preservation of forest germplasm resources, in particular to a method for ultra-low temperature preservation of masson pine pollen. Background technique [0002] Pinus massoniana Lamb (Pinus massoniana Lamb) is an evergreen tall tree of the Pinaceae genus. It is the main native tree species of my country's specialty, and it is also the most widely distributed and most resourceful forest community in the southern subtropical region. It has fast growth, excellent material, strong adaptability, The characteristics of high comprehensive utilization rate occupy an extremely important position in the construction of forest resources and ecological development. Due to the large distribution of Pinus massoniana and its rich genetic variation, the preservation of its germplasm resources is of great significance. [0003] Pollen is one of the forms of plant germplasm, which contains all gene types of th...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01N3/00A01H1/02A01D91/04
CPCA01D91/04A01H1/02A01N3/00
Inventor 张晓宁陈博雯张烨覃子海肖玉菲姚瑞玲刘海龙
Owner GUANGXI FORESTRY RES INST
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