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Preparation method of total saponin of dioscorea nipponica

A technology of total saponins and pangolin, applied in the field of extraction and separation of traditional Chinese medicine, can solve problems such as inability to enter the international market, low content of saponins or aglycones, and unstable quality

Inactive Publication Date: 2015-04-29
高文学
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

It mainly contains a variety of steroidal saponins such as dioscin. In recent years, studies have found that it has the functions of regulating the body's immunity, improving cardiovascular and cerebrovascular, anti-allergic, anti-rheumatism, relieving cough and expectorant, etc. Its aglycone is a synthetic adrenal cortex The main raw materials for hormones and oral liquid or injection contraceptives have a large market demand for their extracts, but the extracts are only crude extracts of Pangosaurus pangolinus, with low content of saponins or aglycones and unstable quality. Large energy consumption, no reasonable quality standards, unable to enter the international market

Method used

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  • Preparation method of total saponin of dioscorea nipponica

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0010] Example 1: The medicinal material of Pangosia chinensis is extracted 3 times with 10 times the amount of 65% ethanol, each time for 1.0 hour, and the ethanol is recovered and concentrated into a concentrated solution (concentrated to the point that each milliliter of the concentrated solution is equivalent to 1 g of the medicinal material). Put 100g of D101 macroporous resin in a separation column with a diameter-to-height ratio of 1:8, take 80ml of the concentrated solution after being adsorbed by the resin, and then elute with distilled water of 4 times the volume of the column bed at a flow rate of 1 times the volume of the column bed per hour , discard the eluate, and then use 5 times of column bed volume of 65% ethanol to elute at a flow rate of 2 times of column bed volume per hour, collect the eluate, recover ethanol, concentrate, dry under reduced pressure at 65°C, and pulverize. have to.

[0011] Sample quality evaluation:

[0012] (1) Determine the content of...

Embodiment 2

[0020] Example 2: Pangosia chinensis is extracted 3 times with 10 times the amount of 65% ethanol, each time for 1.0 hour, and the ethanol is recovered and concentrated into a concentrated solution (concentrated to the point that each milliliter of the concentrated solution is equivalent to 1 g of the medicinal material). Put 100g of D101 macroporous resin in a separation column with a diameter-to-height ratio of 1:6, take 100ml of the concentrated solution after being adsorbed by the resin, and then elute with distilled water of 4 times the volume of the column bed at a flow rate of 1 times the volume of the column bed per hour , discard the eluate, and then elute with 4 times of the column bed volume of 65% ethanol at a flow rate of 1 time of the column bed volume per hour, collect the eluate, recover the ethanol, concentrate, dry under reduced pressure at 65°C, and pulverize. have to.

[0021] Sample quality evaluation:

[0022] (1) Determine the content of diosgenin in th...

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Abstract

The invention discloses a preparation method of total saponin of dioscorea nipponica, and belongs to the field of extracting and separating effective components of natural medicines. The preparation method is characterized by comprising the following steps: adding 65% ethyl alcohol to dioscorea nipponica medicinal materials, performing extraction, recovering the ethyl alcohol from an extracting solution, performing concentration, adsorbing the concentrated solution through a D101 macroporous resin, separating the total saponin, and performing drying and crushing so as to obtain dry powder. The preparation method has the advantages that water and the ethyl alcohol which are used as a solvent in a preparation process can be recovered, so that pollution is not caused, the operation is simple and feasible, the preparation method is suitable for industrial large-scale production, the purity of extracts can reach more than 60%, the total saponin of the dioscorea nipponica can be used as food, medicines or raw materials in the respects of chemical industry and the like.

Description

technical field [0001] The invention relates to the field of extraction and separation of traditional Chinese medicines, in particular, the invention discloses a preparation method of total saponins of Pangosia chinensis. Background technique [0002] Chuanshanlong is a traditional Chinese medicine in my country. It is the rhizome of the perennial vine of Dioscorea genus Dioscorea. It is mainly used for rheumatic arthralgia, waist and leg pain, strain and sprain. It mainly contains a variety of steroidal saponins such as dioscin. In recent years, studies have found that it has the functions of regulating the body's immunity, improving cardiovascular and cerebrovascular, anti-allergic, anti-rheumatism, relieving cough and expectorant, etc. Its aglycone is a synthetic adrenal cortex The main raw materials for hormones and oral liquid or injection contraceptives have a large market demand for their extracts, but the extracts are only crude extracts of Pangosaurus pangolinus, wi...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K36/8945A61K125/00
Inventor 高文学张芳于晓刁兴斌王海燕
Owner 高文学