Method for simultaneously detecting content of multiple phenolic acids in Noni juice by HPLC (high performance liquid chromatography) wavelength switching technology

A technology of wavelength switching and fruit juice, which is applied in the direction of measuring devices, instruments, scientific instruments, etc., to achieve the effect of stable method, accurate calculation and good reproducibility

Active Publication Date: 2015-02-25
CHINA NAT RES INST OF FOOD & FERMENTATION IND CO LTD +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Noni fruit juice is rich in polyphenolic active substances, among which there are many types of phenolic acids. At present, there is no qualitative and quantitative research on various phenolic acids in noni fruit juice

Method used

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  • Method for simultaneously detecting content of multiple phenolic acids in Noni juice by HPLC (high performance liquid chromatography) wavelength switching technology
  • Method for simultaneously detecting content of multiple phenolic acids in Noni juice by HPLC (high performance liquid chromatography) wavelength switching technology
  • Method for simultaneously detecting content of multiple phenolic acids in Noni juice by HPLC (high performance liquid chromatography) wavelength switching technology

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0026] 1. Materials

[0027] 1.1 Instrument

[0028] Thermo UltiMate 3000 high-performance liquid chromatography (DAD-3000 diode array detector, Chromeleon 7 chromatography workstation, Thermo Fisher Company); Thermo Accucore XL C18 (250 mm × 4.6 mm, 4 μm) chromatographic column; rotary evaporator IKA- RV10 (Aika, Germany).

[0029] 1.2 Reagents

[0030] Standard gallic acid, gentisic acid, P-hydroxybenzoic acid, chlorogenic acid, caffeic acid, and ferulic acid were purchased from Beijing Bailingwei Technology Co., Ltd. (purity ≥ 98%). Methanol and glacial acetic acid are chromatographically pure, ethyl acetate is analytically pure, and the water used in the experiment is ultrapure water.

[0031] 1.3 Sample

[0032] Noni Juice is produced by Hainan Noni Biological Engineering Development Co., Ltd., and the product batches are 20121228, 20130105, 20130603, 20140313, 20140325 and 20140326.

[0033] 2. Methods and Results

[0034] 2.1 Preparation of mixed standard solution...

Embodiment 2

[0047] Methodological investigation

[0048] 1. Linear range and detection limit

[0049] Inject 1 μL of the mixed solution of a series of standard products for analysis, repeat the sample injection 3 times for each concentration standard product, draw a standard curve with the peak area y of the standard product against the concentration x (mg / mL), and obtain the regression equation and detection method of the six standard products. See Table 3 for the limit.

[0050] Table 3 Linear range and regression equation of six phenolic acid components

[0051]

[0052] 2. Precision test

[0053] A portion of noni fruit juice (batch number 20140325) was taken for sample preparation. Under the above-mentioned chromatographic conditions, the samples were injected continuously for 6 times, and the mass concentrations of 6 phenolic acids were recorded. The RSD values ​​are shown in Table 4.

[0054] Table 4 Precision measurement results

[0055]

[0056] 3. Reproducibility test ...

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Abstract

The present invention provides a method for simultaneously detecting content of multiple phenolic acids in Noni juice by HPLC (high performance liquid chromatography) wavelength switching technology, the method includes qualitative and quantitative analysis of the phenolic acids in the Noni juice by the HPLC wavelength switching technology, a C18 column is used, a methanol-acetic acid water solution is used as a mobile phase for gradient elution, a detector is a diode array detector, separation and determination of multiple phenolic acid components can be completed simultaneously by wavelength switching, and a standard substance peak area external standard method is used for quantitation. The method is characterized in that by the use of the wavelength switching technology, interfering substances influence is removed, the content of six phenolic acids in the Noni juice can be detected, determinate components can be well separated within 30min, and the method has high accuracy and good repeatability. The method can effectively separate and accurately determine the multiple phenolic acids in Noni the juice, and has very important significance for the research on the efficacy components in the Noni juice and product quality evaluation.

Description

technical field [0001] The invention relates to a detection method for simultaneously measuring the contents of various phenolic acids in noni fruit juice by HPLC wavelength switching technology. Background technique [0002] Noni is a tropical evergreen perennial broad-leaved shrub or small tree, also known as Morinda officinalis, Morinda officinalis, and Luo Li. scientific name Morinda citrifolia Linn . , is a plant of the genus Morinda officinalis of Rubiaceae, native to some Pacific islands, Southeast Asia and Australia, and is now introduced in Hainan Province, my country. Noni fruit is rich in nutritional and medicinal value, known as the "miracle fruit" rich in a large number of active ingredients such as polysaccharides, phenols, flavonoids and iridoids, etc., with anti-tumor, anti-oxidation, immune-enhancing It can prevent and treat various diseases such as strength, blood fat reduction and cardiovascular protection. [0003] Phenolic acids are a class of plant ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N30/88
Inventor 程池陈建国张露李金霞洪启恩谭望桥
Owner CHINA NAT RES INST OF FOOD & FERMENTATION IND CO LTD
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