Method for extracting laminarin from kelp
A technology of kelp polysaccharide and extraction method, which is applied in the field of polysaccharide extraction, can solve the problems that restrict the deep processing and application of kelp polysaccharide, and achieve the effects of shortening the extraction time, maturing the process, and promoting dissolution
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Embodiment 1
[0018] A method for extracting laminarin, comprising the steps of:
[0019] 1) Raw material processing: 10 kg of dried sea-tangle is pulverized;
[0020] 2) Ultra-high pressure treatment: add 400kg of pure water to the pulverized kelp, pressure 350MPa, temperature 85°C, ultra-high pressure treatment for 4 minutes, filter after completion, and collect the filtrate;
[0021] 3) Ultrasonic treatment: The filtrate obtained in step 2) was ultrasonically treated for 10 minutes at an operating temperature of 85°C and a power of 300W. After completion, filter and collect the filtrate;
[0022] 4) Protein removal and drying: after the filtrate obtained in step 3) is concentrated, the protein is removed by the Sevag method, the upper layer solution is collected, and laminarin is obtained after freeze-drying.
Embodiment 2
[0024] A method for extracting laminarin, comprising the steps of:
[0025] 1) Raw material processing: crush 10kg of fresh kelp;
[0026] 2) Ultra-high pressure treatment: add 600kg of pure water to the crushed kelp, pressure 350MPa, action temperature 80°C, ultra-high pressure treatment for 2min, filter after completion, and collect the filtrate;
[0027] 3) Ultrasonic treatment: The filtrate obtained in step 2) was ultrasonically treated for 8 minutes at an operating temperature of 80°C and a power of 300W. After completion, filter and collect the filtrate;
[0028] 4) Protein removal and drying: after the filtrate obtained in step 3) is concentrated, the protein is removed by the Sevag method, the upper layer solution is collected, and laminarin is obtained after freeze-drying.
Embodiment 3
[0030] A method for extracting laminarin, comprising the steps of:
[0031] 1) Raw material processing: 10 kg of dried sea-tangle is pulverized;
[0032] 2) Ultra-high pressure treatment: Add 500kg of pure water to the pulverized kelp, pressure 350MPa, temperature 70°C, ultra-high pressure treatment for 5 minutes, filter after completion, and collect the filtrate;
[0033] 3) Ultrasonic treatment: process the filtrate obtained in step 2) for 5 minutes with ultrasonic treatment at a temperature of 70° C. and a power of 300 W. After completion, filter and collect the filtrate;
[0034] 4) Protein removal and drying: after the filtrate obtained in step 3) is concentrated, the protein is removed by the Sevag method, the upper layer solution is collected, and laminarin is obtained after freeze-drying.
[0035] result:
[0036]
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