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A kind of preparation method of collagen film and collagen film

A collagen film and collagen technology, which is applied in the preparation of collagen film and in the field of collagen film, can solve the problems of loose collagen film and unsuitable dermis protective layer, etc., and achieve the effect of avoiding wound infection.

Active Publication Date: 2016-08-17
陕西艾尔肤组织工程有限公司
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] The collagen membrane disclosed in the prior art is directly formed into a membrane by centrifugation, and the obtained collagen membrane is relatively loose, which is suitable for guiding tissue regeneration, but is not suitable for use as a protective layer of the dermis of tissue-engineered skin

Method used

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  • A kind of preparation method of collagen film and collagen film
  • A kind of preparation method of collagen film and collagen film
  • A kind of preparation method of collagen film and collagen film

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preparation example Construction

[0039] A kind of preparation method of collagen membrane provided by the embodiment of the present invention, see figure 1 ,include:

[0040] Step 1, configure collagen solution, the pH value of the collagen solution is 6-8;

[0041] Step 2, gelling the collagen solution to obtain a gel block;

[0042] Step 3, shaking and cross-linking the rubber block under the action of a cross-linking agent and cleaning;

[0043] Step 4. Pressing the vibrated, cross-linked and washed rubber block into a film to obtain a collagen film.

[0044] Through the above steps 1 and 4, the obtained collagen film is relatively dense and has suitable degradability, which can effectively protect the dermal matrix layer and avoid wound infection.

Embodiment 1

[0073] Collagen solution preparation: mix collagen with 0.05% acetic acid; put it in a refrigerator at 4°C for 48 hours to dissolve completely; add phenol red solution to the dissolved collagen solution under ice bath conditions, among which collagen The volume ratio of the protein solution to the phenol red solution is 100:1, and the pH value is adjusted to 6.5 with NaOH;

[0074] Gel: Put the configured collagen solution into the gel mold, and gel at a constant temperature of 37°C for 60 minutes to obtain gel blocks;

[0075] Cross-linking: under the action of EDC at a concentration of 50 mM, the gel blocks obtained from the gel were shaken and cross-linked at room temperature for 16 hours.

[0076] Film pressing: pressurize the rubber block for 10 minutes at a pressure of 1.0 kg / block, and then pressurize the block for 60 minutes at a pressure of 2.5 kg / block; wash the block with purified water for 5 times; Then according to the pressure of 3kg / rubber block, pressurize the...

Embodiment 2

[0079] Collagen solution preparation: mix collagen with 0.1% acetic acid; put it in a refrigerator at 6°C for 72 hours, and dissolve completely; add phenol red solution to the dissolved collagen solution in an ice bath, wherein collagen The volume ratio of the solution to the phenol red solution is 100:2, and the pH value is adjusted to 7 with NaOH;

[0080] Gel: Put the configured collagen solution into the gel mold, and gel at a constant temperature of 37°C for 50 minutes to obtain gel blocks;

[0081] Cross-linking: under the action of EDC with a concentration of 30 mM, the gel blocks obtained from the gel were shaken and cross-linked at room temperature for 36 hours.

[0082] Film pressing: pressurize the rubber block for 12 minutes at a pressure of 1.2 kg / block, and then pressurize the block for 60 minutes at a pressure of 3 kg / block; wash the block with purified water 4 times; then According to the pressure of 5kg / rubber block, it is enough to pressurize the cleaned rub...

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Abstract

The invention relates to a preparation method of a collagen membrane and the collagen membrane thereof, which belongs to the biology field. The collagen membrane has the compact performance and appropriate degradability, and can effectively protect an acellular dermal matrix layer and avoid wound infection. The membrane comparison overcomes the defects of rarefaction and improper usage as a protection layer for the corium layer of tissue engineering skin in prior art. The preparation method of the collagen membrane comprises the following steps: preparing a collagen solution, wherein pH value of the collagen solution is 68-; gelling the collagen solution to obtain collagen block; concussing and crosslinking the collagen block under effect of a cross-linking agent, and then cleaning; and pressing the cleaned collagen block to obtain the collagen membrane.

Description

technical field [0001] The invention relates to the field of biology, in particular to a method for preparing a collagen film and the collagen film. Background technique [0002] The skin is the largest organ of the human body. It has functions such as barrier, sensation, temperature regulation, secretion and excretion, participation in immune response, and maintenance of internal and external environment stability. With the development of economy, technology and society and the prolongation of the average life expectancy of human beings, there are more and more skin defects caused by various traumas, especially burns and senile diseases. As the gold standard for the treatment of such skin defects, autologous skin transplantation has achieved great results in clinical application. However, autologous skin transplantation needs to cut off the patient's intact skin through surgery, and then cover the patient's skin defect area, which intensifies the blow to the patient, and m...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61L27/24A61L27/38A61L27/60A61L27/54
Inventor 杨苹杨维荣辉张爱兵
Owner 陕西艾尔肤组织工程有限公司
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