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Immobilized enzyme carrier as well as preparation method and application thereof

An immobilized enzyme carrier and immobilized enzyme technology, which can be applied in the direction of immobilization on or in an inorganic carrier, can solve the problems of reducing the apparent enzyme activity, the limited types of functional groups on the surface of magnetic nanoparticles, and the deformation of the enzyme molecular structure.

Active Publication Date: 2015-04-22
LIVINGZONE SHANGHAI BIO CHEM TECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, the types of functional groups on the surface of magnetic nanoparticles are limited. Traditionally, covalent bonds or ion exchange forces are used to bind enzyme molecules, which often leads to deformation of the molecular structure of the enzyme and reduces the apparent enzyme activity.

Method used

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  • Immobilized enzyme carrier as well as preparation method and application thereof
  • Immobilized enzyme carrier as well as preparation method and application thereof
  • Immobilized enzyme carrier as well as preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0032] A preparation method for an immobilized enzyme carrier, comprising the following steps:

[0033] (1) Preparation of magnetic nanoparticles: Preparation of magnetic Fe by chemical co-precipitation method 3 o 4 Nanoparticles, the average particle size is controlled to be 50nm.

[0034] (2) Preparation of a hydrophilic sol-gel compound rich in carbon nanotubes: 1.0 g of agarose was dissolved in 45 mL of water, heated to boiling to form a sol. 0.5 g of carbon nanotubes (50 nm in length) were added to the boiling agarose solution, and ultrasonically dispersed at 220w at 80° C. for 30 minutes to obtain a hydrophilic sol-gel compound solution rich in carbon nanotubes.

[0035] (3) Carry out graft modification of carbon nanotubes to the magnetic nanoparticles prepared in step (1): add 0.5g of magnetic nanoparticles to 45mL of hydrophilic sol-gel compound solution rich in carbon nanotubes, 80 Ultrasonic dispersion at ℃ 220w for 30min to obtain magnetic Fe 3 o 4 , carbon nan...

Embodiment 2

[0049] A preparation method for an immobilized enzyme carrier, comprising the following steps:

[0050] (1) Preparation of magnetic nanoparticles: Preparation of magnetic Fe by chemical co-precipitation method 3 o 4 Nanoparticles, the average particle size is controlled to be 200nm.

[0051] (2) Preparation of a hydrophilic sol-gel compound rich in carbon nanotubes: 1.0 g of agarose was dissolved in 45 mL of water, heated to boiling to form a sol. 4.5 g of carbon nanotubes (250 nm in length) were added to the boiling agarose solution, and ultrasonically dispersed at 220w at 80° C. for 30 minutes to obtain a hydrophilic sol-gel compound solution rich in carbon nanotubes.

[0052] (3) Carry out graft modification of carbon nanotubes to the magnetic nanoparticles prepared in step (1): add 2.25g of magnetic nanoparticles to 45mL of the hydrophilic sol-gel compound solution rich in carbon nanotubes, 80 Ultrasonic dispersion at ℃ 220w for 30min to obtain magnetic Fe 3 o 4 , car...

Embodiment 3

[0066] A preparation method for an immobilized enzyme carrier, comprising the following steps:

[0067] (1) Preparation of magnetic nanoparticles: Preparation of magnetic Fe by chemical co-precipitation method 3 o 4 Nanoparticles, the average particle size is controlled to be 100nm.

[0068] (2) Preparation of a hydrophilic sol-gel compound rich in carbon nanotubes: 1.0 g of chitosan was dissolved in 45 mL of water, heated to boiling to form a sol. 0.45 g of carbon nanotubes (500 nm in length) were added to the boiling agarose solution, and ultrasonically dispersed at 220w at 80° C. for 30 min to obtain a hydrophilic sol-gel compound solution rich in carbon nanotubes.

[0069] (3) Carry out graft modification of carbon nanotubes to the magnetic nanoparticles prepared in step (1): add 0.45g of magnetic nanoparticles to 45mL of the hydrophilic sol-gel compound solution rich in carbon nanotubes, 80 Ultrasonic dispersion at ℃ 220w for 30min to obtain magnetic Fe 3 o 4 , carbon ...

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Abstract

The invention discloses an immobilized enzyme carrier as well as a preparation method and an application thereof. The immobilized enzyme carrier comprises a magnetic nanoparticle, a hydrophilic sol-gel compound and a carbon nano tube, wherein the surface of the magnetic nanoparticle is wrapped by the hydrophilic sol-gel compound to form a hydroxyl-rich shell; one end of the carbon nano tube is wrapped by the hydroxyl-rich shell; and the carbon nano tube is grafted on the surface of the magnetic nanoparticle. The immobilized enzyme carrier and the biological enzyme are combined, so that catalytic efficiency is high; recycling of enzyme is facilitated; and the industrial production cost can be effectively reduced.

Description

technical field [0001] The invention belongs to the technical field of preparation and application of solid-phase enzyme materials, and in particular relates to an immobilized enzyme carrier and its preparation method and application. Background technique [0002] Enzyme immobilization refers to an enzyme engineering technology that uses carriers to limit or bind enzymes in a certain area for catalytic reactions through physical and chemical methods. Immobilized enzymes maintain the unique catalytic activity of enzymes to a certain extent, and at the same time overcome the shortcomings of enzyme instability, easy inactivation, and difficulty in separation and purification, thereby reducing production costs. It is one of the most active research focuses in the field of enzyme engineering in recent years. , It has extremely broad application prospects in industrial production, chemical analysis and medicine. [0003] At present, the immobilization of enzymes mainly adopts ion...

Claims

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Application Information

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IPC IPC(8): C12N11/14
Inventor 祝俊余允东陈亭亭张敏汪浩张燕周晓青龙辉陈风义
Owner LIVINGZONE SHANGHAI BIO CHEM TECH CO LTD
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