Unlock instant, AI-driven research and patent intelligence for your innovation.

Method for improving brassica napus microspore cultivation test-tube plantlet field transplanting survival rate

A technology for culturing microspores and cabbage type rapeseed, applied in the field of agricultural science, can solve the problems of low survival rate, affecting the selection and breeding of high-quality rapeseed varieties in the process of genetic breeding and the like, and achieves the effects of good effect, reduced seedling hardening time and simple method.

Inactive Publication Date: 2015-03-11
贵州省油料研究所
View PDF3 Cites 3 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In terms of transplanting microspore-cultured test-tube seedlings of Brassica napus, the survival rate of ordinary microspore-cultured test-tube seedlings is low, which directly affects the genetic breeding process and the selection of high-quality rapeseed varieties

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0016] Embodiment 1 of the present invention: the method for improving Brassica napus microspore culture field transplanting survival rate of test-tube plantlets comprises the following steps:

[0017] 1) Preparation before transplanting: Remove the cover of the test-tube seedlings cultured aseptically 48 hours before transplanting, keep the other environment unchanged, and continue to cultivate for 24 hours; then culture the test-tube seedlings without the cover Place the bottle in a normal temperature environment, in a cool place without direct sunlight, and cultivate overnight for 24 hours. When the leaves start to wither, spray mist water on the leaves. Spray once every 8 hours. If there is no withering phenomenon, do not spray until transplanting;

[0018] 2) Field preparation: Open the field according to the row spacing of 40cm, the nest spacing of 30cm, and 5 nests per row. The base fertilizer is only 45 kg of N / P / K compound fertilizer. Do not use farmyard manure as the ...

Embodiment 2

[0020] Embodiment 2 of the present invention: the method for improving Brassica napus microspore culture field transplanting survival rate of test-tube plantlets comprises the following steps:

[0021] Preparation before transplanting: Uncover the cover of the test-tube seedlings 48 hours before transplanting the sterile-cultured test-tube seedlings, keep the other environment unchanged, and continue to cultivate for 24 hours; To a normal temperature environment, cool and free from hot sun, cultivate overnight for 24 hours, spray mist water to the leaves when the leaves start to wither, spray once every 12 hours, do not spray if there is no withering phenomenon, until transplanting;

[0022] Field preparation: Open the field according to row spacing 40cm, nest spacing 30cm, 5 nests per row, and only apply 45 kg of N / P / K compound fertilizer as base fertilizer. Do not use farmyard manure as base fertilizer. After survival, apply farmyard manure to clear manure water , apply 1000...

Embodiment 3

[0024] Embodiment 3 of the present invention: the method for improving Brassica napus microspore culture field transplanting survival rate of test-tube plantlets comprises the following steps:

[0025] Preparation before transplanting: Uncover the cover of the test-tube seedlings 48 hours before transplanting the sterile-cultured test-tube seedlings, keep the other environment unchanged, and continue to cultivate for 24 hours; To a normal temperature environment, cool and free from hot sun, cultivate overnight for 24 hours, spray mist water to the leaves when the leaves start to wither, spray once every 12 hours, do not spray if there is no withering phenomenon, until transplanting;

[0026] Field preparation: Open the field according to row spacing 40cm, nest spacing 30cm, 5 nests per row, and only apply 45 kg of N / P / K compound fertilizer as base fertilizer. Do not use farmyard manure as base fertilizer. After survival, apply farmyard manure to clear manure water , apply 1000...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention discloses a method for improving brassica napus microspore cultivation test-tube plantlet field transplanting survival rate. The method comprises the steps of preparation before transplanting, field preparation, and transplanting. According to the invention, after brassica napus microspore in-vitro cultivation, seedlings induced from embryoids are subjected to test-tube plantlet transferring; when field transplanting is to be carried out, the test-tube plantlets are directly transplanted into the field. With the method, seedling-hardening time can be reduced, labor cost can be saved, robust seedlings can be provided for transplanting, and seedling survival rate after transplanting can be greatly improved. The method provided by the invention is simple, and has good effect. With the method, rape genetic breeding research and novel variety breeding processes can be promoted.

Description

technical field [0001] The invention relates to the field of agricultural science, in particular to a method for improving the survival rate of Brassica napus microspore cultured test-tube seedlings for field transplantation. Background technique [0002] Brassica napus ( Brassica napus L. ) is the species with the highest grain yield among the three types of oilseed rape (Chinese cabbage-type rape, mustard-type rape, and Brassica napus). It is currently widely planted in the middle and lower reaches of the Yangtze River in my country and is an important oil crop in my country. [0003] Brassica napus is an important oil crop. It can not only produce edible oil, but also its cake is rich in protein and can be used as animal feed. Rapeseed contains a large amount of anti-nutritional substances, such as polyphenolic compounds, lignin and cellulose, among which polyphenolic substances mainly include tannins, proanthocyanidins, flavonoids, derivatives of hydroxyphenylacrylic ac...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Applications(China)
IPC IPC(8): A01G1/00A01H4/00
CPCA01G22/00A01H4/00
Inventor 李超李加纳饶勇杨斌肖华贵王璐璐
Owner 贵州省油料研究所