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Application of betamethasone 17-valerate in preparation of medicines for inhibiting tumor cell metastasis and spread

A technology of metasone valerate and tumor cells, which is applied to betamethasone valerate in the pharmaceutical field, can solve problems such as the pharmacological effects of betamethasone valerate that have not yet been discovered, achieve a large blocking effect, reduce the number of deaths, and develop research and development effect of cycle reduction

Inactive Publication Date: 2015-04-29
江苏澳格姆生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] Other pharmacological effects of betamethasone valerate have not been found in the existing published literature

Method used

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  • Application of betamethasone 17-valerate in preparation of medicines for inhibiting tumor cell metastasis and spread
  • Application of betamethasone 17-valerate in preparation of medicines for inhibiting tumor cell metastasis and spread
  • Application of betamethasone 17-valerate in preparation of medicines for inhibiting tumor cell metastasis and spread

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0024] Example 1: Application of betamethasone valerate in the preparation of drugs for inhibiting the metastasis and spread of colon cancer cells. The specific experimental plan is as follows:

[0025] 1. Experimental materials

[0026] Colon cancer cells (HT29) were used in the experimental stage of the present invention, and the drug used in the positive control was nocodazole (experimental concentration: 10uM). The negative control group received no treatment.

[0027] The experiment used betamethasone valerate compound C 27 h 37 FO 6 , CAS NO. is 2152-44-5, the concentrations are 100uM, 80uM, 60uM, 40uM, 10uM.

[0028] 2. Experimental method

[0029] For colon cancer cells cultured for 24 hours (cell density: 10 5 ) for drug stimulation for 2 hours:

[0030] (1) Centrifuge the colon cancer cells in a better state in the culture dish, and add an appropriate amount of culture medium to make a cell suspension;

[0031] (2) Get the cell suspension count, according to...

Embodiment 2

[0039] Example 2: Transwell cell migration assay was used to determine the effect of betamethasone valerate on the migration ability of colon cancer cells.

[0040] Will 1×10 5 The HT29 cells were inoculated in Transwell chambers with a 12.0 μm membrane at the bottom, and continued to be cultured. After the cells adhere to the wall, add DMEM complete medium with different concentrations of betamethasone valerate to the upper chamber. To maintain osmotic pressure, add 0.05%-0.2% BSA, and add DMEM complete medium containing 15% FBS to the lower chamber and continue Cultivate for 24h. The unmigrated cells on the upper surface of the membrane were carefully wiped off with a cotton swab, and the cells that migrated to the lower surface of the membrane were fixed with 2% PFA at 37°C for 10 minutes and then stained with Giemsa. Ten visual fields were randomly selected under a high-power microscope to count the migrated cells.

[0041] Cell migration rate = [the average number of c...

Embodiment 3

[0045] Example 3: The damage repair experiment detects the effect of betamethasone valerate on the migration ability of tumor cells.

[0046] Take HT29 cells in the logarithmic growth phase, and add 1×10 cells to each well of a 24-well plate 5 After the cells grow into a single layer, draw a "one"-shaped scratch, wash with PBS, add different concentrations of betamethasone valerate in the control group and the experimental group, and take pictures. After 22 hours of culture, the same part is taken again to measure the migration distance. The experiment was repeated 3 times, and the cell migration rate=[(0h average value of the experimental group-22h average value of the experimental group) / (0h average value of the blank control group-22h average value of the blank control group)]×100%.

[0047] Betamethasone valerate inhibited the migration of LTEP and A549 cells at different concentrations (10-100 μg / ml) for 22 hours, and the results showed that betamethasone valerate inhibit...

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Abstract

The invention discloses an application of betamethasone 17-valerate in preparation of medicines for inhibiting tumor cell metastasis and spread. The metastasis capability of tumor cells is inhibited by utilizing the medicines prepared from the betamethasone 17-valerate, the betamethasone 17-valerate refers to a low-toxicity medicine, a great blocking effect can be achieved in the tumor spreading process, meanwhile, treatment is performed by adopting other related tumor drugs, and the death amount of normal cells can be greatly reduced; and moreover, most of the existing tumor drugs are cytotoxic drugs, and lots of normal cells are killed when the tumor cells are killed.

Description

technical field [0001] The invention relates to a new application of betamethasone valerate in the field of pharmacy, in particular to the application of betamethasone valerate in the preparation of drugs for inhibiting tumor cell metastasis and spread. Background technique [0002] Betamethasone valerate is an adrenal corticosteroid drug. Has anti-inflammatory, anti-allergic and antipruritic effects. Neomycin sulfate has a local antibacterial effect and can inhibit most Gram-positive and Gram-negative bacteria, including Staphylococcus, Proteus and Pseudomonas, but has no effect on fungi. [0003] It is mainly used for external use of skin inflammation, inhibiting and alleviating skin itching, redness and swelling and other symptoms. Such as eczema, psoriasis and dermatitis. Betamethasone valerate is easily absorbed by the body, but if it is used for a long time, it will have side effects. [0004] Other pharmacological effects of betamethasone valerate have not been fo...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K31/573A61P35/04
CPCA61K31/573
Inventor 滕文臣
Owner 江苏澳格姆生物科技有限公司
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