A kind of preparation method of heavy metal lead polluted soil leachate

A technology for lead-contaminated soil and eluent, applied in the field of soil remediation, can solve the problems of complicated preparation methods, high preparation costs, and low leaching rate

Inactive Publication Date: 2019-03-01
YUNNAN MINZU UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the preparation cost of heavy metal-contaminated soil eluent is often too high, the preparation method is complicated, and the leaching rate is low

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0018] A kind of preparation method of heavy metal lead polluted soil eluent proposed by the present invention comprises the following steps:

[0019] S1, take the inclined-plane PDA containing Aspergillus niger, rinse the surface of the inclined-plane PDA with sterile water to obtain the first mixed material, place the first mixed material in a sterile Erlenmeyer flask, and vibrate at a speed of 145rpm for 13h to obtain black Aspergillus liquid, culture temperature is 28 ℃;

[0020] S2. Pulverize the tea seed cake to a particle size of 2-3 mm, add water and mix it, then sterilize it at high temperature, add Aspergillus niger liquid after cooling to room temperature, place it on a constant temperature shaking table and cultivate it for 115 hours to obtain the second mixed material, the cultivation temperature of the constant temperature shaking table The temperature is 30°C, and the speed of the constant temperature shaker is 145rpm;

[0021] S3. After the second mixed materi...

Embodiment 2

[0023] A kind of preparation method of heavy metal lead polluted soil eluent proposed by the present invention comprises the following steps:

[0024] S1. Take the inclined-plane PDA containing Aspergillus niger, rinse the surface of the inclined-plane PDA with sterile water to obtain the first mixed material, place the first mixed material in a sterile Erlenmeyer flask, and vibrate at a speed of 155 rpm for 11 hours to obtain black Aspergillus liquid, culture temperature is 30 ℃;

[0025] S2. Pulverize the tea seed cake to a particle size of 2-3mm, add water and mix it, then sterilize it at high temperature, add Aspergillus niger liquid after cooling to room temperature, place it on a constant temperature shaking table and cultivate it for 125 hours to obtain the second mixed material, the cultivation temperature of the constant temperature shaking table The temperature is 28°C, and the rotation speed of the constant temperature shaker is 155rpm;

[0026] S3. After the secon...

Embodiment 3

[0028] A kind of preparation method of heavy metal lead polluted soil eluent proposed by the present invention comprises the following steps:

[0029] S1. Take the inclined-plane PDA containing Aspergillus niger, rinse the surface of the inclined-plane PDA with sterile water to obtain the first mixed material, place the first mixed material in a sterile Erlenmeyer flask, and shake and cultivate it at a speed of 148rpm for 12.5h to obtain Aspergillus niger liquid, culture temperature is 28.5 ℃;

[0030] S2. Crush the tea seed cake to a particle size of 2-3 mm, add water and mix it, and then sterilize it at high temperature. The mass-volume ratio (g / L) of the pulverized tea seed cake to water is 4.5:9.2, and the high-temperature sterilization temperature is 120 ℃, the time of high temperature sterilization is 20min, after cooling to room temperature, add Aspergillus niger liquid, place it on a constant temperature shaker and cultivate it for 119h to obtain the second mixed mater...

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Abstract

The invention discloses a preparation method of a heavy metal lead-contaminated soil leachate. The preparation method comprises the following steps: taking inclined PDA containing aspergillus niger, rinsing the surface of the inclined PDA with sterile water to obtain a first mixture material, placing the first mixture material into a sterile conical flask, and performing shake cultivation for 11-13h to obtain an aspergillus niger solution; smashing tea seed cake to particle size of 2-3mm, adding water and mixing and then performing high-temperature sterilization, cooling to room temperature and then adding the aspergillus niger solution, culturing for 115-125h on a constant-temperature shaking table to obtain a second mixture material; thoroughly standing and precipitating the second mixture material, taking and adding supernatant liquid into lactic acid and evenly mixing to obtain the heavy metal lead-contaminated soil leachate. According to the preparation method, the raw materials are simply available, the preparation technology is simple, the preparation cost is greatly lowered, the leaching rate of lead in soil is high, and no secondary pollution to remedied soil exists.

Description

technical field [0001] The invention relates to the technical field of soil remediation, in particular to a preparation method of heavy metal lead polluted soil eluent. Background technique [0002] Due to the hidden, long-term and irreversible characteristics of soil heavy metal pollution, it is difficult to control once it is polluted. Over the past ten years in my country, phytoremediation technology for heavy metal-contaminated soil has attracted a lot of attention due to its low cost and environmental friendliness. However, heavy metal hyperaccumulating plants have the characteristics of slow growth, low biomass, special growth environment, limited by local climatic conditions and available plant species, and it is difficult to meet the requirements of rapid treatment of industrial wasteland. Therefore, the soil leaching technology with wide adaptability and rapidity has always been a difficult and hot research topic in the world, and has been widely studied in the lab...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C09K17/00B09C1/10B09C1/02
Inventor 熊华斌熊艳高云涛李晓芬王红斌熊勇罗利军杨志高金婷张继王根文吴怡颖
Owner YUNNAN MINZU UNIV
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