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Preparation method of novel hog cholera vaccine containing TH polypeptide

A swine fever vaccine, a new technology, applied in the field of design and development of biological vaccines

Inactive Publication Date: 2015-04-01
TECON BIOLOGY CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

There are no institutions and scholars in China to add TH polypeptide to the E2 protein of classical swine fever virus for the development of vaccines

Method used

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  • Preparation method of novel hog cholera vaccine containing TH polypeptide
  • Preparation method of novel hog cholera vaccine containing TH polypeptide
  • Preparation method of novel hog cholera vaccine containing TH polypeptide

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0008] Embodiment 1, a preparation method of a novel swine fever vaccine containing TH polypeptide, the method comprises the following steps: Step 1: The classical swine fever virus E2 protein and TH polypeptide form an antigen solution according to the ratio of 1~4:1~3 , standby; step 2: the antigen solution prepared in step 1 and the 563VG adjuvant are prepared in a mass ratio of 1:1 to make a mixture of the antigen solution and the adjuvant, using a homogenizer, under the condition of 12000-15000rpm, mix the The liquid is homogeneous, and the time is 10-15min, that is, a novel swine fever vaccine containing TH polypeptide is made; wherein, the swine fever virus E2 protein described in step 1 is shown in SEQ ID NO.1, according to conventionally known biological method; the TH polypeptide shown in SEQ ID NO.2 is prepared according to conventionally known biological methods; the 563VG adjuvant described in step 2 is a commercially available product.

[0009] Specific practice...

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Abstract

The invention belongs to the technical fields of design and development of biological vaccines and particularly relates to a preparation method of a novel hog cholera vaccine containing TH polypeptide. The method comprises the following steps: 1 preparing an antigen liquid by using hog cholera virus E2 protein and the TH polypeptide at the ratio of (1-4) to (1-3) for later use; and 2 preparing a mixed liquid of the antigen liquid and an adjuvant by using the antigen liquid prepared in the step 1 and the 563VG adjuvant at the mass ratio of 1 to 1, and homogenizing the mixed liquid for 10-15 minutes at the speed of 12,000-15,000rpm by virtue of a homogenizer, so as to prepare the novel hog cholera vaccine containing the TH polypeptide. The method is mainly applied to prevention and immunization of the hog cholera virus.

Description

technical field [0001] The invention belongs to the technical field of design and development of biological vaccines, in particular to a preparation method of a novel swine fever vaccine containing TH polypeptide. Background technique [0002] The current CSF vaccines are CSF Lapinized Attenuated Vaccine and CSFV E2 Recombinant Baculovirus Inactivated Vaccine. The attenuated CSF vaccine has good immunogenicity, but it cannot be distinguished whether it is the antibody produced by the immune vaccine or the antibody produced by the infected wild virus during clinical use, and it cannot be differentially diagnosed and purified from CSFV; CSFV The E2 recombinant baculovirus inactivated vaccine also has good immunogenicity. After immunizing pigs, antibody detection can be used to distinguish the antibodies produced by vaccine immunization from the antibodies produced by wild virus infection, which can be used for differential diagnosis; but the recombinant swine fever virus E2 T...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K39/187A61K39/00A61P31/14
Inventor 李俊辉赵毅
Owner TECON BIOLOGY CO LTD