Composition for improving or promoting hair growth containing, as active ingredients, photosensitizer irradiated with light and peptide, and method using same
A sensitizer and peptide technology, applied in the field of hair growth improving or promoting compositions, can solve the problems of low hair growth effect, difficult, difficult to regenerate new hair follicles to play any role, etc., and achieves no cytotoxicity, low treatment effect of concentration
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[0105] Experimental materials and experimental methods
[0106] Manufacture of natural extracts through hot water extraction
[0107] Add 10 times the amount of primary distilled water to 100 g of dried Cimicifuga heracleifolia, Baikl skullcap and Lance Asiabell, extract at 100°C for 6 hours, and then filter. The filtered extract was freeze-dried, powdered, and stored at 4°C until use. Powdered natural extract, used in rat experiments at a concentration of 1mg / ml.
[0108] Production of ALA (5-aminolevulinic acid: 5-aminolevulinic acid)-peptide (GHK)
[0109]Into a glass reactor for peptide synthesis (DeaGyang Science, KOR) equipped with a filter element (0.1 μm; Sigma, USA), 14.286 g of 2-Chlorotritylresin (MERCK, USA) was mixed (replaced rate, 1.40mmole / g; 20mmole), Fmoc-Lys(Boc)-OH 18.742g (40mmole / g; MERCK, USA), DIEA (N,N-diisopropylethylamine) 13.97ml (80mmole / g; MERCK, USA) , add MC (methylene chloride; MERCK, USA) 400ml, and react for more than 5 hours. 10 ml of m...
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