A kind of preparation method of heavy metal lead polluted soil leachate
A technology for lead-contaminated soil and eluent, applied in the field of soil remediation, can solve the problems of complicated preparation methods, high preparation costs, and low leaching rate
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Embodiment 1
[0018] A kind of preparation method of heavy metal lead polluted soil eluent proposed by the present invention comprises the following steps:
[0019] S1, take the inclined-plane PDA containing Aspergillus niger, rinse the surface of the inclined-plane PDA with sterile water to obtain the first mixed material, place the first mixed material in a sterile Erlenmeyer flask, and vibrate at a speed of 145rpm for 13h to obtain black Aspergillus liquid, culture temperature is 28 ℃;
[0020] S2. Pulverize the tea seed cake to a particle size of 2-3 mm, add water and mix it, then sterilize it at high temperature, add Aspergillus niger liquid after cooling to room temperature, place it on a constant temperature shaking table and cultivate it for 115 hours to obtain the second mixed material, the cultivation temperature of the constant temperature shaking table The temperature is 30°C, and the speed of the constant temperature shaker is 145rpm;
[0021] S3. After the second mixed materi...
Embodiment 2
[0023] A kind of preparation method of heavy metal lead polluted soil eluent proposed by the present invention comprises the following steps:
[0024] S1. Take the inclined-plane PDA containing Aspergillus niger, rinse the surface of the inclined-plane PDA with sterile water to obtain the first mixed material, place the first mixed material in a sterile Erlenmeyer flask, and vibrate at a speed of 155 rpm for 11 hours to obtain black Aspergillus liquid, culture temperature is 30 ℃;
[0025] S2. Pulverize the tea seed cake to a particle size of 2-3mm, add water and mix it, then sterilize it at high temperature, add Aspergillus niger liquid after cooling to room temperature, place it on a constant temperature shaking table and cultivate it for 125 hours to obtain the second mixed material, the cultivation temperature of the constant temperature shaking table The temperature is 28°C, and the rotation speed of the constant temperature shaker is 155rpm;
[0026] S3. After the secon...
Embodiment 3
[0028] A kind of preparation method of heavy metal lead polluted soil eluent proposed by the present invention comprises the following steps:
[0029] S1. Take the inclined-plane PDA containing Aspergillus niger, rinse the surface of the inclined-plane PDA with sterile water to obtain the first mixed material, place the first mixed material in a sterile Erlenmeyer flask, and shake and cultivate it at a speed of 148rpm for 12.5h to obtain Aspergillus niger liquid, culture temperature is 28.5 ℃;
[0030] S2. Crush the tea seed cake to a particle size of 2-3 mm, add water and mix it, and then sterilize it at high temperature. The mass-volume ratio (g / L) of the pulverized tea seed cake to water is 4.5:9.2, and the high-temperature sterilization temperature is 120 ℃, the time of high temperature sterilization is 20min, after cooling to room temperature, add Aspergillus niger liquid, place it on a constant temperature shaker and cultivate it for 119h to obtain the second mixed mater...
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