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A method for extracting collagen with controllable molecular weight

A collagen and molecular weight technology, applied in the field of collagen extraction, can solve the problems of concentrated molecular weight distribution, low chromium content and high purity of collagen, and achieve the effect of low cost, high purity and simple operation method

Active Publication Date: 2017-10-27
CHINA LEATHER & FOOTWEAR IND RES INST +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] The technical problem to be solved in this application is to provide a method for extracting collagen with controllable molecular weight, so as to solve the problem that the molecular weight distribution of collagen extracted from chrome-containing leather waste is concentrated, the chromium content is low and the purity is high. question

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  • A method for extracting collagen with controllable molecular weight
  • A method for extracting collagen with controllable molecular weight
  • A method for extracting collagen with controllable molecular weight

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0038] A method for extracting collagen from chrome-containing leather waste with controllable molecular weight, specifically:

[0039] (1) Soak chrome-containing leather waste.

[0040] Specifically, 10 g of chrome-containing leather waste was soaked in water.

[0041] In Example 1, chromium-containing leather waste is soaked in water, but in actual production, it is not limited to just soaking in water, and other solvents can also be used.

[0042] (2) Adding an acid agent to make the mixture obtained in the step (1) acidic, and dechroming the mixture obtained in the step (1).

[0043] Specifically, 0.4 g of sulfuric acid was added to the mixture obtained in the step (1), and the mixture obtained in the step (1) was soaked in acidic conditions at room temperature for 10 h to remove the chromium.

[0044] The acid agent used in present embodiment 1 is sulfuric acid, certainly, described acid agent can also be replaced by oxalic acid or hydrochloric acid, perhaps the mixture...

Embodiment 2

[0058] A method for extracting collagen from chromium-containing leather waste with controllable molecular weight, the specific steps are:

[0059] (1) Soak chrome-containing leather waste.

[0060] Specifically, 10 g of chrome-containing leather waste was soaked in water.

[0061] In Example 2, chrome-containing leather waste is soaked in water, but in actual production, it is not limited to just soaking in water, and other solvents can also be used.

[0062] (2) Adding an acid agent to make the mixture obtained in the step (1) acidic, and dechroming the mixture obtained in the step (1).

[0063] Specifically, 0.5 g of hydrochloric acid was added to the mixture obtained in the step (1), and the mixture obtained in the step (1) was soaked in acidic conditions at room temperature for 11 hours to remove the chromium.

[0064] The acid agent that present embodiment 2 adopts is hydrochloric acid, certainly, described acid agent can also be replaced by oxalic acid or sulfuric aci...

Embodiment 3

[0077] A method for extracting collagen with controllable molecular weight, the specific steps are:

[0078] (1) Soak chrome-containing leather waste.

[0079] Specifically, 10 g of chrome-containing leather waste was soaked in water.

[0080] In Example 3, chromium-containing leather waste is soaked in water, but in actual production, it is not limited to just soaking in water, and other solvents can also be used.

[0081] (2) adding an acid agent to make the mixture obtained in the step (1) acidic, and dechroming the mixture obtained in the step (1);

[0082] Specifically, 0.6 g of oxalic acid was added to the mixture obtained in the step (1), and the mixture obtained in the step (1) was soaked and dechromed at room temperature under acidic conditions for 12 hours.

[0083] The acid agent that present embodiment 3 adopts is oxalic acid, certainly, described acid agent can also be replaced by hydrochloric acid or sulfuric acid, perhaps the mixture of above-mentioned three k...

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Abstract

The invention provides a method for extracting collagen with controllable molecular weight, comprising the following steps: (1) soaking chromium-containing leather waste; (2) adding an acid agent to make the mixture obtained in step (1) acidic, And the mixture obtained in step (1) is dechromed; (3) after adding alkaline agent to adjust the pH value to be the optimal pH value of acid protease, hydrolyze the mixture obtained in step (2) with acid protease; (4) Add alkaline agent to adjust the pH value to make the mixture obtained in step (3) alkaline, and dechrome the mixture obtained in step (3); (5) add a compound compound for extracting collagen to the mixture obtained in step (4). Enzyme, the compound enzyme is the complex mixture of papain, bacillus subtilis neutral protease and bacillus licheniformis alkaline protease. The problem that the molecular weight distribution of the currently extracted collagen is not concentrated, the chromium content is high and the purity is low is solved.

Description

technical field [0001] The application relates to the field of comprehensive utilization of waste in leather production, and specifically relates to a method for extracting collagen with controllable molecular weight. Background technique [0002] Although chrome-containing leather waste has a high collagen content (calculated on the basis of dry chrome-containing leather waste, it contains about 90% collagen protein), but because it contains heavy metal chromium, it can only be discarded as garbage or illegally used for a long time. Harm human health or cause serious environmental pollution. In recent years, scholars at home and abroad have done some research on the extraction of collagen from chromium-containing leather waste, and used the extracted collagen in light industry, agriculture, etc., which can reduce the pollution of chromium-containing leather waste, It is also possible to take full advantage of its value. [0003] For the utilization of chromium-containing ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12P21/06
Inventor 丁志文庞晓燕张慧洁刘娜程正平
Owner CHINA LEATHER & FOOTWEAR IND RES INST