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Method for detecting cyromazine

A detection method, the technology of cyromazine, is applied in the direction of material analysis by observing the influence on chemical indicators, and analysis by making materials undergo chemical reactions, which can solve the problems of long detection cycle, the need for professionals, and complicated operations. , to achieve the effect of low cost, short detection time and simple operation

Inactive Publication Date: 2015-04-22
INST OF QUALITY STANDARD & TESTING TECH FOR AGRO PROD OF CAAS
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, the current detection methods of cyromazine are all based on the instrumental analysis method of chromatography mass spectrometry, which has the disadvantages of long detection cycle, complicated operation, and the need for professionals, which is not conducive to the rapid spot check and on-site rapid detection of large quantities of samples, so it is urgent to develop a A simple and sensitive rapid detection method for cyromazine

Method used

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  • Method for detecting cyromazine
  • Method for detecting cyromazine

Examples

Experimental program
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Effect test

Embodiment 1

[0048] Embodiment 1, the optimization of NaCl concentration in the detection system

[0049] In order to obtain the best experimental results, the NaCl concentration in the detection system was optimized. Add 10 μL of NaCl solutions with different concentrations (0, 0.1, 0.2, 0.3, 0.4, 0.5, 0.6, 0.7, 0.8, 0.9, 1.0, 1.1, 1.2, 1.3, 1.4 and 1.5 M) into 150 μL of the system (the system Composed of 50 μL of the nano-gold solution and 100 μL of water), observe the color change of the nano-gold, and measure the absorbance value at 520 nm.

[0050] figure 2 A is the graph of the change of the color of nano-gold solution with the concentration of NaCl.

[0051] figure 2 B is the change diagram of the 520nm absorbance value of the nano-gold solution with the NaCl concentration.

[0052] from figure 2 It can be seen that as the concentration of NaCl added increases, the color of the nano-gold solution gradually turns blue. From the 520nm absorbance value, when the concentration o...

Embodiment 2

[0053] Example 2, Optimization of polyT10 concentration in the detection system

[0054] Different concentrations of polyT10 solutions (1, 1.5, 2, 2.5, 3, 3.5, 4, 4.5 and 5 μM) were incubated with 50 μL of the nano-gold solution for 5 minutes, then 50 μL of pure water and 10 μL of 0.9M NaCl were added, and then observed The color of the solution changes and the absorbance value at 520 nm is measured.

[0055] image 3 A is the graph of the change of the color of the nano-gold solution with the concentration of polyT10 at a specific NaCl content.

[0056] image 3 B is a diagram of the change of the 520nm absorbance value of the nano-gold solution with the concentration of polyT10 under a specific NaCl content.

[0057] like image 3 As shown, in the added polyT10 solution, the minimum concentration of polyT10 is 2.5 μM, which can protect the gold nano-aggregation induced by 0.9M NaCl and the color of the solution turns blue, so 2.5 μM polyT10 is selected as the polyT10 sol...

Embodiment 3

[0058] Embodiment 3, sensitivity and linear range of detection method

[0059] Firstly, 50 μL of 2.5 μM polyT10 was added to 50 μL of nano-gold solution, and after 5 minutes of reaction, polyT10 nano-gold solution was obtained; / mL) of cyromazine was added to it, and after another 5 minutes of reaction, multiple polyT10 nano-gold-cyromazine systems with different cyromazine concentrations were obtained. 10 μL of 0.9M NaCl was added and reacted for another 5 minutes to obtain multiple standard Solution, then observe the color change of the standard solution, and measure the absorbance value at 520nm.

[0060] Figure 4 A is a diagram of the change of the color of nano-gold solution with the concentration of cyromazine under a specific NaCl content and a specific polyT10 content.

[0061] Figure 4 B is a diagram of the change of the 520nm absorbance value of the nano-gold solution with the concentration of cyromazine under a specific NaCl content and a specific polyT10 conte...

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Abstract

The invention discloses a method for detecting cyromazine. The method comprises the following steps: preparing a polyT10@nanogold solution; adding cyromazine solutions with different concentrations, reacting, adding sodium chloride solution, reacting to obtain a standard solution, measuring the absorbance value of the standard solution at 520 nm, and drawing a standard curve for measuring the cyromazine content with the absorbance value as a vertical coordinate and with a cyromazine concentration logarithm as a horizontal coordinate; measuring the absorbance value of a solution to be measured at 520 nm, and obtaining the content of cyromazine in a sample to be measured according to the standard curve. The invention provides a quick, simple and sensitive method for measuring residual cyromazine in fruit and vegetable products, no complicated instrument, for example, chromatographic instrument-mass spectrometer, is needed, the operation is simple, and color change can be observed by naked eyes and can also be accurately quantified by measuring the absorbance. The detection time of the method is short, no toxic extraction reagent is needed, the costs of the used nanogold and aptamer are low, and the method can be used for quickly and early screening and inspecting a large number of samples on site.

Description

technical field [0001] The invention belongs to the technical field of rapid detection of pesticide residues, and in particular relates to a method for detecting cyromazine. Background technique [0002] Cyromazine is a triazine pesticide, which is widely used as an insecticide in the production process of animal breeding and fruit and vegetable planting, but its residues in animals and on fruits and vegetables can cause human tumors, and part of it can be metabolized It degrades into melamine and becomes a compound with nephrotoxicity. Therefore, in recent years, various countries have successively formulated the residue limit standard of cyromazine. For example, my country stipulates that the residue limit standard of cyromazine is 0.5-1μg / g. However, the current detection methods of cyromazine are all based on the instrumental analysis method of chromatography mass spectrometry, which has the disadvantages of long detection cycle, complicated operation, and the need for pr...

Claims

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Application Information

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IPC IPC(8): G01N21/78
Inventor 陈爱亮刘金钏白文荟朱超颜朦朦杨曙明
Owner INST OF QUALITY STANDARD & TESTING TECH FOR AGRO PROD OF CAAS
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