Preparation method of collagen membrane and collagen membrane thereof

A collagen membrane and collagen technology, which is applied in the preparation of collagen membranes and in the field of collagen membranes, can solve the problems of loose collagen membranes and not suitable for the protective layer of the dermis, and achieve the effect of avoiding wound infection.

Active Publication Date: 2015-03-11
陕西艾尔肤组织工程有限公司
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  • Abstract
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  • Application Information

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Problems solved by technology

[0007] The collagen membrane disclosed in the prior art is directly formed into a membrane by centrifugation, and the obtained collagen membrane is relat

Method used

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  • Preparation method of collagen membrane and collagen membrane thereof
  • Preparation method of collagen membrane and collagen membrane thereof
  • Preparation method of collagen membrane and collagen membrane thereof

Examples

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preparation example Construction

[0039] For a preparation method of collagen membrane provided by an embodiment of the present invention, see figure 1 ,include:

[0040] Step 1. Prepare a collagen solution, the pH of the collagen solution is 6-8;

[0041] Step 2. Gel the collagen solution to obtain a gel mass;

[0042] Step 3. Vibrate, crosslink and clean the rubber block under the action of a crosslinking agent;

[0043] Step 4. Press the gel block after shaking, cross-linking and washing to obtain a collagen film.

[0044] Through the above steps 1 and 4, the obtained collagen membrane is relatively dense and has suitable degradability, which can effectively protect the dermal matrix layer and avoid wound infection.

Embodiment 1

[0073] Prepare collagen solution: mix collagen with 0.05% acetic acid; put it in a refrigerator at 4°C for 48 hours to dissolve completely; add phenol red solution to the dissolved collagen solution under ice bath conditions, among which, collagen The volume ratio of protein solution to phenol red solution is 100:1, and the pH value is adjusted to 6.5 with NaOH;

[0074] Gel: Put the configured collagen solution into a gel mold, and gel at 37°C for 60 minutes to obtain a gel block;

[0075] Cross-linking: cross-link the gel block obtained from the gel under the action of 50mM EDC, shaking at room temperature for 16h.

[0076] Film pressing: press the rubber block at a pressure of 1.0 kg / block for 10 minutes, and then press the block at a pressure of 2.5 kg / block for 60 minutes; wash the block with purified water 5 times; Then, press the cleaned rubber block again for 30 minutes at a pressure of 3 kg / rubber block.

[0077] The thickness of the collagen membrane obtained in this exampl...

Embodiment 2

[0079] Configure collagen solution: mix collagen with 0.1% acetic acid; put it in a refrigerator at 6°C for 72 hours to dissolve completely; add phenol red solution to the dissolved collagen solution under ice bath conditions, among which, collagen The volume ratio of the solution to the phenol red solution is 100:2, and the pH value is adjusted to 7 with NaOH;

[0080] Gel: Put the configured collagen solution into a gel mold, and gel at 37°C for 50 minutes to obtain a gel block;

[0081] Cross-linking: cross-link the gel block obtained by gel under the action of 30mM EDC, shaking at room temperature for 36h.

[0082] Film pressing: press the rubber block at a pressure of 1.2kg / block for 12 minutes, then press the block at a pressure of 3kg / block for 60 minutes; wash the block with purified water 4 times; then According to the pressure of 5kg / rubber block, press the cleaned rubber block again for 20 minutes.

[0083] The thickness of the collagen membrane obtained in this example is...

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Abstract

The invention relates to a preparation method of a collagen membrane and the collagen membrane thereof, which belongs to the biology field. The collagen membrane has the compact performance and appropriate degradability, and can effectively protect an acellular dermal matrix layer and avoid wound infection. The membrane comparison overcomes the defects of rarefaction and improper usage as a protection layer for the corium layer of tissue engineering skin in prior art. The preparation method of the collagen membrane comprises the following steps: preparing a collagen solution, wherein pH value of the collagen solution is 68-; gelling the collagen solution to obtain collagen block; concussing and crosslinking the collagen block under effect of a cross-linking agent, and then cleaning; and pressing the cleaned collagen block to obtain the collagen membrane.

Description

Technical field [0001] The invention relates to the field of biology, in particular to a method for preparing a collagen membrane and a collagen membrane. Background technique [0002] The skin is the largest organ of the human body. It has the functions of barrier, sensation, regulation of body temperature, secretion and excretion, participation in immune response, and maintenance of internal and external environment stability. With the development of economy, technology and society and the extension of the average life span of human beings, skin defects caused by various traumas, especially burns and senile diseases, are increasing. Autologous skin transplantation, as the gold standard for the treatment of such skin defects, has achieved great results in clinical applications. However, autologous skin transplantation requires surgical cutting of the patient’s intact skin, and then covering the patient’s skin defect area, which intensifies the blow to the patient, and may form ...

Claims

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Application Information

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IPC IPC(8): A61L27/24A61L27/38A61L27/60A61L27/54
Inventor 杨苹杨维荣辉张爱兵
Owner 陕西艾尔肤组织工程有限公司
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