Crocodile blood nano microcapsule and preparation method thereof

A technology of nano-microcapsules and crocodile blood, which is applied in the direction of micro-capsules, nano-capsules, food preparation, etc., can solve the problems of inconvenient storage, transportation, destruction of physiological activity, application fields and application market restrictions, etc., and achieve the extension of shelf life, preparation The method has the advantages of simple process and easy industrial production

Active Publication Date: 2015-04-08
XIAOGAN GUANGMEI TECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] The application of crocodile blood in existing patents and literature reports mostly destroys its physiological activity, and it is not convenient for storage and transportation, which greatly restricts its application field and application market
However, commercialized crocodile blood nano-microcapsule products have not been reported yet.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0037] 1) High-pressure homogeneous emulsification: under aseptic conditions, draw 1.8 kg of arterial blood from the neck of a healthy crocodile, put it into a blood collection bag containing 0.2 kg of 4% sodium citrate solution, and then shake it on a shaker After 45 minutes, take it off for later use; then pass the anticoagulant-treated crocodile blood material through a high-pressure homogenizer, and under the action of a high pressure of 420MPa, the crocodile blood solution passes through the homogenization chamber of the high-pressure homogenizer at a linear speed of 300m / s , the crocodile blood will be subjected to mechanical forces such as high-speed shear, high-frequency oscillation, cavitation and convective impact and corresponding thermal effects at the same time, and finally obtain a nano-homogenized emulsion of crocodile blood. The particle size of 90% of the particles in the crocodile blood nano homogeneous emulsion measured under the electron microscope is 80nm, ...

Embodiment 2

[0043] 1) High-pressure homogeneous emulsification: under aseptic conditions, draw 1.8 kg of arterial blood from the neck of a healthy crocodile, put it into a blood collection bag containing 0.2 kg of 4% sodium citrate solution, and then shake it on a shaker After 45 minutes, take it off for later use; then pass the anticoagulant-treated crocodile blood material through a high-pressure homogenizer. The crocodile blood will be subjected to mechanical forces such as high-speed shear, high-frequency oscillation, cavitation and convective impact and corresponding thermal effects at the same time, and finally obtain a nano-homogenized emulsion of crocodile blood. The particle size of 90% of the particles in the crocodile blood nano-homogenized emulsion measured under the electron microscope is 70nm, and the distribution is uniform.

[0044] 2) Initial coagulation: Add the crocodile blood nano-homogenized emulsion into a 10-liter glass three-necked bottle, and stir at a constant sp...

Embodiment 3

[0049] 1) High-pressure homogeneous emulsification: under aseptic conditions, draw 1.8 kg of arterial blood from the neck of a healthy crocodile, put it into a blood collection bag containing 0.2 kg of 4% sodium citrate solution, and then shake it on a shaker After 45 minutes, take it off for later use; then pass the anticoagulant-treated crocodile blood material through a high-pressure homogenizer. The crocodile blood will be subjected to mechanical forces such as high-speed shear, high-frequency oscillation, cavitation and convective impact and corresponding thermal effects at the same time, and finally obtain a nano-homogenized emulsion of crocodile blood. 90% of its particle size measured under an electron microscope is 60nm; and the distribution is uniform.

[0050] 2) Initial coagulation: Add the crocodile blood nano-homogenized emulsion into a 10-liter glass three-necked bottle, and stir at a constant speed of 70r / min, add 4kg of food-grade anhydrous ethanol as the init...

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Abstract

The invention discloses a crocodile blood nano microcapsule and a preparation method thereof. The crocodile blood nano microcapsule is prepared by the following method: treating fresh crocodile blood with an anticoagulant, then carrying out high-pressure homogenizing emulsification, and thus obtaining a crocodile blood nano homogenized emulsified liquid with the particle size of 30-100 nm; under stirring, adding ethanol, carrying out preliminary dehydration condensation curing; then under stirring, adding a carboxymethyl pachymaran aqueous solution, and carrying out simple coacervation; still under stirring, continuing to add a chitosan citric acid solution, carrying out complex coacervation, adding ethanol at a second time, and carrying out dehydration treatment; and finally, carrying out spray freeze drying, and thus obtaining the crocodile blood nano microcapsule. The crocodile blood nano microcapsule has no crocodile blood smell, not only maximize retention of a physiological activity, but also can perform targeted controlled release, and achieves targeted nutrition, health-care and food therapy effects; and the crocodile blood nano microcapsule not only is convenient to store and transport, but also can be widely applied in various fields. The preparation method has the advantages of simple process, and easy industrialization production.

Description

technical field [0001] The invention relates to the application of crocodile blood, in particular to a nano-microcapsule of crocodile blood and a preparation method thereof. Background technique [0002] Crocodiles are the only animals on earth that do not suffer from cancer, and they are the longest-lived reptiles. It has super vitality and the life characteristics of "immortality". Crocodile blood has the functions of sterilization, anti-radiation, and anti-aging. At the same time, it is also a brand-new, powerful super complement that does no harm to the human body. According to the latest research, the hemoglobin amino acid chain in its blood has a very miraculous structure, and its oxygen-carrying capacity is more than 100 times that of other animals. The blood contains extremely rich superoxide dismutase (SOD) and a super peptide ( SP), which has a strong ability to inhibit and kill tumor cells; in addition, scientists have extracted a super antibiotic—crocdilin from...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K35/58A61K9/51A61K47/36A61P35/00A61P37/04A61P31/04A61P17/16A23L1/30A23L1/09A61K8/98A61K8/73A61K31/715A23L29/30A23L33/10
CPCA23L2/38A23V2002/00A61K8/73A61K8/736A61K8/987A61K9/5161A61K35/58A61K47/61A61K2800/10A61Q19/00C12G3/04A23V2200/302A23V2200/30A23V2250/204
Inventor 蔡丽华鲁淑华戴俊峰
Owner XIAOGAN GUANGMEI TECH CO LTD
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