United degradation method for dehydrated crude in water by petroleum degradation bacterial flora and surfactant

A technology of surfactants and petroleum degrading bacteria, which is applied in the field of environmental biology, can solve the problems of poisonous microorganisms, low degradation efficiency, and the impact of microbial toxicity, and achieve the effect of simple method and accelerated degradation efficiency

Active Publication Date: 2015-02-18
ZHEJIANG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the efficiency of microorganisms to decompose surfactants by themselves is low, so it is a feasible method to promote their degradation by adding surfactants
There are many types of surfactants on the market, and most of them have toxic effects on microorganisms
For microbial methods with low degradation efficiency, too low surfactant concentration does not even reach the critical micelle concentration and cannot promote repair, while too high surfactant concentration will poison microorganisms

Method used

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  • United degradation method for dehydrated crude in water by petroleum degradation bacterial flora and surfactant
  • United degradation method for dehydrated crude in water by petroleum degradation bacterial flora and surfactant
  • United degradation method for dehydrated crude in water by petroleum degradation bacterial flora and surfactant

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0030] This example uses petroleum hydrocarbon degrading bacteria group and surfactant Tw-80 (c 1 Concentration, c 2 Concentration, c 3 concentration) to jointly degrade dehydrated crude oil, the specific steps are as follows:

[0031] Step (1), enrichment of petroleum hydrocarbon degrading bacteria:

[0032] a: Weigh 1g of petroleum-contaminated soil sample and put it into a 1L Erlenmeyer flask with petroleum hydrocarbon-degrading bacteria culture medium;

[0033] b: The formula of the above-mentioned petroleum hydrocarbon degrading bacteria culture medium is as follows: 65mg K 2 HPO 4 , 25.5mg KH 2 PO 4 , 133.8mg Na 2 HPO 4 12H 2 O, 500mg NH 4 Cl, 82.5mg CaCl 2 , 67.5mg MgSO 4 ·7H 2 O, 0.75mgFeCl 3 ·6H 2 O. Mix with 1000mL deionized water to adjust the pH value to 7.0. Take 100mL of the above-prepared solution and add it to a 1L Erlenmeyer flask, and sterilize at 120°C for 20min. There is the triangular flask of petroleum hydrocarbon degrading bacteria cultur...

Embodiment 2

[0046] In this example, petroleum hydrocarbon degrading bacteria were combined with surfactants Tw-80, SDS, and LAS to degrade dehydrated crude oil. The specific steps are as follows:

[0047] Step (1), enrichment of petroleum hydrocarbon degrading bacteria:

[0048] Step (1) is identical with example 1;

[0049] Step (2), adding different surfactants to combine with petroleum hydrocarbon degrading bacteria to degrade dehydrated crude oil:

[0050] Get 10mL of the culture fluid petroleum hydrocarbon-degrading bacteria culture medium prepared in the above-mentioned step (1) b, and 1mL of the petroleum-hydrocarbon-degrading bacterial colony obtained in the above-mentioned step (1) e, put into a 50mL conical flask; Add 0.01g of dehydrated crude oil to the bottle; then add surfactant Tw-80, SDS, LAS1mL; place in a constant temperature shaking box, keep the temperature at 30°C, and cultivate at a speed of 180r / min to degrade the crude oil in it.

[0051] Erlenmeyer flask is set a...

Embodiment 3

[0058] In this example, oil-degrading bacteria were combined with mixed surfactants (Tw-80+SDS, Tw-80+LAS) to degrade dehydrated crude oil. The specific steps are as follows:

[0059] Step (1), enrichment of petroleum hydrocarbon degrading bacteria:

[0060] Step (1) is identical with example 1;

[0061] Step (2), adding different surfactants to combine with petroleum hydrocarbon degrading bacteria to degrade dehydrated crude oil:

[0062] Get 10mL of the culture fluid petroleum hydrocarbon-degrading bacteria culture medium prepared in the above-mentioned step (1) b, and 1mL of the petroleum-hydrocarbon-degrading bacterial colony obtained in the above-mentioned step (1) e, put into a 50mL conical flask; Add 0.01g of dehydrated crude oil to the bottle; add 1mL of surfactants (Tw-80+SDS, Tw-80+LAS); place in a constant temperature shaking box, keep the temperature at 30°C, and cultivate at a speed of 180r / min. to degrade the crude oil in it.

[0063] Erlenmeyer flask is set a...

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Abstract

The invention relates to the field of environmental biotechnology, and aims to provide a united degradation method for dehydrated crude in water by petroleum degradation bacterial flora and surfactant. The method comprises specific steps as follows: (1), a petroleum-contaminated soil sample is taken and placed in a conical flask filled with a petroleum hydrocarbon degradation bacterium medium to be cultured for 5 days; part of a culture solution is transferred into a new culture medium, raw crude is added, and culture is continued for 5 days; culture is performed repeatedly for 3 times to obtain a petroleum hydrocarbon degradation bacterial flora; and (2) the culture medium and the petroleum hydrocarbon degradation bacterial flora are taken, dehydrated crude and the surfactant are added in sequence, and the added dehydrated crude is degraded through constant-temperature oscillation culture. According to the united degradation method for the dehydrated crude in the water by the petroleum degradation bacterial flora and the surfactant, environmental harm by the stubborn dehydrated crude can be removed in a thorough, efficient and low-cost manner; a bacterial flora enrichment method is simple, convenient and fast, 80% of raw crude can be degraded in 35 days only by the bacterial flora obtained through the method, and compared with degradation only through bacterial flora, the degradation efficiency is doubled through united application of the bacterial flora and the surfactant.

Description

technical field [0001] The invention belongs to the technical field of environmental biology, and in particular relates to a method for jointly degrading dehydrated crude oil by microorganisms and surfactants. Background technique [0002] Petroleum is one of the most important energy sources in modern society, known as "the blood of industry" and "black gold". Crude oil can be refined into various fuels and chemical raw materials, including natural gas, gasoline, kerosene, volatile oil, lubricating oil, paraffin and asphalt. However, during the processing, use and transportation of crude oil, spills and leakage accidents will inevitably occur, resulting in pollution. Petroleum is a complex mixture composed of various hydrocarbons and a small amount of non-hydrocarbon compounds. Crude oil is difficult to degrade and exists in the environment for a long time. There are three types of methods to deal with oil pollution at home and abroad: physical methods, chemical methods,...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): B09C1/10B09C1/08
Inventor 赵和平孟帆温丽莲赖春宇罗一豪陈冉
Owner ZHEJIANG UNIV
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