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Method for extracting total eleutheroside

A technology of total glucosides and extraction methods of Acanthopanax senticosus, applied in the direction of medical formulas, medical preparations containing active ingredients, plant raw materials, etc., to achieve the effects of improving purity, solvent consumption, less waste generation, and fast extraction speed

Inactive Publication Date: 2015-03-25
通化金汇生物工程有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, the roots and stems of Acanthopanax senticosus are used as medicinal materials, which are similar to ginseng. There is no report on the purification and utilization of Acanthopanax senticosus leaves

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0020] A method for extracting total glycosides of Acanthopanax senticosus, comprising the following preparation steps:

[0021] (1) After pulverizing the leaves of Acanthopanax senticosus and immersing them in ethanol with a concentration of 75%, under the environment of 600KPa, carry out microwave extraction to obtain the extract; the microwave extraction time is 8 minutes; the Acanthopanax senticosus and The mass ratio of ethanol to liquid is 1:8.

[0022] (2) After filtering the extract that step (1) obtains, adjust the pH with citric acid to be 2, and then adsorb through a weakly polar macroporous adsorption resin chromatography column; the macroporous adsorption resin is Anhui Samsung Resin Technology Co., Ltd. : HPD 100 macroporous resin.

[0023] (3) eluting the resin column with a first solvent; the first solvent is ethanol with a concentration of 5%;

[0024] (4) elute the resin column again with a second solvent; the second solvent is ethanol with a concentration ...

Embodiment 2

[0026] A method for extracting total glycosides of Acanthopanax senticosus, comprising the following preparation steps:

[0027] (1) After pulverizing the leaves of Acanthopanax senticosus and immersing them in ethanol with a concentration of 80%, under the environment of 800KPa, carry out microwave extraction to obtain the extract; the microwave extraction time is 10 minutes; the Acanthopanax senticosus and The mass ratio of ethanol to liquid was 1:30.

[0028] (2) After filtering the extract obtained in step (1), the pH is adjusted to 6 with citric acid, and then adsorbed by a nonpolar macroporous adsorption resin chromatography column;

[0029] (3) eluting the resin column with a first solvent; the first solvent is ethanol with a concentration of 20%;

[0030] (4) elute the resin column again with a second solvent; the second solvent is ethanol with a concentration of 80%; take the fraction and concentrate it into a thick paste to obtain eleutherosides, and use ultraviolet...

Embodiment 3

[0032] A method for extracting total glycosides of Acanthopanax senticosus, comprising the following preparation steps:

[0033] (1) After pulverizing the leaves of Acanthopanax senticosus and immersing them in ethanol with a concentration of 77%, under the environment of 700KPa, carry out microwave extraction to obtain the extract; the microwave extraction time is 12 minutes; the Acanthopanax senticosus and The mass ratio of ethanol to liquid is 1:20.

[0034] (2) After filtering the extract obtained in step (1), the pH is adjusted to 5 with citric acid, and then adsorbed by a weakly polar macroporous adsorption resin chromatography column;

[0035] (3) eluting the resin column with a first solvent; the first solvent is ethanol with a concentration of 10%;

[0036] (4) elute the resin column again with a second solvent; the second solvent is ethanol with a concentration of 77%; take the fraction and concentrate it into a thick paste to obtain eleutherosides, and use ultravio...

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PUM

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Abstract

The invention discloses a method for extracting total eleutheroside. Microwave extraction is adopted in the method, and the microwave extraction time is 8-12 minutes; the extraction quantity of the total eleutheroside can be improved by adopting the method for extracting the total eleutheroside, and is generally 20-55wt% in the prior art but 50-80wt% in the method; the consumption of a solvent and the production of wastes are low, so the method meets the requirements of green chemistry and environmental protection; and the purity of the extract obtained by adopting the method is high.

Description

technical field [0001] The invention relates to the technical field of traditional Chinese medicine extraction. More specifically, it relates to a method for extracting total eleutherosides. Background technique [0002] Acanthopanax is one of the important medicinal plants of the genus Acanthopanax of Araliaceae, mainly distributed in Jilin, Heilongjiang, Liaoning and other places in my country. my country's Acanthopanax is rich in resources, especially in the Changbai Mountain area, because of the unique regional ecological conditions, it provides a good natural environment for the growth of wild Acanthopanax. The fruit of Acanthopanax senticosus is the top grade in traditional Chinese medicine, and it is also the third-generation fruit. It is rich in nutrients and pharmacological activities. Studies have found that the fruit of Acanthopanax senticosus contains saponins, carotene glycosides and other biologically active components. In recent years, saponins have attracte...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K36/254
CPCA61K36/254A61K2236/333A61K2236/37
Inventor 张强张鹏
Owner 通化金汇生物工程有限公司
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