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Method and system for identifying histologic origin of body fluids of Chinese population from gene level

A Chinese, body fluid technology, applied in biochemical equipment and methods, microbial determination/inspection, etc., can solve the problems of different specific gene expression abundance, inability to obtain results, difficult identification, etc., to achieve the effect of efficient utilization

Active Publication Date: 2015-03-18
INST OF FORENSIC SCI OF MIN OF PUBLIC SECURITY
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Problems solved by technology

[0004] Therefore, researchers at home and abroad have done a lot of research on determining the tissue sources of body fluids or their spots from the genetic level, but there is no report in China that shows that the above peripheral blood, menstrual blood, saliva, vaginal secretions and Accurate and rapid identification of semen. Although foreign countries have reported identification methods for various body fluids and have begun to try forensic applications, when using their identification methods to identify the above-mentioned body fluids in the Chinese population, the results are often unable to be obtained, or obtained. The results of the situation are opposite. The possible reasons for these situations are that the specific genes contained in the body fluids of different regions and races are different, and the expression abundance of these specific genes is different. It is difficult for foreign research results to be directly applied to China. Identification of Tissue Sources of Body Fluids in Populations

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  • Method and system for identifying histologic origin of body fluids of Chinese population from gene level
  • Method and system for identifying histologic origin of body fluids of Chinese population from gene level
  • Method and system for identifying histologic origin of body fluids of Chinese population from gene level

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Embodiment 1

[0062] Embodiment 1, verify the accuracy of the system and method of the present invention

[0063] In this embodiment, the above five kinds of simulated body fluid samples are detected separately or in combination using the method and system of the present invention.

[0064] The system for identifying the source of Chinese population fluid tissue from the gene level of the present invention includes an RNA extraction system, a reverse transcription system, a PCR amplification system and a detection amplification product system. The system is used to detect the expression status of seven genes GYPA, SPTB, MMP-7, STATH, MUC4, PRM2 and TGM4 in body fluids, and the body fluids include one of peripheral blood, menstrual blood, saliva, vaginal secretions and semen. One or more, the body fluid with GYPA and / or SPTB gene expression is the body fluid containing peripheral blood; the body fluid with at least one expression of GYPA and SPTB gene, and the expression of MMP-7 gene is the...

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Abstract

The invention provides a method and a system for identifying a histologic origin of body fluids of Chinese population from gene level. The method comprises the following step: detecting the expression status of seven genes: GYPA, SPTB, MMP-7, STATH, MUC4, PRM2 and TGM4 in body fluids, wherein the body fluids comprise one or more of peripheral blood, menstrual blood, saliva, vaginal secretion and seminal fluid. The invention further provides the system for identifying the histologic origin of body fluids of Chinese population from gene level. According to the scheme provided by the invention, the histologic origin of body fluids of Chinese population can be effectively determined from the gene level, so that steps of identifying the histologic origin of body fluids in criminal investigation fields or spots thereof are greatly simplified, and an accurate scientific basis is provided for defining case property, determining criminal suspects, convicting and sentencing and the like.

Description

technical field [0001] The present invention relates to a method and system for the source of human body fluid tissue, in particular to a method and system for identifying the source of Chinese body fluid tissue from the gene level. Background technique [0002] After nearly 30 years of development in the field of forensic DNA in my country, a large number of research results including domestically produced DNA reagents have been obtained, and related technologies have become an important scientific and technological support in the practice of public security cases. The detection and identification of bodily fluids (including peripheral blood, menstrual blood, saliva, vaginal secretions and semen, etc.) and their spots or traces left at crime scenes is an important link in forensic identification. [0003] Identifying the tissue source of bodily fluids can provide decisive evidence for on-site reconstruction, determine the nature of the case, and provide key clues for solvin...

Claims

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Application Information

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IPC IPC(8): C12Q1/68
CPCC12Q1/6888C12Q2600/158
Inventor 王冲李万水胡兰徐秀兰石屹凃政
Owner INST OF FORENSIC SCI OF MIN OF PUBLIC SECURITY
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