Unlock instant, AI-driven research and patent intelligence for your innovation.

A method for rapidly producing rotifers and resting eggs thereof

A technology for dormant eggs and rotifers, which is applied in the field of rapid production of rotifers, can solve the problems of rotifer yield stability, difficult to control the maintenance time of the rotifer peak period, and restricting the commercial development of rotifers.

Inactive Publication Date: 2017-01-11
NANJING NORMAL UNIVERSITY
View PDF4 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, in the fishery production, the method of cultivating rotifers mainly adopts the method of pre-fertilizing water quality with organic fertilizers to produce rotifers as biological bait for seedling openings. The stability of rotifers production and the maintenance time of the peak period of rotifers are difficult to control, which restricts the commercialization of rotifers.

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0013] Example 1: Preparation of crucian carp leachate for rapid production of rotifers

[0014] 1. Freshwater carp carp 50kg, rinsed in tap water, put into 100L of aerated tap water, placed in a transparent glass tank, added 4ppm trichlorfon to prevent fly maggots, sealed with a transparent glass cover, at 28-35°C Expose for 20-30 days. Stir 2-3 times a day. After the fish tissue rots and disintegrates naturally, filter through a 200-mesh sieve or sieve, remove the shaped solids, settle for 24 hours, take the supernatant, sterilize at 120°C, let it stand for 12 hours, take the supernatant, and store it at room temperature, Airtight, dark preservation.

[0015] 2. Preparation of rotifer culture solution: take fish extract, and prepare fish extract dilution according to 1:100 (v / v). Add superphosphate 30g / m 3 , ammonium sulfate 10g / m 3 and urea 10g / m 3 .

[0016] 3. Culture of rotifer single "cloning" line: 96-well culture plate, add 1.5ml of rotifer culture solution to ...

Embodiment 2

[0021] Embodiment two: rapid production of resting eggs of rotifers

[0022] 1. Freshwater carp carp 50kg, rinsed in tap water, put into 100L of aerated tap water, placed in a transparent glass tank, added 4ppm trichlorfon to prevent fly maggots, sealed with a transparent glass cover, at 28-35°C Expose for 20-30 days. Stir 2-3 times a day. After the fish tissue rots and disintegrates naturally, filter through a 200-mesh sieve or sieve, remove the shaped solids, settle for 24 hours, take the supernatant, sterilize at 120°C, let it stand for 12 hours, take the supernatant, and store it at room temperature, Airtight, dark preservation.

[0023] 2. Preparation of rotifer culture solution: take fish extract, and prepare fish extract dilution according to 1:100 (v / v). Add superphosphate 30g / m 3 , ammonium sulfate 10g / m 3 and urea 10g / m 3 .

[0024] 3. Culture of rotifer single "cloning" line: 96-well culture plate, add 1.5ml of rotifer culture solution to each well, and the d...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention relates to a method for rapidly producing freshwater wheel animalcule and resting eggs of the freshwater wheel animalcule by utilizing a leaching solution of freshwater fishes. According to the method, the leaching solution of fishes is utilized for rapidly producing the freshwater water wheel animalcule and resting eggs of the freshwater wheel animalcule; the leaching solution of the fishes is filtrate obtained after freshwater fish bodies or internal organ wastes are naturally decomposed and degraded by aerated tap water. The leaching solution of the fishes is artificially prepared by adopting the aerated tap water under natural light illumination and room temperature; bait micro-algae and a wheel animalcule solution universal culture medium are prepared according to the ratio of the leaching solution, and the wheel animalcule take freshwater chlorella as a food. The freshwater wheel animalcule and the resting eggs thereof are rapidly produced by adopting a continuous air inflation and continuous illumination method. The method can be used for conveniently, rapidly and economically collecting lots freshwater wheel animalcule and the resting eggs of the wheel animalcule for fish initial feeding baits and other researches. The method is applicable to lab researches and production of biological baits for initial feeding of aquatic product products. The method is simple, convenient, rapid, economical and convenient to use.

Description

technical field [0001] The invention belongs to the field of biological technology of aquatic science, and in particular relates to a method for rapidly producing rotifers by using freshwater fish corpses or their viscera to prepare leachate. Background technique [0002] Rotifer (rotifer) is the main functional group of freshwater ecosystems, and more than 95% of fishes and shrimps and crabs feed early in the seedling period, and they are also the most suitable test animals for environmental pollution risk assessment. At present, rotifers are mainly cultivated in fishery production by using organic fertilizers to fertilize water quality in advance to produce rotifers as biological bait for seedling openings. It is difficult to control the stability of rotifers production and the maintenance time of rotifers peak period, which restricts the commercialization of rotifers. The present invention uses freshwater fish carcasses or their visceral wastes to prepare leaching liquid ...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Patents(China)
IPC IPC(8): A01K67/033A23K10/20A23K50/90
CPCA01K67/033A23K10/22A23K50/80
Inventor 杨家新
Owner NANJING NORMAL UNIVERSITY