Preparation method of collagen membrane and collagen membrane thereof
A collagen membrane and collagen technology, which is applied in the preparation of collagen membranes and in the field of collagen membranes, can solve the problems of loose collagen membranes and not suitable for the protective layer of the dermis, and achieve the effect of avoiding wound infection.
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[0039] For a preparation method of collagen membrane provided by an embodiment of the present invention, see figure 1 ,include:
[0040] Step 1. Prepare a collagen solution, the pH of the collagen solution is 6-8;
[0041] Step 2. Gel the collagen solution to obtain a gel mass;
[0042] Step 3. Vibrate, crosslink and clean the rubber block under the action of a crosslinking agent;
[0043] Step 4. Press the gel block after shaking, cross-linking and washing to obtain a collagen film.
[0044] Through the above steps 1 and 4, the obtained collagen membrane is relatively dense and has suitable degradability, which can effectively protect the dermal matrix layer and avoid wound infection.
Embodiment 1
[0073] Prepare collagen solution: mix collagen with 0.05% acetic acid; put it in a refrigerator at 4°C for 48 hours to dissolve completely; add phenol red solution to the dissolved collagen solution under ice bath conditions, among which, collagen The volume ratio of protein solution to phenol red solution is 100:1, and the pH value is adjusted to 6.5 with NaOH;
[0074] Gel: Put the configured collagen solution into a gel mold, and gel at 37°C for 60 minutes to obtain a gel block;
[0075] Cross-linking: cross-link the gel block obtained from the gel under the action of 50mM EDC, shaking at room temperature for 16h.
[0076] Film pressing: press the rubber block at a pressure of 1.0 kg / block for 10 minutes, and then press the block at a pressure of 2.5 kg / block for 60 minutes; wash the block with purified water 5 times; Then, press the cleaned rubber block again for 30 minutes at a pressure of 3 kg / rubber block.
[0077] The thickness of the collagen membrane obtained in this exampl...
Embodiment 2
[0079] Configure collagen solution: mix collagen with 0.1% acetic acid; put it in a refrigerator at 6°C for 72 hours to dissolve completely; add phenol red solution to the dissolved collagen solution under ice bath conditions, among which, collagen The volume ratio of the solution to the phenol red solution is 100:2, and the pH value is adjusted to 7 with NaOH;
[0080] Gel: Put the configured collagen solution into a gel mold, and gel at 37°C for 50 minutes to obtain a gel block;
[0081] Cross-linking: cross-link the gel block obtained by gel under the action of 30mM EDC, shaking at room temperature for 36h.
[0082] Film pressing: press the rubber block at a pressure of 1.2kg / block for 12 minutes, then press the block at a pressure of 3kg / block for 60 minutes; wash the block with purified water 4 times; then According to the pressure of 5kg / rubber block, press the cleaned rubber block again for 20 minutes.
[0083] The thickness of the collagen membrane obtained in this example is...
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