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A kind of breeding method of kumquat triploid

A technology for triploid and kumquat, applied in the breeding field of triploid of kumquat, can solve the problems of high technical requirements, difficulty in obtaining triploids, poor growth of regenerated plants, etc. Effect

Active Publication Date: 2016-07-27
GUANGXI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] The way to obtain triploids in the prior art usually adopts sexual hybridization and endosperm cultivation of tetraploids and diploids. Among them, it is difficult to obtain triploids for polyembryonic fruit trees due to the interference of nucellus embryos. , and the way to obtain triploids through endosperm cultivation has not yet been reported to successfully obtain triploid cultivars due to factors such as relatively high technical requirements and poor growth of regenerated plants.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment

[0033] The invention uses kumquat fresh food cultivated variety "Yangshuo kumquat" as mutagenic material. Specific steps are as follows:

[0034] (1) Tetraploid induction: select the plump seeds of Yangshuo kumquat ripe fruit, remove the outer seed coat, soak for 5 hours, peel off the inner seed coat, separate the seed embryo, and then soak it in a 0.2% colchicine solution 36h;

[0035] (2) Germination and cultivation of mutagenized seeds: take out the soaked seeds, and after cleaning, they will be germinated and rooted in a light incubator, moisturizing, and regularly observe the germination situation. After the roots are to be taken out, a 1cm root tip is cut and fixed in Carnot's fixative (95% ethanol: glacial acetic acid=3:1), serially numbered, and used for chromosome ploidy detection;

[0036] (3) Chromosome ploidy inspection;

[0037] (4) Cultivation of tetraploid plants: Select the number of embryos whose tetraploid check result in step (3) is tetraploid, and continue cultiv...

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Abstract

The invention discloses a breeding method for kumquat triploid. The method comprises the following steps: tetraploid mutagenesis, sprouting cultivation of seeds after mutagenesis, chromosome ploidy inspection, cultivation of tetraploid plants, tetraploid fruit harvesting and seed extraction, tetraploid embryo separation and cultivation, embryo chromosome detection, and cultivation of triploid seedlings. According to the method, a sexual hybridization process is avoided; the interference of nucellar embryo is eliminated; the triploid is directly separated and cultivated from polyembryonic tetraploid seeds; and the method has the advantages of being simple to operate, and short in breeding period.

Description

Technical field [0001] The invention belongs to the technical field of fruit tree genetic breeding, and specifically relates to a kumquat triploid breeding method. Background technique [0002] Kumquat belongs to the Rutaceae (Rutaceae) citrus subfamily (Aurantioideae), the genus Fortunella Swingle. There are 6 species and 1 variety of Kumquat. Kumquat is a general term for cultivated species. It can blossom and bear fruit many times a year. The fruit is beautiful in color, rich in nutrition, and has auspicious and festive meanings. It is a multi-purpose fruit tree for fresh food, processing and ornamentation. All species of the genus Kumquat are native to my country, but currently there are few varieties of kumquat cultivated, and there is only one type of fresh food. Yangshuo kumquat is currently a representative variety of fresh food with a large cultivation area and good economic benefits. [0003] Polyploid species is an important method for fruit tree breeding. Polyploid fr...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01H1/02A01H1/08
Inventor 黄桂香张树伟
Owner GUANGXI UNIV