Micro-fluidic chip device for separating bacteria by using laminar flow and chemotaxis effects

A microfluidic chip, chemotaxis technology, applied in biochemical cleaning devices, enzymology/microbiology devices, laboratory containers, etc., can solve the problems of low separation efficiency of separation chips

Inactive Publication Date: 2015-04-22
DALIAN UNIV OF TECH
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  • Application Information

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Problems solved by technology

Using the swimming and rolling characteristics of bacteria in laminar flow, people have established a variety of micr

Method used

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  • Micro-fluidic chip device for separating bacteria by using laminar flow and chemotaxis effects
  • Micro-fluidic chip device for separating bacteria by using laminar flow and chemotaxis effects
  • Micro-fluidic chip device for separating bacteria by using laminar flow and chemotaxis effects

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Embodiment Construction

[0036] The specific implementation manners of the present invention will be further described below in conjunction with the accompanying drawings and technical solutions.

[0037] figure 1 Shown is the structure diagram of the microfluidic chip device for isolating bacteria, including two parts: the induction reagent concentration control system I and the microfluidic chip for separating bacteria II. Among them, the induction reagent concentration control system I is composed of five sets of syringes and syringe pumps; the bacteria separation microfluidic chip II includes: induction reagent A inlet 9-1, bacteria inlet 9-3, induction reagent B inlet 9-2 , induction reagent A injection channel 9-4, bacteria injection channel 9-5, induction reagent B injection channel 9-6, bacteria separation channel 9-7, bacteria output channel 9-8, waste liquid output channel 9-9, bacteria collection Outlets 9-10 and waste outlets 9-11. Wherein, the entrance of the bacteria separation channel...

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Abstract

The invention belongs to the field of micro-fluidic chips, and discloses a micro-fluidic chip device for separating bacteria by using laminar flow and chemotaxis effects. The device comprises two parts namely a micro-fluidic chip for separating the bacteria and an inducing reagent concentration control system, wherein the micro-fluidic chip is an approximately 'X'-shaped PDMS-glass chip which is integrated with an inducing reagent inlet, a bacterium inlet, a bacterium separation channel, a bacterium collection outlet and a waste liquid outlet; and the inducing reagent concentration control system consists of a plurality of groups of injection pumps and injectors, parameters such as flow rates and the like can be controlled by virtue of the injection pumps, and thus an inducing reagent of which the concentration dynamically changes with time can be obtained. According to the micro-fluidic chip device disclosed by the invention, operations of separating interested bacteria from a bacterial mixed liquid can be achieved by mainly using the characteristics that the bacteria swim with tendency to high attractant concentration and low repellent concentration directions under the action of laminar flows and chemical inducing reagents. The micro-fluidic chip device disclosed by the invention can be applied to research and analysis of the fields of food safety, water quality monitoring, pharmaceutical engineering, clinical medicines and the like.

Description

technical field [0001] The invention belongs to the field of biological microfluidic chips, in particular to a microfluidic chip device for separating bacteria by using laminar flow and chemotaxis. Background technique [0002] Bacteria isolation refers to the process of isolating bacteria of interest from a mixture of bacteria and cells, or a mixture of various bacteria. It is of great significance in the food industry, water quality testing, bioengineering, pharmaceutical industry and clinical medicine. [0003] The traditional bacterial isolation is carried out in agar medium, the culture conditions are harsh, time-consuming, and the isolation and culture process is easily contaminated by miscellaneous bacteria, so the bacteria isolation and culture based on this method often cannot obtain ideal results. In recent years, the development of microfluidic technology has made the rapid isolation of bacteria possible. Using the swimming and rolling characteristics of bacteria...

Claims

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Application Information

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IPC IPC(8): C12M1/00B01L3/00
CPCC12M23/16C12M47/02
Inventor 覃开蓉于苗唐海英
Owner DALIAN UNIV OF TECH
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