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A bacterial strain degrading bis(2-ethylhexyl) phthalate and its application in tobacco cultivation

A technology of diphthalate and ethylhexyl, which is applied in the application field of agricultural biotechnology, and can solve the problems that have not yet been reported on the degradable DEHP of Mesophilic Sphingosinobacterium

Active Publication Date: 2017-04-19
ZHENGZHOU TOBACCO RES INST OF CNTC
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0009] But no mesophilic Sphingobacterium ( Sphingobacterium thalpophilum ) research report on degradable DEHP

Method used

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  • A bacterial strain degrading bis(2-ethylhexyl) phthalate and its application in tobacco cultivation
  • A bacterial strain degrading bis(2-ethylhexyl) phthalate and its application in tobacco cultivation

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0022] Will Sphingobacterium thalpophilum Zh-X-08 strain According to 5% inoculum Inoculated on inorganic salt medium (Na 2 HPO 4 12H 2 O 2.0 g, KH 2 PO 4 0.5g, MgSO 4 ·7H 2 O 0.2 g, (NH 4 ) 2 SO 4 1.0 g, 0.05 g of yeast powder, 30 mg / L of bis(2-ethylhexyl) phthalate), cultured at 28 °C for 3 days, added 5 mL of sterile water, and oscillated to obtain a bacterial suspension. The bacterial suspension was inoculated into a 250 mL Erlenmeyer flask containing 30 mL of fermentation medium at a ratio of 5% for fermentation. The culture temperature was 28 °C, the shaker speed was 200 rpm, and the culture time was 72 h. That is, the fermentation broth of strain ZH-X-08 was obtained.

[0023] Composition of fermentation medium: glucose 0.05 g / L, yeast powder 0.05 g / L, KH 2 PO 40.6 g / L, (NH 4 ) 2 SO 4 1.5 g / L, MgSO 4 ·7H 2 O 0.3 g / L, pH 7.5.

[0024] The verification test was carried out in the form of soil potting, and the test was carried out in the greenhouse o...

Embodiment 2

[0027] Will Sphingobacterium thalpophilum Zh-X-08 strain According to 5% inoculum Inoculated on inorganic salt medium (Na 2 HPO 4 12H 2 O 1.5 g, KH 2 PO 4 0.4 g, MgSO 4 ·7H 2 O 0.15 g, (NH 4 ) 2 SO 4 1.2 g, yeast powder 0.04 g, bis(2-ethylhexyl) phthalate 25 mg / L), culture at 28°C for 3 days, add 5 mL of sterile water, and shake to obtain a bacterial suspension. The bacterial suspension was inoculated into a 250 mL Erlenmeyer flask containing 30 mL of fermentation medium at a ratio of 6% for fermentation. The culture temperature was 28 °C, the shaker speed was 200 rpm, and the culture time was 72 h. That is, the fermentation broth of strain ZH-X-08 was obtained.

[0028] Composition of fermentation medium: glucose 0.04 g / L, yeast powder 0.04 g / L, KH 2 PO 4 0.5 g / L, (NH 4 ) 2 SO 4 1.2 g / L, MgSO 4 ·7H 2 O 0.2 g / L, pH 7.5.

[0029] The verification test is carried out in the way of Datian community. It was carried out in the Xiangcheng tobacco area of ​​H...

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Abstract

The invention relates to a bacterial strain for degrading bis(2-ethylhexyl) phthalate and its application in tobacco planting, belonging to the field of agricultural biotechnology applications. The microbial strain of the present invention is a strain of Sphingobacterium thalpophilum zh-x-08, which was deposited in the General Microbiology Center of China Committee for the Collection of Microbial Cultures on August 27, 2013, with a preservation number of CGMCC No.8075 . The bacterial strain of the present invention is fermented to obtain a fermented liquid, and the fermented liquid is added to the tobacco rhizosphere in an appropriate proportion, which can obviously reduce the content of bis(2-ethylhexyl) phthalate, an autotoxic substance in tobacco roots, and promote the growth of tobacco plants .

Description

[0001] Technical field: [0002] The invention relates to a bacterial strain for degrading bis(2-ethylhexyl) phthalate and its application in tobacco planting, belonging to the field of agricultural biotechnology applications. [0003] Background technique: [0004] Di-(2-ethylhexyl) phthalate [di-(2-ethylhexyl) phthalate, DEHP] is the most commonly used type of phthalic acid esters (PAEs), used as a plasticizer Used in the production of polyvinyl chloride resin (PVC). The global annual output of PAEs is about 6 million tons, and the output of DEHP accounts for about (80-85)% of the total PAEs, and the content in end products can reach about 75%. The wide application of plastic products makes PAEs the most common environmental organic pollutant in the world. [0005] The DEHP existing in the environmental medium is very little from nature, and most of it is from the release of human activities. Plastic products release DEHP during use and processing, which can pollute the at...

Claims

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Application Information

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IPC IPC(8): C12N1/20A01P21/00C12R1/01
CPCC09K17/00C09K2101/00C12N1/20C12N1/205C12R2001/01
Inventor 张仕祥奚家勤过伟民王建伟郭建华周汉平薛超群王爱国尹启生张艳玲梁太波
Owner ZHENGZHOU TOBACCO RES INST OF CNTC
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