Method for extracting alkali-soluble polysaccharides from sparassis crispa and application of alkali-soluble polysaccharides to age delaying

An extraction method, hydrangea technology, applied in the field of biochemistry, can solve the problem of rare anti-aging activity of alkali-soluble polysaccharides, and achieve the effect of life extension

Inactive Publication Date: 2015-03-25
DAQING NORMAL UNIV
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Polysaccharide is an important active ingredient of hydrangea, which has pharmacological activities such as immunoregulation, anti-tumor,

Method used

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  • Method for extracting alkali-soluble polysaccharides from sparassis crispa and application of alkali-soluble polysaccharides to age delaying
  • Method for extracting alkali-soluble polysaccharides from sparassis crispa and application of alkali-soluble polysaccharides to age delaying
  • Method for extracting alkali-soluble polysaccharides from sparassis crispa and application of alkali-soluble polysaccharides to age delaying

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Embodiment

[0013] 1. Extraction method of hydrangea alkali-soluble polysaccharide

[0014] The method comprises the steps of:

[0015] (1) Alkali-soluble crude polysaccharide extraction

[0016] Hydrangea sporocarp powder was swollen with water overnight, extracted in a water bath three times for 2 hours each time, and then centrifuged. The resulting residue was broken by a cell disruptor for 10 minutes, extracted with 0.1mol / mL sodium hydroxide three times for 2 hours each time, and then centrifuged again, and the supernatant was combined. solution, neutralized with 0.1mol / mL hydrochloric acid and then concentrated to 10mL, the concentrated solution was alcohol-precipitated with 3 times the volume of absolute ethanol and centrifuged overnight, and then added absolute ethanol and diethyl ether to the precipitate for washing, and after centrifugation, the precipitate was dry, that is;

[0017] (2) Purification of alkali-soluble crude polysaccharide

[0018] Accurately weigh the alkali-...

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Abstract

The invention discloses a method for extracting alkali-soluble polysaccharides from sparassis crispa and application of the alkali-soluble polysaccharides to age delaying, and belongs to the field of biological chemistry. The method comprises the steps of firstly, extracting crude polysaccharides from sparassis crispa fruiting body powder by using thin alkali; then, preparing 5% sugar liquid by using distilled water; adding 10% trichloroacetic acid to regulate the pH value; and standing, centrifuging, carrying out alcohol precipitation and cleaning to obtain deproteinized alkali-soluble polysaccharides. The research on the influences of the alkali-soluble polysaccharides in sparassis crispa to the service life of fruit flies proves that both the crude alkali-soluble polysaccharides in sparassis crispa and the deproteinized alkali-soluble polysaccharides which have different concentration values have a role in prolonging the service life of the fruit flies and have an age delaying effect; in addition, the alkali-soluble polysaccharides has more obvious effect on the service life prolonging effect on male fruit flies than that on female fruit flies.

Description

technical field [0001] The invention relates to the field of biochemistry, in particular to an extraction method of hydrangea alkali-soluble polysaccharide and its anti-aging activity application. Background technique [0002] Hydrangea (Sparassis crispa Fr.), which belongs to the subclass of Hydrangea, Hydrangea, Hydrangea, Hydrangea, its fruit body is white and tender, delicious, and rich in nutrition. It is a very rare and precious species. Edible and medicinal fungi. Polysaccharide is an important active ingredient of hydrangea, which has pharmacological activities such as immunoregulation, anti-tumor, hypoglycemic, and blood fat reduction. However, research on the anti-aging activity of hydrangea alkali-soluble polysaccharides is rare. Contents of the invention [0003] In order to solve the problems of the prior art, the embodiment of the present invention provides a method for extracting alkali-soluble polysaccharide from Hydrangea hydrangea and its anti-aging acti...

Claims

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Application Information

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IPC IPC(8): C08B37/00A61P39/00
Inventor 张丽霞武燕卞立红张志国兰天丽
Owner DAQING NORMAL UNIV
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